Today’s Plan: 9/16/09 Bellwork: Watch Video clip (10 mins) Finish Mutations and Operons (15 mins) Read/Pair/ Share about diabetes articles (15 mins) Read 1 insulin article. Your seat partner will do the other. Compare info and do journal –Why do diabetics need insulin? –What does insulin do? –What sources of insulin are available to diabetics? –What are the risks of using animal-derived insulin? Notes on Recombinant DNA and restriction enzymes (20 mins) Agriculture article/questions (the rest of the period) Pack/Wrap-up (last few mins of class)

Today’s Plan: 9/17/09 Bellwork: Ag applications article and questions (20 mins) Notes on RFLP and other technology (30 mins) RFLP Activity (20 mins) The rest of the period: –Gene Therapy Article and questions Pack/Wrap-up (last few mins of class)

Today’s Plan: 9/18/09 Bellwork: Q&A (10 mins) Vocab quiz and Unit 2 Test (as needed) If you finish early, work on missing tasks Pack/Wrap-up (last few mins)

Today’s Plan: 9/21/09 Bellwork: Blue Diamond #5 (20 mins) Portfolio Update (10 mins) Go over Friday’s test (35 mins) Finish test corrections and missing work (the rest of class) Pack/Wrap-up (last few mins of class)

Today’s Plan: 9/24/08 Bellwork: Re-cap on crime scene (10 mins) Crime Scene analysis (the rest of the period) Pack/Wrap-up (last few mins)

Today’

Recombinant DNA DNA can be manipulated to contain certain genes. Manipulations are performed by cutting DNA, inserting what we want, and inserting the DNA back into a cell. A manipulated piece of DNA, that contains “spliced” pieces is called recombinant DNA The recombinant DNA comes from cloning vectors-carrier DNA molecules that can be cut and spliced with new genes Most cloning vectors in biotechnology are bacterial plasmids

Transplanting Genes 1 st –cloning vector is removed from bacterial cells 2 nd -restriction enzymes that recognize specific base sequences on the DNA cut the plasmid at pre-determined sites, creating sticky ends 3 rd -the donor gene is inserted, closing the plasmid back into its ring shape 4 th -the new, recombinant DNA is inserted back into the bacterium. When the bacteria goes through binary fission, the donor gene is cloned too. The bacteria is now called a transgenic organism

Checking for Donor Gene Uptake As you know, not all laboratory exercises work, and scientists need a way to check to see if their transgenic bacteria picked up the donor gene. To test this, an antibiotic resistant gene is often also transferred with the desired donor gene. We can then grow the bacteria up on a plate containing the antibiotic in the agar. Only resistant bacteria survive, and these will also contain the donor gene that we’re looking for.

Other Uses of Restriction Enzymes Digesting DNA fragments for Fingerprinting. DNA is digested, loaded into a gel, and you end up with bands of DNA Bands are characteristic of each person, and can be used as a “Fingerprint” (also known as RFLP= Restriction Fragment Length Polymorphism)

Uses of DNA Technology Gene Therapy-fixing genes with mistakes in an adult organism PCR-Copying mass quantities of DNA fragments Cloning-using nuclear switches of stem cells to create identical organisms Human Genome Project (HGP)- Sequencing and Mapping all of the genes in the human genome.

Test Topics Steps to creating transgenic organisms Purpose of each step Steps of cloning Uses of stem cells Pros/Cons of human gene therapy Steps to RFLP analysis Uses for RFLP