Multiparametric flow cytometry of normal human bone marrow:

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Presentation transcript:

Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies GEIL-GTLLF 2008 Part one : Leukocyte subsets A colour code is applied trhoughout this atlas: Granulocytes in red Monocytes in green Lymphocytes in purple All other cells, in a region of maturation defined by the exclusion of mature cell types and thus dubbed « bermudes » in cyan I.1

Combinations Tube 1 : CD11b/CD14/CD16/CD45 Tube 7 : HLADR/CD117/CD34/CD45 CD11b/CD14 p. I-5 HLADR/CD117 p. I.29 CD11b/CD16 p. I-6 DR/CD34 p. I.30 CD14/CD16 p. I-7 CD117/CD34 p. I.31 Tube 2 :CD11b/CD117/CD34/CD45 Tube 8 : CD34/CD56/CD19/CD45 CD11b/CD117 p. I-9 CD34/CD56 p. I.33 CD11b/CD34 p. I-10 CD34/CD19 p. I.34 CD117/CD34 p. I-11 CD56/CD19 p. I.35 Tube 3 :CD36/CD71/CD11c/CD45 Tube 9 : CD64/CD24/CD10/CD45 CD36/CD71 p. I-13 CD64/CD24 p. I.37 CD36/CD11c p. I-14 CD64/CD10 p. I.38 CD71/CD11c p. I-15 CD24/CD10 p. I.39 Tube 4 : CD34/CD13/CD33/CD45 Tube 10 : CD7/CD117/CD34/CD45 CD34/CD13 p. I-17 CD7/CD117 p. I.41 CD34/CD33 p. I.18 CD7/CD34 p. I.42 CD13/CD33 p. I.19 CD117/CD34 p. I.43 Tube 5 : CD38/HLADR/CD34/CD45 Tube 11 : CD22/CD10/CD20/CD45 CD38/HLADR p. I.21 CD22/CD10 p. I.45 CD38/CD34 p. I.22 CD22/CD20 p. I.46 HLADR/CD34 p. I.23 CD10/CD20 p. I.47 Tube 6 : CD38/CD10/CD19/CD45 Tube 12 : CD4/CD2/CD56/CD45 CD38/CD10 p. I.25 CD4/CD2 p. I.49 CD38/CD19 p. I.26 CD4/CD56 p. I.50 CD10/CD19 p. I.27 CD2/CD56 p. I.51 I.2

Leukocyte subsets display FSC SSC CD45 SSC FLx FLy FLx FLy FLx FLy FLy FLx I.3

CD11b/CD14/CD16/CD45 TUBE 1 I.4 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD11b/CD14 x x CD11b/CD16 x CD14/CD16 I.4

CD11b/CD14 I.5 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD11bFITC SSC-Height CD14PE FLx FLy CD45 SSC FSC

CD11b/CD16 I.6 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD11bFITC SSC-Height CD16PC7 FLx FLy CD45 SSC FSC

CD14/CD16 I.7 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD14PE SSC-Height CD16PC7 FLx FLy CD45 SSC FSC

CD11b/CD117/CD34/CD45 TUBE 2 I.8 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD11b/CD117 x x CD11b/CD34 CD117/CD34 I.8

CD11b/CD117 I.9 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD11bFITC SSC-Height CD117PE FLx FLy CD45 SSC FSC

CD11b/CD34 I.10 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD11bFITC SSC-Height CD34PC5 FLx FLy CD45 SSC FSC

CD117/CD34 I.11 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD117PE SSC-Height CD34PC5 FLx FLy CD45 SSC FSC

CD36/CD71/CD11c/CD45 TUBE 3 I.12 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD 36/CD71 x CD36/CD11c x CD71/CD11c I.12

CD36/CD71 I.13 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD36FITC SSC-Height CD71PE FLx FLy CD45 SSC FSC

CD36/CD11c I.14 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD36FITC SSC-Height CD11cPC5 FLx FLy CD45 SSC FSC

CD71/CD11c I.15 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD71PE SSC-Height CD11cPC5 FLx FLy CD45 SSC FSC

CD34/CD13/CD33/CD45 TUBE 4 I.16 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD34/CD13 x x CD34/CD33 CD13/CD33 I.16

CD34/CD13 I.17 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD34FITC SSC-Height CD13PE FLx FLy CD45 SSC FSC

CD34/CD33 I.18 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD34FITC SSC-Height CD33APC FLx FLy CD45 SSC FSC

CD13/CD33 I.19 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD13PE SSC-Height CD33APC FLx FLy CD45 SSC FSC

CD38/HLADR/CD34/CD45 TUBE 5 I.20 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD38/DR x x CD38/CD34 DR/CD34 I.20

CD38/HLADR I.21 SSC FSC CD45 FLy FLx CD45ECD FSC-Height CD38FITC SSC-Height HLADRPE FLx FLy CD45 SSC FSC

CD38/CD34 I.22 SSC FSC CD45 FLy FLx CD45ECD FSC-Height CD38FITC SSC-Height CD34PC5 FLx FLy CD45 SSC FSC

HLADR/CD34 I.23 SSC FSC CD45 FLy FLx CD45ECD FSC-Height HLADRPE SSC-Height CD34PC5 FLx FLy CD45 SSC FSC

CD38/CD10/CD19/CD45 TUBE 6 I.24 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD38/CD10 x x CD38/CD19 x CD10/CD19 I.24

CD38/CD10 I.25 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD38FITC SSC-Height CD10PE FLx FLy CD45 SSC FSC

CD38/CD19 I.26 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD38FITC SSC-Height CD19Cy5 FLx FLy CD45 SSC FSC

CD19/CD10 I.27 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD10PE SSC-Height CD19Cy5 FLx FLy CD45 SSC FSC

HLADR/CD117/CD34/CD45 TUBE 7 I.28 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage DR/CD117 x DR/CD34 x CD117/CD34 x I.28

HLADR/CD117 I.29 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height HLADRFITC SSC-Height CD117PE FLx FLy CD45 SSC FSC

HLADR/CD34 I.30 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height HLADRFITC SSC-Height CD34Cy5 FLx FLy CD45 SSC FSC

CD117/CD34 I.31 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD117PE SSC-Height CD34Cy5 FLx FLy CD45 SSC FSC

CD34/CD56/CD19/CD45 TUBE 8 I.32 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD34/CD56 x x CD34/CD19 x CD56/CD19 I.32

CD34/CD56 I.33 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD34FITC SSC-Height CD56PE FLx FLy CD45 SSC FSC

CD34/CD19 I.34 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD34FITC SSC-Height CD19PC7 FLx FLy CD45 SSC FSC

CD56/CD19 I.35 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD56PE SSC-Height CD19PC7 FLx FLy CD45 SSC FSC

CD64/CD24/CD10/CD45 TUBE 9 I.36 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD64/CD24 x x CD64/CD10 x CD24CD10 I.36

CD64/CD24 I.37 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD64FITC SSC-Height CD24PE FLx FLy CD45 SSC FSC

CD64/CD10 I.38 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD64FITC SSC-Height CD10Cy5 FLx FLy CD45 SSC FSC

CD24/CD10 I.39 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD24PE SSC-Height CD10Cy5 FLx FLy CD45 SSC FSC

CD7/CD117/CD34/CD45 TUBE 10 I.40 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD7/CD117 x x CD7/CD34 CD117/CD34 x I.40

CD7/CD117 I.41 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD7FITC SSC-Height CD117PE FLx FLy CD45 SSC FSC

CD7/CD34 I.42 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD7FITC SSC-Height CD34Cy5 FLx FLy CD45 SSC FSC

CD117/CD34 I.43 SSC FSC CD45 FLy FLx CD45Cy7 FSC-Height CD117PE SSC-Height CD34Cy5 FLx FLy CD45 SSC FSC

CD22/CD10/CD20/CD45 TUBE 11 I.44 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD22/CD10 x CD22/CD20 x CD10/CD20 I.44

CD22/CD10 I.45 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD22FITC SSC-Height CD10PE FLx FLy CD45 SSC FSC

CD22/CD20 I.46 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD22FITC SSC-Height CD20PC5 FLx FLy CD45 SSC FSC

CD10/CD20 I.47 SSC FSC CD45 FLy FLx CD45PC7 FSC-Height CD10PE SSC-Height CD20PC5 FLx FLy CD45 SSC FSC

CD4/CD2/CD56/CD45 TUBE 12 I.48 Granulocytes Monocytes Lymphocytes Granulocytes Monocytes Lymphocytes Bermudes B T NK GM progenitors B lineage CD4/CD2 x CD4/CD56 CD2/CD56 x I.48

CD4/CD2 I.49 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD4FITC SSC-Height CD2PE FLx FLy CD45 SSC FSC

CD4/CD56 I.50 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD4FITC SSC-Height CD56PC7 FLx FLy CD45 SSC FSC CD4/CD56 I.50

CD2/CD56 I.51 SSC FSC CD45 FLy FLx CD45APC FSC-Height CD2PE SSC-Height CD56PC7 FLx FLy CD45 SSC FSC

Published on ELN website July 2008 Contributors Christine Arnoulet Marie Christine Béné Françoise Durrieu Jean Feuillard Chantal Fossat Bernard Husson Hélène Jouault Francis Lacombe Marc Maynadié Coordination Francis Lacombe & Marie Christine Béné