A TOMIC - ABSORPTION SPECTROSCOPY. Atomic absorption spectroscopy (AAS) is a technique for determining the concentration of a particular metal element.

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Presentation transcript:

A TOMIC - ABSORPTION SPECTROSCOPY

Atomic absorption spectroscopy (AAS) is a technique for determining the concentration of a particular metal element (e.g.Fe, Cu, Al, Pb, Ca, Zn)in a sample ( water, medicine, food). Atomic absorption spectroscopy can be used to analyze the concentration of over 70 different metals in a solution.element Introduction

T ECHNIQUE Atomic-absorption (AA) spectroscopy uses the absorption of light to measure the concentration of gas-phase atoms. Since samples are usually liquids or solids, the analyte atoms must be vaporized or atomized in a flame. absorption

S TEPS The steps are involved in turning a liquid sample into an atomic gas: Desolvation – the liquid solvent is evaporated, and the dry sample remains.solventevaporated Vaporisation or Volatilization – the solid sample vaporises to a gas.

Instrumentation

The light that is focused into the flame is produced by a hollow cathode lamp.hollow cathode lamp Light Source: Hollow Cathode Lamp

The type of hollow cathode tube depends on the metal being analyzed. For analyzing the concentration of copper, a copper cathode tube would be used.

Sample solutions are usually aspirated and entering to the flame  excitation by heat of flame  absorb light from hallow cathode lamb  to go from ground state E  to higher energy level E1  monochromator  detector measuring the reduction of intensity of light refer to conc of sample. Each Element have specific E that will absorb a specific wavelength of light.

a graphite-furnace atomic-absorption spectrometer: a flame atomic-absorption spectrometer

Which is set to isolate the radiation at the specified wavelength and travels into the detector. Monochromator

D ETECTOR Photo multiplier tube Extremely sensitive Can detect single photons Measures the intensity of the beam of light. When some of the light is absorbed by metal, the beam's intensity is reduced. The detector records that reduction as absorption. That absorption is shown on output device by the data systemoutput device

Schematic of an atomic-absorption experiment