Microscopy Proper Handling And Use of Microscopes.

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Presentation transcript:

Microscopy Proper Handling And Use of Microscopes

Proper Care and Handling of the Microscope is Important!  ALWAYS carry a microscope with 2 hands. One under the base and one holding the arm of the microscope.

1.Orient the microscope so the stage is facing you, not the microscope arm.  Turn/twist the eyepiece around so it is angled toward you.  The focus knobs will be on your right. 2.Turn on the light. 3.Rotate the nosepiece to put the scanning power objective lens in place, centered over the hole in the stage.  The shortest lens; the lowest magnification power.  Total magnification = eyepiece lens x objective lens

4.Place the glass slide under the stage clips, center the specimen.  Visually place the “specimen” on the slide over the small circle on the stage where light shines through. 5.Look through the eyepiece. Use the coarse adjustment knob to move the stage and bring the “specimen” into focus.  The diaphragm under the stage adjusts the amount of light coming through.

6.Turn the nosepiece to center the low power objective over the slide. It must “click” into place. 7.Use the fine focus adjustment knob to bring the specimen into focus.  If directed, when you go to high power, only use the fine focus adjustment!  If the specimen goes out of focus, back up to low power or even scanning power and start again.

Making a “wet mount” 1-2 drops of water or stain put over the specimen. Put coverslip down at a 45° angle and slowly lay it down. Wick away any excess liquid.