Important Lab instrument

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Presentation transcript:

Important Lab instrument Microscope Important Lab instrument

Why use a microscope? To view objects and detail too small to see with human eye. Improves resolution of object List examples of how what you used it for: Blood cell detail rbc, wbc, platelets Bacteria Cocci, rod, bacillus Protozoa Trichomonas Giardia

Types of Microscopes Compound microscope Electron microscope Bacteria, fungi and protozoa Electron microscope Required for viruses Fluorescence Used as a diagnostic tool for immunofluorescence tests

Parts of a microscope Coarse adjustment Fine adjustment Stage 1st step in focusing to change the distance between specimen and lens Fine adjustment To fine tune the picture Used particularly with 100x and oil objective Stage Holds the slide, moved up and down with coarse and fine adjustment

Parts of a microscope Objectives Common objectives are 4x, 10x, 40x, 100x, oil Total magnification is eye piece magnification multiplied times objective you are viewing with 40x objective and 10x eye piece is 400x magnification Oil objective is labeled and is always used with immersion oil Oil increases the resolving power by focusing the light rays

Proper care for microscope Always start with lowest objective to focus Always store with lowest objective locked in place Carry with two hands Cover with dust cover Be sure to clean oil off of oil objective Use fine adjustment with 100x and oil objective

THE COMPONENTS OF THE MICROSCOPE What do you remember?

THE MICROSCOPE EYEPIECE What is it when there is only 1? When there are 2?

THE MICROSCOPE ARM

THE MICROSCOPE BASE

THE MICROSCOPE TUBE

THE MICROSCOPE REVOLVING NOSEPIECE

THE MICROSCOPE OBJECTIVE LENS What are the magnification of the 4 objectives? 4, 10, 40, 100x What magnification is the eye piece? 10x unless otherwise stated What would be the total magnification when viewing an object through the 40x objective? 400x

THE MICROSCOPE MECHANICAL STAGE Why is a mechanical stage better than using clips?

THE MICROSCOPE IRIS DIAPHRAGM What does this do? Control the amount of light

THE MICROSCOPE COARSE ADJUSTMENT KNOB What 2 objectives do you use the coarse adjustment? 4 and 10x

THE MICROSCOPE FINE ADJUSTMENT KNOB What objectives is this used with? All, but mostly 40, 100x

THE MICROSCOPE Light

THE MICROSCOPE ON/OFF SWITCH

CARE OF THE MICROSCOPE Always carry the microscope by the arm and the base and use 2 hands!

CARE OF THE MICROSCOPE Wrap the cord up or around the microscope so it is not hanging down.

CARE OF THE MICROSCOPE Always lower the stage and clean all objectives off Always use a dust cover!