Membrane Function
Passive Transport Concentration Gradient Concentration Gradient A difference in concentrations across the plasma membrane
Simple Diffusion Particles diffuse through phospholipid bilayer Particles diffuse through phospholipid bilayer
Facilitated Diffusion Translocating (Carrier) Proteins Translocating (Carrier) Proteins Bind particle Change shape Force through membrane
Facilitated Diffusion Channel Proteins Channel Proteins Passage through proteins Gated - open in response to stimulus Membrane proteins exhibit… Membrane proteins exhibit… Specificity Saturation Inhibition
Osmosis Diffusion and fac. diffusion of water Diffusion and fac. diffusion of water
Osmosis Continues until equilibrium reached Continues until equilibrium reached OR until another force resists osmosis OR until another force resists osmosis p = Pressure Potential s = Solute Potential
Osmosis Water Potential ( ) Water Potential ( ) Water moves from high to low Depends on s and p s = - i C R T Solution s at 20C Pure Water 1.0M Sucrose 0.2M NaCl Ionization constant (units/molecule) Molar Conc. Pressure Constant (0.083) Temperature (in K; C)
Active Transport Membrane proteins use ATP to increase conc gradient Membrane proteins use ATP to increase conc gradient
Active Transport Sodium-Potassium Pump Sodium-Potassium Pump
Electrochemical Gradient Difference in charges and concentrations across a membrane Difference in charges and concentrations across a membrane Produced by electrogenic pumps Produced by electrogenic pumps Serves as potential energy that can be tapped later Serves as potential energy that can be tapped later
Exocytosis & Endocytosis
Transport Review 1. What types of particles have the toughest time getting through a phospholipid bilayer? 2. What similarities exist between transport proteins and enzymes? 3. What’s the difference between carrier proteins and channel proteins? 4. A certain membrane is impermeable to ions and can withstand 2 bars of pressure before rupture. At 27 o C, how high can the salt concentration be inside the cell before rupturing in a distilled water environment?