AP Biology: Thursday, 11/9/11 Lab 5: Measuring Rates of Cell Respiration Homework: Due Tuesday: Finish the pre-lab questions on page 3. Answer any analysis questions that you can (but save graph for class) Start PS 9 Do Now: Get a lab preview worksheet and answer #1-4 Goal for Today: Design and carry out an experiment to measure rates of cell respiration

AP Biology: Wednesday, 11/16/11 Cellular Respiration Homework: Work on PS 9 (due Friday) Do Now: On an index card: What is one thing you learned how to do in Excel yesterday that you didn’t know before? Describe how to do this. What is one question you still have about manipulating data in Excel? In what ways was the cold-temperature experiment yesterday sloppy? How could it have been improved?

Lecture: Cellular Energetics and Cell Respiration Goals: By the end of class you will be able to: Define oxidation and reduction and apply it to the cell respiration chemical equation Describe the role of NADH and FADH2 in cell respiration (and the role of all the molecules in the chemical equation) Describe how the 3 stages of cell respiration transfer energy and electrons from glucose in order to make ATP

Cellular Respiration: An Overview Overall purpose: Release energy from food in the form of ATP for cells to use Where it happens: In the mitochondria of all eukaryotic cells In aerobic bacteria Summary equation: C6H12O6 + 6O2  6CO2 + 6H2O + energy (ATP + heat) glucose + oxygen  carbon dioxide + water + energy

Cell Respiration: A “Redox” Reaction LEO says GER oxidation – the loss of electrons reduction – the gain of electrons e- reduction C6H12O6 + 6O2  6CO2 + 6H2O oxidation

Important Molecules in Cell Respiration Glucose – provides energy and electrons Oxygen – final electron acceptor Carbon dioxide – low-energy waste product from glucose oxidation Water – low-energy waste product from oxygen reduction NADH and FADH2 – intermediate electron carriers Transfer electrons between stages of cell resp.

Cristae Outer membrane Matrix Inner membrane Inter-membrane space (folds in inner membrane) Outer membrane Matrix Inner membrane Inter-membrane space

AP Biology: Thursday, 11/17/11 Cellular Respiration Details Homework: Quiz tomorrow – you can bring one page (one side only) of notes. Typed or hand-written, but on your own paper (not any handout I’ve given you) PS 9 due Monday Do Now: (in your mind or in your notebook) What’s the difference between oxidation and reduction? What do NADH and FADH2 do in cell respiration? What do you think “glycolysis” means?

Lecture: Cell Respiration Details Goals: By the end of class you will be able to: Describe how the 3 stages of cell respiration transfer energy and electrons from glucose in order to make ATP Explain how cell respiration can happen in the absence of glucose, and in the absence of oxygen

Glycolysis: splitting glucose glucose (6 carbons) 2 pyruvate (3 carbons each) P to ETS

Citric Acid Cycle (aka Krebs cycle) intermediate step

Oxidative Phosphorylation (ETS and Chemiosmosis)

Inspirational Video… Then do the first side of the worksheet

Cell Respiration without oxygen: Anaerobic Respiration (Fermentation)

Cell Respiration without oxygen: Anaerobic Respiration (Fermentation)

Two Types of Fermentation: both regenerate NAD+ so that glycolysis can continue ALCOHOL FERMENTATION in yeast cells and some bacteria produces ethyl alcohol and CO2 LACTIC ACID FERMENTATION in vertebrate muscle cells and some bacteria produces lactic acid

AP Biology: Friday, 11/18/11 Cellular Respiration Homework: Problem Set 9 due Monday Do Now: How do you think cell respiration can happen if a cell runs out of glucose? Today’s Agenda: Cell Respiration Wrap-Up Quiz

Cell Respiration without glucose!

Total ATP Production

Total ATP Production

Quiz Work on your own for 20 minutes Use your 1 page of notes Partner Check-in for last 10 minutes Confer with an assigned partner to discuss/check your answers You may not use your notes for this part

Quiz Partners Adel / Imad Talia / Fatima Christina / Djinnie Drishti / Klara Poppy / Rishab Nehemie / Kiraleah Kimberly / Nicole Jasmine / Aaron