ProStar 335 Detector Wednesday, 26 April 2017 Petro van Poppel – Field Support Specialist Wednesday, 26 April 2017

Overview Production Tests with 0x0 flow cell Introduction to PS 335 Detector Dual Array option Poly Verify Software Flow cell Communications Poly Verify Production Tests with 0x0 flow cell Wavescan (Settings Tab) (Input/Output Tab) (EEPROM) (COMMS Tab) (Calibration Tab) PS 335 Shipping Test Problems

ProStar 335 Mechanical Optical layout

ProStar 335 Ray Trace Layout

335 Optics

335 Ray Trace

335 Single and Dual Array Single Array 1024 photodiode sample array 2 photodiode references (250nm and 500 nm) No extended range spectra Uses only 9x0 and 4x0 flow cells Microbore to Analytical applications Dual Array 1024 photodiode sample and reference array Extended range spectra (up to 70-100 AU) Used for analytical to prep apps

Flow Cell Ray Beam General design of the 9x0 flow cell

Flow Cell Specs Flow Cell Prep (9 x 1) Super Prep (4 x 0.15) Analytical (9 x 0) Micro (4 x 0 ) Pressure rating 1000 psi Flow Cell Volume 30 µl 145 µl 15 µl 1.5 µl Material (non optical) Ti & PEEK Noise Performance 200 ml/min 10-500ml 1x10-4AU 800 ml/min 0.80-10 L 40 ml/min 0.1 µl-100 ml 5x10-3AU 1 ml/min 0-20 ml 7.5x10-3AU

Communications TCP/IP communications for Detector: Static or Dynamic Networked or standalone Firewall Access on Network, OS or PC

PS 335 PolyVerify Software

PS 335 PolyDiagnostics Ctrl-Shift-F10 to enable Diagnostics side Ctrl-Shift-F9 to hide Diagnostics side

335 PolyVerify Test Certificate Test Certificate provided by the factory for each 335 detector

PS 335 PolyVerify Tests

PS 335 Noise Test

PS 335 Wavelength Accuracy test Run with a 4 nm slit

PS 335 SBW Accuracy Test Wavelength Range of 190 to 950 nm

PS 335 Detector Linearity Test pump head pressure of at least 100 atm using a back pressure restrictor of 40psi .

PS 335 Wavescan

PS 335 Wavescan Results This wavescan seems to have a rather large difference between the sample and reference paths (long and short paths). Possible reasons for this are bad calibration, dirty flow cell, air bubbles in flow cell, foggy optics.

PS 335 Contributions from each Lamp

PS 335 PolyDiagnostics Mode

PS 335 Input/Output Mode

PS 335 Main PWB Power Supply LED’s C A: + 24 V power Supply B: + 9V and – 9V Power Supply C: + 5 V Power Supply Front of the Unit

PS 335 Identifying the Array Type Knowing the Array type is necessary when running the Input/Output test diagnostics

PS 335 Dual Array P8 disconnect P8 connection

PS 335 PolyDiagnostics EEPROM

PS 335 PolyDiagnostics COMMS

PS 335 PolyDiagnostics Calibration Tab

Caffeine Shipping Test PS 335 Caffeine Shipping Test: Test made up of 1 components: Loop injection using 25 µg/ml caffeine Test is conducted using Galaxie Software

PS 325/335 Caffeine Loop Injection Test Test Calculates: Peak Height Peak Area Noise S/N

Common Problems PS 335 Error Messages Table can be found: High Noise Lamp on, not ready SIG_CC_SLIT_PEAK_NOT_FOUND SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED 9555 LMP_CC_D2_FILAMENT_FAILURE Foggy Optics PS 335 Error Messages Table can be found: PS 335 Service Manual Dealernet

Foggy Optics What is Foggy Optics?

What is Foggy Optics? Three types of Foggy optics White powdery coating Rust/brown spot burn Speckled mirror (delamination)

What is Foggy Optics? Diagnosing Foggy Optics

Diagnosing Foggy Optics Run a “wavescan” test, When bad Open the optics and inspect.

Wavescan test results Intensity for the 254 nm line Currently is 10 – 50 K counts

False positive New D2 vs Aged D2 Intensities

What is Foggy Optics? Diagnosing Foggy Optics Treating Foggy optics

0603 Project (Foggy Optics) Proposed Fixes and their status: White powder fogging: due to leaky flow cells and customer sample depositing on the mirrors New Flow cells & fittings available for all cells except 4 x 0.15 Brown powder fogging: due to UV attack on some of the foam parts close to the UV source. Eliminating as much of the foam as possible and shielding the rest. Mirror delamination: due to UV attack on the thin Al coating of the mirrors Eliminated by putting a NiCr coating on the mirrors to strengthen the Al coating.

Opening and cleaning the optics

Cleaning 325 optics Clean area in circle Do not adjust!!

Wavelength calibration data must be entered into the 335 EEPROM Replacing Optics Wavelength calibration data must be entered into the 335 EEPROM

Replacing Optics This label is attached to the top of each optics module, the shipping box and the test document

Summary Single and Dual Array Optical path is open Flow cell is dual path Diagnostics Tests, like wavescan Shipping Test Main Problems (Foggy Optics)