13-15 July 2015– Beijing Kinetics of biomass and hydrocarbon oils production of microalgae Botryococcus braunii in continuous culture.

Slides:



Advertisements
Similar presentations
By: Schmeltz Vanessa ; Di Pascoli Thomas
Advertisements

AlgaePARC biorefinery
BioEnergy Production from Food Waste
Powering the Future: Biofuels. Activity: Algae Chromatography Extract pigment from algae Separate and compare the pigments in red and green algae Analyse.
Powering the Future: Biofuels. Activity: Algal Photosynthesis Describe the requirements of photosynthesis Take measurements to assess the rate of photosynthesis.
Growing Chlorella for Algae-Oil Biofuels and Aquaculture Feeds Aquaculture Sustainability Conference Yantai, China Kevin Fitzsimmons and George Lin.
Second generation Biofuels Beyond Oxygenates Workshop session on Biofuel sources and supply May 2, 2008, Brookhaven National Laboratory.
Biomass as a Fossil Fuel. Biofuel (also called agrofuel) can be defined as solid, liquid, or gas fuel consisting of, or derived from biomass. Biofuels.
What is a hydrocarbon? Why are alkanes considered to be saturated?
THINK OUTSIDE THE BARREL …
International Network on Biofixation of CO2 and International Network on Biofixation of CO2 and Greenhouse Gas Abatement with Microalgae Greenhouse Gas.
Fossil Fuels, Ethanol, and Biodiesel By: Emma Wellman, Vishal Garg, and Tom Barch Seeking a responsible solution to global warming by decreasing greenhouse.
Calderglen High School.
Algae to Biodiesel Joshua J. Heller Department of Chemical Engineering The University of Texas at Austin.
Rebecca Crabtree Fall 2010  Green House Gases (GHG) Carbon Dioxide (CO 2) Nitrous Oxide (N 2 O) Methane (CH 4 )  Lowered pH of oceans Acidity= loss.
Title: Coal Cowboy Duration: 00:12:51 Link: engr
Ahmed Atta A Introduction  Algae are a diverse group of primarily aquatic, single celled, plant like organisms. Most algae have characteristics.
Broadly biodieselis composed ofCarbon - 77%,Hydrogen -12%,Oxygen - 11% andtraces of Nitrogenand Sulfur(Tomasevic et al,2003) Advantages –Renewable,biodegradable,less.
Maximum sustainable photosynthetic efficiency, biomass productivity and oil productivity will be determined Capital costs of microalgae cultivation systems.
B i o – D i e s e l Oil From Algae “The First Industrial Plant”
Speaker: Jeng-Chen Liu(劉政成) Student ID: P
Israel's Role in Reducing Global Oil Dependency Alternative Liquid Fuels M. Herskowitz Blechner Center of Industrial Catalysis and Process Development.
PERFORMANCE AND EMISSION CHARACTERISTICS OF A NON EDIBLE OIL(BIODIESEL) IN A DI ENGINE USING DEE IN DUAL FUEL MODE PROJECT MEMBERS : S.SARAVANAN
Acknowledgements We are grateful to our mentor, Dr. Christine Case, for her advice throughout this project. Her great advice and her enthusiasm about biology.
RL Stevenson Presentation Biological Fuels Daniel M. Jenkins University of Hawai‘i, Mānoa April 27, 2007.
Production of Biodiesel from microalgae Biodiesel Production from LUIS BELO MORAIS- PERFECT WAVE Microalgae.
Cost reduction for biodiesel production from distillery/domestic mixed wastewater by Rhodosporidium toruloides Jiayin Ling, Renata Alves de Toledo, Yuan.
Feedstocks Cull Potato  Underutilized agricultural biomass with low commercial value  Can provide both carbon and nitrogen sources for algae’s growth.
Bioengineering the Future
Growing Algae for Biofuel Søren Laurentius Nielsen Department of Environmental, Social and Spatial Change.
Abstract Our project is based on the idea of exploring algae bio- fuel technology. During our research we discovered that scientists haven’t compared algae.
High density culture of Aurantiochytrium limacinum SR21 for DHA production with crude glycerol from biodiesel industry Zhanyou Chi, Craig Frear, Shulin.
設計嗜熱藍綠菌專用薄板反 應器以提高生質體產率及二 氧化碳固定速率 朱逸鈴 環境工程學系碩士班 李姿穎 環境工程學系學士班 闕凡瑜 環境工程學系碩士班 林鈺婷 環境工程學系碩士班 江允中 環境工程學系碩士班 編號 : 101A12.
Abstract: The use of renewable energy sources is becoming increasingly necessary to mitigate global warming. Recently much research has been focused on.
Title: Beyond Biofuels NOW! Feasible, affordable, scalable, sustainable… Not use agricultural land Not use freshwater.
Zhanyou Chi Department of Biological Systems Engineering,
Micro Algae Production: A Renewable, Sustainable Alternative to Produce Fuels and Fertilizers Ganti S. Murthy Biological and Ecological Engineering Department.
Table 1: Comparison of R. javanicus cultivation data and PDC production for 500 ml Erlenmeyer flask, 5 and 30 l bioreactors. The variables measured include:
Nutrient recovery from anaerobic co-digestion of Chlorella vulgaris and waste activated sludge Michael Gordon 1, Tyler Radniecki PhD 2, Curtis Lajoie PhD.
Development of integrated bioprocess for ethanol production from sugar beet Dr. sc. Božidar Šantek, Full Professor Department of Biochemical Engineering,
Biofuels.
SETAC Conference November 2013 Rome, Italy
REG WALTERS Board Member & Managing Director SQC Pty Ltd Board Member & Managing Director SQC Pty Ltd SQC.
(Picture from Martek Annual Report) Oil Crops Algal Fermentation Process Cow Stomach “Extractor” Cull Potatoes Omega-3 food Biodiesel Crude Glycerol Feed.
BioDiesel from Algae An Integrated Approach. any of various chiefly aquatic, eukaryotic, photosynthetic organisms, ranging in size from single-celled.
Environment Energy Agri-food Sea Centre National de la Recherche Scientifique LABORATORY OF PROCESS ENGINEERING FOR ENVIRONMENT AND FOOD.
Bioenergy Basics 101 Biobenefits Check Your Source Fueling the Future From Field To Pump The Raw Materials Fun in the Sun
E FFECT OF F LUE G AS ON A LGAE G ROWTH By Esteban Jimenez Mentored by Dr. Kimberly Ogden Department of Chemical and Environmental Engineering Saturday,
Biomass/Biofuel/Biogas
RESEARCH LABORATORY OF BIOENERGY (RLB) Department of Chemistry, Federal Urdu University of Arts, Science and Technology, Gulshan-e-Iqbal Campus, University.
Algae Lipids Protein Carbohydrate Anabaena cylindrica 4–7 43–56 25–30 Aphanizomenon flos-aquae Arthrospira maxima 6–7 60–71 13–16 Botryococcus.
Development of an integrated algal bio-refinery for polysaccharide and bio-fuel production Cesar Moreira 1, Murali Raghavendran 2, Yatin Behl 2, Spyros.
The use of algae as an alternative source of bioenergy (e.g., for making biogas or bioethanol) has a large potential. Viewing algae as a photosynthetic.
A better understanding of culture parameters to increase exopolysaccharide production by Botryococcus braunii CCALA 778 Rafael G. Cubero, Dorinde Kleinegris,
Powering the Future: Biofuels. Activity: Culturing algae Describe the requirements for algal growth Culture algae in flasks or on agar Discuss the difficulties.
Algae Biomass Production for Fish Feed and Fuel Qi Li 1 Dr. Pete Waller 1 Joni Lee Giovanna Hesley 1 Randy Ryan 2 Dr. Kevin Fitzsimmons 2 Brunno Cerozi.
Workshop on: Green Technologies & Energy Efficiency April 26, 2017
CERTH DIESEL SUSTAINABILITY IMPROVEMENT PERSPECTIVES BY INCORPORATION OF WASTE COOKING OIL IN EXISTING REFINERY OF THESSALONIKI Stella Bezergianni, Athanasios.
CHAPTER 3: HyDROGEN GENERATION BY MICROBIAL CULTURES
Chapter 4: Biofuels from Algae and Seaweeds
In Conjunction With: National Science Foundation Community College Innovation Challenge 2015 “Optimizing Genetic Engineering Technology for Increased Lipid.
 No fertilisers needed
IIT Bombay 5th International Conference on Advances in Energy Research
Fatty acid productivity of Scenedesmus obliquus under nitrogen starvation in mixotrophic cultivation exceeds the combination of autotrophic and heterotrophic.
Supervisor :Dr. Robert Edyvean and Dr. Stephen Wikinson
What is a hydrocarbon? Why are alkanes considered to be saturated?
tSmax = instant when the starch productivity reach the maximum.
EFFECTIVENESS OF COMO AND NIMO CATALYST ON CO-HYDROPROCESSING OF HEAVY ATMOSPHERIC GAS-OIL/WASTE COOKING OIL S. Bezergianni A. Dimitriadis Laboratory of.
Algae as a Biofuel Advisors: Dr. Kauser Jahan Dr. William Riddell
Tek. Bioenergi (TKK-2129) Instructor: Rama Oktavian
Presentation transcript:

July 2015– Beijing Kinetics of biomass and hydrocarbon oils production of microalgae Botryococcus braunii in continuous culture Jian JIN, Catherine DUPRE, Jack LEGRAND, Dominique GRIZEAU * GEPEA, Université de Nantes, CNRS, UMR 6144, CRTT, 37 bd de l’Université, BP 406, Saint-Nazaire Cedex, France 7 th Asia Pacific Biotech Congress

Summary 2 7th Asia Pacific Biotech Congress 1. Background and review 2. Continuous culture setup 3. Results and discussion 4. Conclusions and perspectives

1. Background and review 7th Asia Pacific Biotech Congress 3

4  Sustainable production of bio-fuel Alternative bioresource to produce bio-hydrocarbon ? 1.Background and review : Petroleum crisis, biofuel and bio-hydrocarbon 3rd generation biofuel = microalgae efficient & no competition with food  Triacylglycerol for biodiesel  But also bio-hydrocarbons Advantages of bio-hydrocarbon = main components of fossil fuels  High caloric value  Without oxygen  Jet fuel & Compatible with existing combustion engine

5 7th Asia Pacific Biotech Congress OrganismContent (%)Reference Animal Gołębiowski et al., 2011 Plant Huang et al., 2011 Bacteria Jones, 1969 Fungi Ladygina et al., Background and review : Hydrocarbons present in many organisms An outstanding microalga Botryococcus braunii Organism Content (%) TypeReference Aurantiochytrium sp.19Heterotroph Kaya et al., 2011 Vibrio furnissii60Heterotroph Derber et al., 2011 Botryococcus braunii61Autotroph Metzger et al., 2005 Most organisms = hypo-producers A few organisms = hyper-producers Bar: 10µm (Suzuki et al., 2013)

6 7th Asia Pacific Biotech Congress ( Weiss et al., 2012; Metzger et al., 2005; Kawachi et al., 2012) Biological model = B. braunii Race A 1.Background and review : Colonies, extracellular matrix and diversity Colony : Cells aggregation  easy for harvest Extracellular matrix : HC accumulation  milking for extraction HC diversity: >60 strains and 4 races  strain selection ( Bot 22 ) Race B Race L Race S Potential but under-studied cell factory e.g. hydrocarbon productivity and N-limitation ? e.g. hydrocarbon productivity and environmental pH? Hydrocarbon Bar: 2 µm

pH 7 7th Asia Pacific Biotech Congress  pH and enzymatic activities  pH and inorganic carbon speciation  pH and macromolecules transportation between cell and its extracellular environment ( Guo et al., 2006) 1.Background and review : Studies of pH effects on B. braunii Previous studies of pH effects on B. braunii In Erlenmeyer but not in controlled photobioreactor (PBR) Initial pH adjustment without continuous pH control  pH increased during cultivation (Dayananda et al., 2006 & 2007) pH HCO 3 - H 2 CO 3 CO 3 2-

8 7th Asia Pacific Biotech Congress 1. Background and review : Other studies in pH controlled photobioreactors Only two studies with pH control in PBR in batch cultures StrainRace pH control Biomass productivity (g L -1 d -1 ) Hydrocarbon productivity (g L -1 d -1 ) Total lipids productivity (g L -1 d -1 ) Reference GUBIOTJTBB Talukdar et al., 2013 SAG 30.81A Sydney et al., 2010 However No report of pH effects on B. braunii in continuous culture

2. Continuous culture setup 7th Asia Pacific Biotech Congress 9

10 2. Continuous culture setup : Methodology 7th Asia Pacific Biotech Congress Continuous cultures  homogenous datasets  more reproducible and reliable results (Hoskisson et al., 2005 ) Photobioreactor

11 7th Asia Pacific Biotech Congress Strain  SAG pH  Medium  AF-6 Chemostat  at constant dilution rate D= 0.2 d Continuous culture setup : Strain and parameters pH ±0.1 U pH

3. Results and discussion 7th Asia Pacific Biotech Congress 12

13 7th Asia Pacific Biotech Congress Biomass productivity relatively unaffected by pH between 6,0 and 8,0 (except at pH 5.5) Crude hydrocarbon significantly increased from pH 7.5 to Results and discussion : Biomass and crude hydrocarbon production AF-6 culture medium Dilution rate = 0.2 d -1 T = 23 ±1 °C

14 7th Asia Pacific Biotech Congress 3. Results and discussion : NO 3 - balance analysis in each steady states AF mg L -1 Undetectable  NO 3 - limitation N-rich AF mg L -1 >10 mg L -1  NO 3 - No-limitation Experiments: Condition : N limitation & No-limitation pH: 6.5 & 7.5 S in [NO 3 - ] in S out [NO 3 - ] out Ion Chromatography

15 7th Asia Pacific Biotech Congress Purified hydrocarbon productivity at pH 6.5 higher than at pH Results and discussion : Purified hydrocarbon at pH 6.5 & 7.5 under two NO 3 - conditions Purified hydrocarbon productivity increased under N-sufficient conditions Biomass Hydrocarbon

16 7th Asia Pacific Biotech Congress Hirose et al., Results and discussion : Microscopy and fluorescence observations More hydrocarbon excreted from lipid bodies to matrix at pH 6.5 Bar : 10µm More hydrocarbon accumulated in lipid bodies at pH 7.5 pH 6.5 pH 7.5 Hydrocarbon excretion process  pH-dependent?

4. Conclusions and perspectives 7th Asia Pacific Biotech Congress 17

18 7th Asia Pacific Biotech Congress 4. Conclusions and perspectives Continuous cultures  efficient method to investigate effects of factors on hydrocarbon productivity No-limitation nitrogen condition  increase biomass and hydrocarbon productivities pH  optimal pH range for hydrocarbon productivity in B. braunii SAG cultures  pH-dependent excretion process?  Extracellular carbonic anhydrase activity ? inorganic carbon assimilation  Strain or race-dependent? further study on race B in collaboration with Tsukuba University

7th Asia Pacific Biotech Congress 19 Program supported by CAER (alternative fuel for aeronautics), Nantes University, CSC (N° ) JIN et al., 2015 submitted to Process Biochemistry

7th Asia Pacific Biotech Congress 20

7th Asia Pacific Biotech Congress21 Wu et al., 2007 Nakazawa et al., 2012 Aurantiochytrium sp. (Labyrinthulomycetes) Vibrio furnissii (Bacteria)

7th Asia Pacific Biotech Congress22 Sydney et al., 2010

23 7th Asia Pacific Biotech Congress Oil Kinematic viscosity (mm 2 s -1 ) Density (kg m -3 ) Cetane number Acid number (mg KOH g -1 ) HHV (MJ Kg -1 ) Reference B. braunii oil Ashokkumar et al., 2014 Other microalgae oil * Mallick et al., 2012 Diesel fuel < Amin, 2009 Jet fuel Cramer et al., 2009 *, Oils as biodiesel from Chlorella vulgaris; N/A, no data available. B. braunii oil is suitable for alternative fuels Comparison of different fuels