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2 Last Workshop… ImageJ basics –Image manipulation –Setting scale –Making measurements –Counting particles

3 Today’s Plan… Advanced Image Analysis –Working with “stacks” Where to get images –Finding existing images –Creating your own images Online exercises –SimpleScience.org

4 Stacks A collection of multiple images packaged together into a single computer file Think of a deck of cards –X & Y dimensions are spatial –Z dimension can be: Spatial (depth) Temporal (time - video) Spectral (color)

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53 CHANNEL DEPTH TIME

54 Where To Get Images… Use somebody else’s Create your own

55 Online Image Archives zation/collections/imagearchives.htmlhttp://serc.carleton.edu/NAGTWorkshops/visuali zation/collections/imagearchives.html

56 Creating Your Own Image Stacks Most any camera can be used to create scientifically useful images –Still –Video In most cases, Z dimension will be time

57 Still Cameras Whenever possible, use tripod Digital images have date/time stamps Stacks can be built up manually in ImageJ

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60 Using Video In ImageJ QuickTime movies are opened as stacks –Must have QuickTime 7 installed

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87 Joe Pow Chester F. Carlson Center for Imaging Science Rochester Institute of Technology