The Microscope The Unseen World

The Penny

The Penny VDB Victor D. Brenner

Frank Gasparro

Is an instrument which provides an enlarged image of an object. The Microscope!! Is an instrument which provides an enlarged image of an object.

History Anton Von Leeuwenhoek 1632 First to observe living bacteria & drew them. Also looked at sperm & blood

Ash Tree cross- section The Universality of Sex (sperm) Three shapes of bacteria

Robert Hooke (1665) Examines cork. First to use the term “cell” referring to the many little boxes in cork.

Most commonly used microscope Light Microscopes The type we use in our labs Most commonly used microscope Uses light and lenses to magnify & view the specimen Has two sets of lenses – Ocular (eye piece) & Objective (near the object being viewed) Total magnification on our scopes = 40 – 1000 times Total magnification = Ocular (10X) x Objective (40X Low: 4 x 10 = 40 Med: 10 x 10 = 100 High: 40 x 10 = 400

Ocular – Eye piece (magnifies 10X) Neck – Supports the eyepiece. Objectives – 4 – 100x magnification Arm – Supports neck and objectives. Carry by this Stage and clips – Holds slides in place Adjustments – Coarse & Fine. Focuses image Diaphragm – Controls the amount of light coming through the stage Light – Electric light source Base – Bottom of scope. One hand goes underneath

The Diaphragm Use the Diaphragm to adjust the amount of light

Focusing Use the Adjustment knobs to focus the image Coarse adjustment brings the image into near focus Fine adjustment (smaller knob) brings it into fine focus. Only use this one when on high power

Rules for using the Microscope Use only the assigned microscope Carry & place the scope properly (6cm from edge of table) Do not let the cords dangle or get into the sinks Clean lens only with lens paper. NO FINGERS! Start on low (4x) power when you start your observations Always focus (move the stage) away from the slide Use the coarse adjustment first then the fine adjustment Be careful when switching to high (40x) power to se that there is enough clearance between the objective and the slide Do not use the coarse adjustment knob on high (40x) power When you are done with the scope, turn off the light switch Return the low power objective (4X) into place and raise the stage all the way up. Always put scope away with cord wrapped around it, cover on & the low power objective in place Put scopes away with the numbers facing out into the proper slot Clean and dry all slides and cover slips before putting them away

Compound Microscope images Diatom Amoeba Paramecium Hydra budding Daphnia Euglena

Electron Microscopes Uses electromagnets and streams of electrons to view a specimen 200,000 – 1,000,000x magnification Animal cell Plant cell

Transmission Electron Microscope (TEM) 1931 Image is seen on a computer screen Specimen must be thinly sliced and coated with metal. Gives a 2D image of specimen Specimen must be dead

Herpes simplex viruses Staphylococcus aureus These have all had color added to them!!! E. coli bacteria

Scanning Electron Microscope (SEM) – 1935 Gives a 3D image Hookworm mouth!!!!!!

Images Diatom Weevil Tick Radiolarian

Making a Wet Mount Slide 1. Get a thin slice/piece of whatever your specimen is. 2. Place ONE drop of water directly over the specimen. 3. Place the coverslip at a 45 degree angle (approximately), with one edge touching the water drop, and let go.

The Letter “e” 40x 400x

Threads 100x

Beware of Air Bubbles!!