INTRODUCTION & BACKGROUND

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Presentation transcript:

Multidimensional Spectroscopy Laboratory (MSL) Korea Basic Science Institute December, 2008

INTRODUCTION & BACKGROUND 2

Protein Structure Determination: Tools and Comparison Advantages and Limitations  Researchers use a variety of tools to probe protein function and interactions, with drug discovery the major goal  One of seven research fields “Large-scale protein folding and 3-D structure studies” Advantages Restrictions Molecular crystal & Low time- resolution X-ray crystallography High spatial resolution Low time- resolution 2D-NMR Solution M. Cho, Chem. Rev. 108, 1331 (2008)

exposures and fast enough framing rates for an event to be evaluated High speed photography “Recording optical or electro-optical information with adequately short exposures and fast enough framing rates for an event to be evaluated with a temporal and dimensional resolution which satisfies the experimenter” Eadweard Muybridge (1887)

Molecular Motion Pictures M. Cho, Nature 444, 431 (2006)

VIS optical diffraction limit Structure space (m) Dynamics time (sec) 10-9 10-6 10-3 10-12 10-15 XRD EM OM NMR MDS TRS VIS optical diffraction limit

High Competition & state-of-the-art

Experimental Labs World-wide International: R. Hochstrasser (U. Penn) G. R. Fleming (U. California Berkeley) M. D. Fayer (Stanford) A. Tokmakoff (MIT) M. Zanni (U. Wisconsin Madison) P. Hamm ( U. Zurich) + 10 research labs Asia: None Domestic: KBSI

Experimental Setups (KBSI)

2D photon echo spectrometer (the only facility in Asia)

Nonlinear optical spectroscopy: Experimental setup spectrometer coh. time pop. time echo time 1 2 3 4 t T t spherical mirror 4=LO 1 2 3 sig sample OD3 4 1&2 3 3&4 2 1 diffractive optic (DO) delay 2 delay 1

2D photon echo experimental scheme

Multidimensional Spectroscopy Laboratory (MDSL)

Multidimensional Spectroscopy Laboratory (MDSL) H-bond making H-bond breaking kb 14

Femtosecond CD and ORD spectrometer (novel technique)

H. Rhee, Y. -G. June, J. S. Lee, K. -K. Lee, J. -H. Ha, Z. -H. Kim, S H. Rhee, Y.-G. June, J.S. Lee, K.-K. Lee, J.-H. Ha, Z.-H. Kim, S.-J. Jeon, M. Cho NATURE, in press (2008)

Detailed experimental schemes and Results Femtosecond spectra interferograms Vibrational circular dicrhoism Vibrational optical rotatory dispersion 17

Time-resolved CD and ORD in femtosecond timescale! 18

Femtosecond Difference Frequency Spectroscopy Novel Vibrational Optical Activity Measurement Technique

Major achievements 1. 2D IR photon echo setup (아시아 유일의 실험장비) Chemical Reviews (108, 1331, 2008): IF = 26 2. Femtosecond vibrational CD and ORD (세계 최초의 장비 및 실험, 국내-국제 특허 출원) Nature, in press (2008) J. Chem. Phys. 129, 094507 (2008) 3. Novel nonlinear spectroscopy Time-resolved DFG (Chirality!) Nonlinear optical imaging

Vision and Plans

Long term projects

Pulse shaping에 의한 다차원 실험 기법 개발 ’08 ’09 ’10 ’11 ’12 다차원 분광기 개발 분광분석 시스템 Products 생체분자 동역학 분석기술 수월적 연구 성과 최첨단 레이저 분석 기술 확보 첨단 분석기기 개발 기술확보 다차원분광기 개발 온도 점프 장치 개발 적외선 다차원 실험기법 개발 순간 여기 다차원 실험기법 개발 Pulse shaping에 의한 다차원 실험 기법 개발 두 색 다차원 및 새로운 시퀀스 분광기 개발 단순 펩타이드계에 대한 동역학 분석기술 개발 복잡 단백질에 대한 동역학 분석기술 개발 (세부목표명) 성과지표명

(Multi-dimensional Nonlinear Spectro-Microscopy and BioDynamics) 전략적 미래융합과학 연구 프로젝트 중 신규 전략적 융합 과학과제 타 출연(연)과 차별화 및 타 지역 센터와 차별화 연구 지원사업 전문사업단으로 발전을 지향하는 미래 지향적인 융합 과학 과제 육성 다차원 나노 비 선형분광 및 생체 동역학 (Multi-dimensional Nonlinear Spectro-Microscopy and BioDynamics) 비 선형광학, 분광학 (기존의 물리, 화학) + Microscope (NT) + 생체물질, 현상 (BT) 세계 최고 수준의 생체 동역학 연구를 위한 Nonlinear Spectrophotometer 개발 24

Ultimate goals

생명 현상의 이해 분자수준에서의 구조-활성 연관성 연구 독립된 생체분자의 구조/동역학 연구 수십나노미터 수준의 생체 군집의 구조/활성 비선형 다차원 분광학의 이론 및 실험적 연구 화학선택적 이미징이 가능한 분광학적 현미경 실험 Multidimensional spectroscopy with time-space resolution Biostructure and Biodynamics 26

Chemical exchange and reaction dynamics protein aggregation process In summary 다차원 분광법에 대한 실험법과 데이터 해석법의 확립을 통하여 단백질, 핵산과 같은 생화학물질의 구조와 동역학 연구법 개발 Chemical exchange and reaction dynamics protein aggregation process Protein folding DNA and RNA dynamics 27

Molecular Motion Pictures AGAIN! Novel Spectroscopic Techniques (KBSI-Seoul Center) 2D IR spectroscopy fs CD and ORD Experimentally demonstrated 3. fs optical activyt-DFG New proposal M. Cho, “Spectroscopy, Molecular Motion Pictures”, Nature 444, 431 (2006) M. Cho, “Coherent two-dimensional optical spectroscopy, Chem. Rev. 108, 1331 (2008)