Does Treatment With Radio-labeled Antibodies Help Treat Melanoma Tumors? By.David Perez Site: Albert Einstein College Of Nuclear Medicine Mentor: Dr. E. Dadachova This presentation will probably involve audience discussion, which will create action items. Use PowerPoint to keep track of these action items during your presentation In Slide Show, click on the right mouse button Select “Meeting Minder” Select the “Action Items” tab Type in action items as they come up Click OK to dismiss this box This will automatically create an Action Item slide at the end of your presentation with your points entered.

What is Melanoma  Melanoma is the most severe type of skin cancer  It affects an estimated 100,000 people worldwide per year.  It causes tumors in the body.  Melanoma is fatal with 94% of people having a 8.5 month survival rate,while the remaining 6% have a 5 year average survival rate.

Nude Mice  Nude mice are a genetic mutant that do not have thymus gland.  Because of this they have a very weak immune system,and have a low number of T Cells.  Nude mice do not grow hair at any point in their lifetime.  They are used in research because their bodies do not reject any type of tissue or tumor grafts given to them.

How Both Are Related In this Experiment::  Nude mice are injected with human Melanoma cells to provoke tumors in them.  The mice are later treated with antibodies labeled with 188-Re (Rhenium) in hopes of getting rid of the tumors caused by the illness.

The Experiment To Start The Experiment: The mice were weighed and blood samples were taken from them. Blood cells (leukocytes and platelets) in the samples were counted and the numbers were recorded. Human Melanoma cells were injected into the mice in order to induce tumors in them. The mice were later treated with radio labeled anti bodies to see how it would affect the tumors. Mice were weighed again and more blood samples were taken from them. Blood cell numbers were counted and recorded again.

The Experiment Cont. In The Past Week: The mice were weighed and more blood samples were taken from them. The blood samples which would be used to count leukocytes (White Blood Cells) were mixed with 2% acetic acid so that only leukocytes could be seen,while those intended for counting platelets were mixed with Ammonium Oxalate so that only platelets could be seen. The number of leukocytes (White Blood Cells) and platelets in the samples were counted using a hemocytometer, and compared to the numbers recorded from the previous two times.

Desired Results In General The Desired Results Are :  To Decrease the size of the tumors in the mice.  To Slow down the growth of the tumors,or stop their growth altogether.

Materials Used  Cages-> To hold mice  Pipettes-> Pick up liquid in Mkl(Micro liter) measurement  Pipette Tips-> The piece placed at the tip of the pipette that actually holds the liquid.  Skin Caliper-> Used to measure the size of the tumors.  Latex Gloves-> Hand Protection  Scalpels-> To cut the mice so that blood can be drawn from them.  Microscope-> Used to view cells  Hemocytometer->Slide used to view and count cells under a microscope.  2% Acetic Acid->Used to kill all other organisms in a blood sample besides Leukocytes.  Ammonium Oxalate-> Used to kill all other cells in a blood sample besides Platelets.

References  Dead Cells In Melanoma Tumors Provide Abundant Antigen For Targeted Delivery Of Ionizing Radiation By A mAb To Melanin By. Dr.Ekaterina Dadachova,Joshua D. Nosanchuk,Li Shi,Andrew D. Schweitzer,Annie Frenkel, Jerome S. Nosanchuk, and Arturo Casadevall  Dr. E. Dadachova  Dr. K. Revskaya

Acknowledgements I would like to thank: -Dr. E. Dadachova -Dr. K. Revskaya -Dr. Sat Bhattacharya -Harlem Children Society

Thank You!! ^_^