Working with rDNA Safety considerations. rDNA constructed outside of a living cell from more than one source capable of replicating in a living cell NIH.

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Presentation transcript:

Working with rDNA Safety considerations

rDNA constructed outside of a living cell from more than one source capable of replicating in a living cell NIH guidelines rDNA encoding insulin sites.thefullwiki.org/05/4/5/3/ png

Risk Assessment: Organism or Vector Consider: host organism –routes of human infection survival outside of lab transfer of genetic material wikipedia/commons/3/32/Es cherichiaColi_NIAID.jpg pedia/commons/4/46/Baculoviru s.jpg rg/wikipedia/commons/a/ a0/Hela_Cells_Image_3 709-PH.jpg ipedia/commons/6/6f/Arabidop sis_thaliana.jpg

Risk Assessment: Nature of DNA Sequence Consider: level of product expression biological stability of product coding for production of toxins coding for antibiotic resistance coding for allergens coding for properties that could make host more infectious biotechnology.com/rootgif/images/diagramm02.jpg

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