Optimization of T-Cell Trapping in a Microfluidic Device Group #19 Jeff Chamberlain Matt Houston Eric Kim.

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Presentation transcript:

Optimization of T-Cell Trapping in a Microfluidic Device Group #19 Jeff Chamberlain Matt Houston Eric Kim

MEMS- MicroElectroMechanical Systems Batch Fabrication Processes Cell Traps –High-throughput experimentation –Complex biochemical analysis –Single cell analysis –Reagent conservation –Quick environmental changes

Cell Trapping Basics Reagent #1 Reagent #2 Cells / Media Trap arrays:

Our Project Maximize trap efficiency by improving upon current trap designs. –Trap Efficiency : maximized number of traps with 1 cell/trap

Cell Viability Results Flow Rate = 100 nl/min Flow Rate = 250 nl/min

SolidWorks ® Rendering of a Single Well

Picture of Well Array Square or rectangular shaped well of any depth Thousands of mirrored wells in one etching Front Surface Mirrors with high reflectivity Nearly orthogonal views of specimen 200 um

Background & Motivation Three Dimensional Image Information May Be Important for Biological Studies Chemotaxis Developmental Biology Cellular Division Pinocytic Loading Volumetric Measurements

Our System is Constructed From Silicon Wafer

Methods: Fabrication Silicon Wafer Silicon Wafer Grow SiO 2 Spin Coat Mask Layer Pattern with Photolithography Etch with HF Etch with HF Remove Photoresist Etch with KOH Coat with Platinum or Aluminum Cutaway View

SolidWorks ® Rendering of a Single Well

Results: Dictyostilium Extrusion A B C A B C Primary Image Reflection Reflection Reflection of the Reflection Reflection Objective

Coupling Microfluidics With the Pyramidal Wells Si Wafer Cross Section of One Well PDMS Flow PDMS Glass

Pyramidal Well Dimensions depth_min = cell_radius outside_dim = cell_radius Assuming: 0.5 base = 1.2 cell_radius

Micromirror Well Dimensions Cell TypeCell Dimensions (um)Minimum Required Depth (um) Outside Dimensions for Min Depth (um) T-cells5 diameter Jurkats10 diameter Dendritic Cells10-20 diameter Dicti10-20 diameter Myocyte15 by by

Future Directions Single Cell Design –Desire single cell per well for optimal imaging –Possibly controllably coupled with another As opposed to…

Future Directions ? Single Cell Design –Desire single cell per well for optimal imaging –Possibly controllably coupled with another

Future Directions Flow Modeling –Design a more efficient trapping system –Use flow data to design trap design that will keep cell in well ?

Future Directions PDMS-mirror bonding –Questions to whether the PDMS will bond to the mirror array –Use SU-80 and glass cover slip with a PDMS external flow system?