Pg. 367
Membrane Protein Movement Tethered to other Membrane proteins Single Protein Tethered to cytoskeleton Pg. 385
Cytosol Nucleus Mitochondria Chloroplasts ER Golgi Endosome Lysosome Transmembrane Secreted
Cytosol Nucleus Mitochondria Chloroplasts ER Golgi Endosome Lysosome Transmembrane Secreted
The Nucleus
Nuclear Structure
Nuclear Lamina
Nuclear Structure
Nuclear Pore Complex
Protein Transport to the Nucleus Nuclear Localization Signal (NLS) -Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val- N C Table 15-1 (page 504)
NLS is Necessary for Nuclear Import Protein with NLS Nucleus Nuclear protein with NLS removed from protein Pg. 504
NLS is Sufficient for Nuclear Import Nucleus Nuclear protein with NLS removed from protein Cytosolic Protein Without NLS With NLS Pg. 504
Nuclear Import Pathway
Pg. 541
Regulated Nuclear Import NLS “masked” Allostery! NLS “exposed” Pg. 542
Cytosol Nucleus Mitochondria Chloroplasts ER Golgi Endosome Lysosome Transmembrane Secreted
ER Import Sequence Table 15-1 (page 504) Recognition Sequence N-Met-Met-Ser-Phe-Val-Ser-Leu-Leu-Leu-Val-Gly-Ile-Ile-Phe-Trp-Ala-Thr- C Hydrolysis of this peptide bond by Signal Peptidase enzyme Table 15-1 (page 504)
N
Membrane “budding” N C N C N C N C N C
Clathrin “triskelion”
Clathrin “Cage”
Dynamin GTP Hydrolysis
Vesicle Fusion with Membrane
v-SNARE/t-SNARE Interaction
Cytosol Outside of cell Membrane fusion step is regulated Secretory vesicle Ca++ Outside of cell
Botulism Toxin Clostridium botulinum Protease
Botulism Toxin t-SNARE (green) Botulism Toxin (tan)
Botulism Toxin Botulism Toxin Protease