1. The parent DNA molecule is split into two 2. New nucleotides are added to each of the two DNA strands 3. The base pairing rules determine which nucleotide is added  If a T is on the original strand an A is added to the daughter strand  If a C is on the original strand a G is added to the daughter strand.

 DNA has an opposite position to each other; also called “opposite orientation” or antiparallel

 3’ (three-prime)- has three carbons on the nucleotide and an OH (hydroxyl) group  5’ (five-prime)- has five carbons on the nucleotide and an PO 4- (phosphate) group

1. Replication starts on the origins of replication  Origins or Replication- replication begins were proteins attach to the DNA molecule. 2. Replication takes places in both directions  The result: replication bubbles

3. There can be thousands of “bubbles” at one time as DNA replication takes place in eukaryotic organisms 4. DNA polymerases – enzymes that join individual nucleotides to make a new DNA molecule

 5. DNA Polymerase adds nucleotides to the 3’ end of the strand; not the 5’ end.  The result: The daughter strand only grows from 5’ to 3’  Replication Forks- the sites where separation & replication take place  The daughter strand is formed in short pieces that need to be connected  6. DNA Ligase- connects the pieces into a single DNA strand

 7. Lastly; After the new strand is added DNA Polymerase “proofreads” the new strand to make sure each strand is a perfect copy of the old stand