Analysis of Env binding of Fluor-conjugated antibodies (A. Kliche)

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Analysis of Env binding of Fluor-conjugated antibodies (A. Kliche)

ConS gp140 #86 binding to A32 sTime RU Response A Binding to ConS gp140 #86 sTime RU Response B12 - A32 B12-ALEXA488- A32 Req ConS 140 #86 - A32332 ConS 140 #86 - A32331 Req Ab binding ratio ConS 140 #86 + B12 - A ConS 140 #86 +B12-ALEXA488- A ConS 140 A32 Ab A32 Control IgG

ConS gp140 #86 sTime RU Response A Binding to ConS gp140 #86 sTime RU Response HGN194 HGN194-ALEXA488 Req ConS 140 #86 - A32327 ConS 140 #86 - A32311 Req Ab binding ratio ConS 140 #86 + HGN194 - A ConS140+HGN194-ALEXA488 - A ConS 140 A32 Ab A32

ConS 140 CFI Capture HJ16 HJ16 Alexa Fluor ConS 140 CFI Capture HJ16 HJ16 Alexa Fluor ConS 140 CFI Capture HJ16 HJ16 Alexa Fluor 488 Binding Response (RU) Time (s) ReplicateCapture Sample Binding Response (RU) Binding Antibody Binding Response (RU) Binding Ratio 1A32_ConS 140 CFI303HJ A32_ConS 140 CFI318HJ A32_ConS 140 CFI253HJ A32_ConS 140 CFI304HJ16 Alexa Fluor A32_ConS 140 CFI316HJ16 Alexa Fluor A32_ConS 140 CFI215HJ16 Alexa Fluor