Immune system Chapter 43

Pathogen: Infectious agent Innate immunity: Nonspecific Acquired immunity: Specific Previous exposure

Defense First line Second line Third line Skin Cell counterattack Immune response (antibodies)

First-line Skin 1. Impenetrable barrier 2. Oil & sweat glands Skin pH at 3-5 3. Sweat contains lysozyme Enzyme that digests bacterial walls 4. Prevents water loss

Skin

First line Lysozyme in saliva Acid in stomach Digestive enzymes in gut Mucous in airways Cilia in airways Acidic urine

First line

Trachea

Second line Invaders Lymphatic system Defense cells Adenoids, tonsils Thymus, spleen Lymph nodes, lymph capillaries & ducts

Lymphatic system

Second line WBC Antimicrobial proteins Inflammatory response

Second line Leukocytes (WBC) Circulating in the body 1. Macrophages (monocytes) Kill invaders by ingesting them Phagocytosis Ingestion

Microbes PHAGOCYTIC CELL Vacuole Lysosome containing enzymes Fig. 43-3 Figure 43.3 Phagocytosis

Second line 2. Neutrophils Most abundant WBC Phagocytosis Release chemicals that kill bacteria Also kills other neutrophils

Second line 3. Eosinophils WBC Low phagocytic activity Parasite defense

Second line Antimicrobial proteins 1. Complement system Proteins found in plasma Attack bacterial or fungal cell walls Cause cells lysis Signals other defense responses

Second line 2.Interferons Paracrine polypeptide Protect cells in area of virus Prevent viral replication Cancer defense

Second line 3. Natural killer cells Kill cells that are infected by a virus Help fight cancer cells

Second line Inflammatory response Local, non-specific Histamines & prostaglandins Vasodilation Increased blood flow to area Edema or swelling WBC (phagocytic), pus formation Shock, systemic

Second line

Second line Inflammatory response Elevated temperature (fever) Interleukin-1 Released by Macrophages Directs hypothalamus to increase temp Helps stimulate defense response

Third line Specific response Lymphocytes (B-cell orT-cell) Antibodies

Third line Antigen Molecule that causes a specific immune response Foreign to body Located on surface Epitope Antigen determinant

Third line Self-versus-nonself recognition Genes code for specific proteins Major Histocompatibility Complex proteins MHC proteins Cell recognition Glycoproteins on surface of cells

Third line B-cell lymphocytes Made & develops in bone marrow Becomes a plasma cell Produce antibodies in response to specific antigens Immunoglobulins (Ig) Antibodies Humoral immunity

E:\Chapter_43\A_PowerPoint_Lectures\43_Lecture_Presentation\43_14RoleOfBCells_A.html E:\Chapter_43\A_PowerPoint_Lectures\43_Lecture_Presentation\43_21Antibodies_A.html

Third line B-lymphocyte structure Antigen receptor Specific Plasma membrane

Third line Light chains 2 short polypeptides Heavy chains 2 identical long polypeptides 4 chains held together by disulfide bond Forms Y-shaped molecules

Antigen- binding site Antigen- binding site V Disulfide bridge V V V Fig. 43-9a Antigen- binding site Antigen- binding site V Disulfide bridge V V V Variable regions C C Constant regions C C Light chain Transmembrane region Figure 43.9 Antigen receptors on lymphocytes Plasma membrane Heavy chains B cell Cytoplasm of B cell (a) B cell receptor

Antigen-binding sites Fig. 43-10 Antigen- binding sites Epitopes (antigenic determinants) Antigen-binding sites Antibody A Antigen V V Antibody C V V C C C C Figure 43.10 Epitopes (antigenic determinants) Antibody B

Antibodies Plasma cells release antigen receptor Specific for antigens Arms of the Y shaped molecule Have different aa sequences

Antibodies IgM first response Aggregation of complement proteins IgG major form, second response Stimulates phagocytosis by macrophages IgD receptors for antigens on B cells IgA Present in breast milk, mucous, saliva Provide protection to newborns

Antibodies IgE Release histamines Bind to mast cells Insert heavy chain into mast cells Initiate inflammatory response Presence of antigens Vasodilation

Antibodies

E:\Chapter_43\A_PowerPoint_Lectures\43_Lecture_Presentation\43_18CytotoxicTCells_A.html E:\Chapter_43\A_PowerPoint_Lectures\43_Lecture_Presentation\43_17HelperTCells_A.html

Third line T-cell lymphocytes Made in bone marrow Processed in thymus gland Regulate immune responses Attack cells with specific antigens Cell-mediated immunity

Antigen- binding site Variable regions V V Constant regions C C Fig. 43-9b Antigen- binding site Variable regions V V Constant regions C C Transmembrane region Figure 43.9 Antigen receptors on lymphocytes Plasma membrane  chain  chain Disulfide bridge Cytoplasm of T cell T cell (b) T cell receptor

Third line T-cells 1. Helper T cells (CD4) Initiate response based on antigens 2. Memory T cells Remember previous antigens

Third line 3. Cytotoxic T cell (CD8) Lyse cells infected by virus 4. Suppressor T cells Turn off immune response

Third line response Antigen Macrophage process antigen Secrete cytokines (interleukins or interferons) Stimulates T helper cells

Third line response Recognize antigens Antigen receptor on T-cells Bind to antigens Triggers T-cytotoxic cells, T-memory cells Cytotoxic cells destroy infected cells Stimulates B cells

Third line response Antigen receptor on B-cell Binds foreign antigen Triggers formation of a clone of plasma cells Clones produce antibodies Antibodies bind invading antigen Prevent affects of antigen Destruction or blocks effect

Immune response Macrophage ⇓ Helper T-cell ⇙ ⇘ B-cell Cytotoxic T-cell ⇙ ⇘ B-cell Cytotoxic T-cell ⇙ ⇘ ⇙ ⇘ Plasma Memory Memory Cytotoxic cells cells cells T-cells Antibodies

T-cells Cytotoxic T-cells Attack transplants (skin grafts) Considered foreign tissue Destroy cancer cells Interferon (lymphomas, renal Ca, melanoma, Kaposi’s sarcoma and Breast Ca) Interleukin (tx cancer)

B-cells Primary immune response First exposure Lasts about 2 weeks Memory cells are also produced during the first exposure Secondary immune response Activates memory cells Response faster & lasts longer

Immune System Summary First line Skin, cilia, enzymes, pH of skin Second line WBC (macrophages, neutrophils, natural killer cells, eosinophils) Antimicrobial proteins Inflammatory response

Immune System Summary Third line Lymphocytes (B & T) Antibodies (immunoglobulins) IgM, IgG, IgA, IgE, IgD

Monoclonal antibodies Antibodies specific for one antigen Cell cultures produce large quantities Use in lab tests Pregnancy tests Antibody to HCG (human chorionic gonadotropin)

Immunity Acquired immunity Active Disease Vaccine Transfer of lymphocytes from a donor (bone marrow transplant) Passive Maternal antibodies Immunoglobulins (gamma globulins)

HIV Human immunodeficiency virus Attacks + destroys CD4+ T-cells T-cells secrete a suppressing factor Blocks other T-cells Infects macrophages & brain cells

HIV

E:\Chapter_43\A_PowerPoint_Lectures\43_Lecture_Presentation\43_26HIVReproCycle_A.html

AIDS Acquired immunodeficiency syndrome Opportunistic infections Pneumocystis carinii Kaposi’s sarcoma Neurological damage Physiological wasting

Autoimmune diseases Systemic Lupus Rheumatoid arthritis Hashimoto thyroiditis

Allergy Allergens (antigens) Release IgE Binds mast cells & basophils Stimulates release of chemicals Histamine Drop in BP

Allergy Anaphylactic shock Widespread histamine response Death Bee stings or peanuts or penicillin Contact dermatitis Delayed response Poison ivy, poison oak

Blood types (ABO) Blood type Surface antigens Antibodies A anti B B anti A AB none O anti A & B

ABO

Rh factor