Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university

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Presentation transcript:

Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university

Exemplary fluorescence images of one animal after injection 2 μmol/kg TSC (tetrasulfonated carbocyanine) intravenously into F9-teratocarcinoma bearing mice Christin Perlitz, etc //Journal of Fluorescence, Vol. 15, No. 3, May 2005

Enzyme Cells Monomer photosensitizer Enzyme Dimer Monomer photosensitizer fixed moving

Maryakhina V.S., Gunkov V.V. Computer research and modeling V.5.- №5.- p Dynamics of bengal rose fluorescence in model solution of collagenase with mammary cells

Concentration changes of photosensitizer molecules at immobilization process received from two models

Changing of fluorescence intensivity at 560 nm Enzyme 560 nm 545 nm C col =0.1 mg/ml C col =0.5 mg/ml Maryakhina V.S., Gunkov V.V. Computer research and modeling V.5.- №5.- p

Comparison of experimental and theoretical results The normalized experimental (1,2) and theoretical (3,4) fluorescence spectra of erythrosine in collagenase solution 0.1 mkg/ml after addition of dye aliquot with initial concentration: 1, mМ; 2,4- 1 mМ. Maryakhina V.S., Gunkov V.V. Computer research and modeling V.5.- №5.- p

Maryakhina V.S., Letuta S.N.// Laser physics V p eosine bengal rose М М 4- health mouse 3- cancer 1 cm 2- cancer 2 cm 1- cancer 3 cm bengal roseeosine

Collagenase (powder) + eosine М + eosine М Fluorescent microscopy results Maryakhina V.S.// Proc.. of SPIE V. 8699

w blood t1t1 t2t2 t3t3 t1t1 t2t2 t3t3

For biological liquid flow (blood, for example) - differential equation of convective diffusion (moving medium) a few phases w w, w p, w cell - rate of water, peptide and cell phases assumptions - w w = w p = w cell - movable coordinates

Initial and bordering conditions 0.5 mm d vessel = 0.5 cm C w (r,0) = Gauss destribution D w = cm 2 /s D p = cm 2 /s D cell = cm 2 /s C p (r,0) = 2 C cell (r,0)

600 s Concentration of injected compound Concentration of free cellsConcentration of free peptides

Thank you for your attention This work is partially supported by grant of RFBR № mol_a