Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD.

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Presentation transcript:

Installing BioLinux on Mac OS X or Windows using a virtual machine Dr. Habil Zare, PhD

Bio-Linux Bio-Linux is a free workstation platform that facilitates running hundreds of bioinformatics tools without the corresponding installation hassles. These guidelines will help you installing Bio-Linux from the provided flash drive on your Mac or Windows computer. Instaling BioLinux using VM, Dr. Habil Zare 27 Oct

Requirements - A computer with at least 20 GB available space on the hard drive. A minimum of 4 GB of RAM is needed. - The installation can take minutes excluding the download time. Instaling BioLinux using VM, Dr. Habil Zare 27 Oct

USB flash drive To save time on installation, a USB drive is provided for the workshop. It contains the following files: 1.VirtualBox OSX.dmg VirtualBox Win.exe bio-linux-8-latest.ova Alternatively, you can download these files from VirtualBox and Bio-Linux, which may take several hours. VirtualBox Bio-Linux Instaling BioLinux using VM, Dr. Habil Zare 27 Oct

Step 0: Close all your programs. Instaling BioLinux using VM, Dr. Habil Zare 27 Oct BioLinux needs extensive memory and CPU resources. Close all other programs unless you have a fast, high-performance computer.

Step 1: Install VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS X

Step 1: Install VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Windows

Step 1: Install VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows

Step 1: Install VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows

Step 1: Install VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows

Step 1: Install VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows

Step 1: Install VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows

Step 1: Install VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows

Step 2: Run VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS X

Step 2: Run VirtualBox Instaling BioLinux using VM, Dr. Habil Zare 27 Oct The first time you run VirtuaBox, it looks like this:

Step 3: Import BioLinux Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows From the top menu, click on File>Import Appliance…

Step 3: Import BioLinux Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows

Step 3: Import BioLinux Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows Use the bio-linux-8-latest.ova file from the USB drive.

Step 3: Import BioLinux Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows Make sure, in the next step, you will check Reinitialize MAC address for all network cards. Both Mac OS X and Windows

Step 3: Import BioLinux Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows

Step 3: Import BioLinux Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows It can take minutes ….

Step 4: Turn on BioLinux Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Mac OS XWindows Double-click on Bio-Linux to turn it on.

Step 4.5: Enable VT-x Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Windows only In Windows, you may get an error like this the first time you run Bio-Linux. To fix it, you need to enable Virtualization Technology (VT-x) by modifying BIOS, which is a technical step. Restart while pressing F2, F10, F12, Esc, or DEL. Seek for help from a professional.

Step 4: Welcome to Linux world! Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Bio-Linux should appear after a black screen. From this point on, there is not much difference between Mac OS X and Windows.

Step 4: Welcome to Linux world! Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Maximize the corresponding window, or drag the bottom right corner of the screen to adjust it to your convenience. Mac OS XWindows

You are done! Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Bio-Linux is installed, and should work properly. -You do not need the USB driver any more. -When you need authentication information remember that Username: manger Password: manager

Faster analysis Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Note that the above approach uses a virtual machine (VirtualBox), which is appropriate only for educational purposes and running simple tests by beginners. For more complicated analysis, installing Bio-Linux on a flash or hard drive is recommended to increase computation speed.

What’s next? Instaling BioLinux using VM, Dr. Habil Zare 27 Oct Think of Bio-Linux as a new computer that you just got. Try to familiarize yourself with the new environment. Start by reading the Introduction to BioLinux, which can you do in 2 days.

References: These guidelines were based on a blog post by Dave Lunt. See the Bio-Linux website for documentation, training, and the list of supported software. I prepared these guidelines to facilitate the “Bioinformatics for biologists workshop” to be held on 20 Nov 2015, UTHSC – San Antonio. Instaling BioLinux using VM, Dr. Habil Zare 27 Oct