Figure S UV- UV+ Relative expression levels of rhoB mRNA by qRT-PCR *

28S 18S tRNA UV-UV+ : 60J/m2 input Figure S % of actin mRNA in each fraction fractions % of rhoB mRNA levels in each fraction fractions

Figure S3 Relative luciferase activity (Rluc/FLuc) RLuc rhoB 3’UTR RLuc P53 3’UTR Donor 1Donor mockmiR- 19b (2 nM) Figure S4 Relative levels of miR-19b by qRT-PCR Transfection: ***

Figure S U6snRNARNU44RNU U6snRNA RNU44RNU48 Normalization: Relative levels of indicated miRNA by qRT-PCR miR-18amiR U6snRNARNU44RNU U6snRNARNU44RNU48 miR-30amiR-30cmiR-30e miR-21miR-223miR U6snRNARNU44RNU48 U6snRNARNU44RNU48U6snRNARNU44RNU U6snRNA RNU44 RNU48 UV- UV+

Figure S6 Amount of rhoB mRNA in crosslinked IP (qRT-PCR) UV- UV+ IP HuR ** IP Igg1

Figure S7  HuR (3A2) Igg mouse DAPIFITC No UV  HuR (3A2) Igg mouse UVC (60 J/m 2 ) Merge

Tubulin HuR Topoisomerase I UV: Nuc. Cyto kDa 68 hnRNP C1 / C2 CFI Figure S8 b a Amount of rhoB mRNA in IP (qRT-PCR) UV- UV+ IP HuR ** IP Igg1

RhoB actin HuR UV: siHuRsiHuR’siCtrl Figure S9

IP Igg1 IP HuR UV+ Figure S10 Amount of rhoB mRNA in IP (qRT-PCR) Mock miR-19 * * IP Ago2 * * 35

b TUNEL – positive cells (% of total cells) (after UV treatment) Mock miR-24 miR-19b siRhoB Figure S11 a Mock miR-24 miR-19b siRhoB DAPI TUNEL DAPI TUNEL UV-UV+ ** *

Table S1 Log-Fold-Change Log-Odds miR miR miR-33a miR-92b* miR-576-5p miR-520c-3p miR-1-2-as miR miR miR-673-3p UV regulated miRNAs according to the miRNA microarray results

Table S2 Foward R everse 3’UTR rhoB5’GCTCTAGAAAGGTGCTATG AGGGCCG 3' 5’CGGGATCCCGGAGTTGGCAAGA AAGGATCT 3' 3’UTR p535’CAGACTAGTTGACTCAGAC TGACATTCT 3‘ 5’GCTAGATCTTGGCAGCAAAGTTT TATTGTA 3’ 3’UTR rhoB mut miR- 19 5’TTTGTTTTTTTATTCTTTCGA GAATTGTTTCATTGTTTGACA CTT 3’ 5’AAGTGTCAAACAATGAAACAATT CTCGAAAGAATAAAAAAACAAA 3’ 3’UTR rhoB mut-HuR5’CTGATGTTATTTGATTTAAG AAAAGGCTAAAATTTG 3’ 5’CAAATTTTAGCCTTTTCTTAAATC AAATAACATCAG 3’ 3’UTR rhoB  ’GCTCTCGAGTCTGAAGAGC CGGGCCT 3’ 5’GGACTCGAGTGCCGGCAGGGG CAGG 3’ 3’UTR rhoB  ’TGCTCTCGAGTTGACACTTA ATGCACTCGT 3’ 5’GGACTCGAGTGCCGGCAGGGG CAGG 3’ 3’UTR rhoB  ’GCTCTCGAGTAAAGGGCAG TAACAAGTATTG 3‘ 5’GGACTCGAGTGCCGGCAGGGG CAGG 3’ 3’UTR rhoB  ’GCTCTCGAGTGACAAAATG GTGAGCTTATG 3’ 5’GCTCTCGAGTGACAAAATGGTG AGCTTATG 3‘ 3’UTR rhoB  ’GCTCTCGAGTGACAAAATG GTGAGCTTATG 3’ 5’GCTCTCGAGCAGGCACAAAGTT CGCTTAT 3‘ Sequences of oligonucleotides used for mutagenesis and cloning

Table S3 siHuR5’-AAGAGGCAATTACCAGTTTCA-3’ (Kawai et al., 2006) siHuR’Pool of 5’-AATCTTAAGTTTCGTAAGTTA-3’ 5’-TTCGTAAGTTATTTCCTTTAA-3’ 5’-TTCCTTTAAGATATATATTAA-3’ (Gorospe et al., 2008) siCtrl5’-ACUCUAUCUGCACGCUGACUU-3’ Sequences of siRNAs used in this study. Kawai et al., Mol Cell Biol. (2006) 26(8): Galban et al., Mol Cell Biol. (2008) 28(1):93-107

Table S4 RhoB Forward5’-GTGCCTGCTGATCGTGTTC-3’ RhoB Reverse 5’-GCGGTCGTAGTCCTCCTG-3’ Beta-actin Forward 5’-CCTCGCCTTTGCCGATCCG-3’ Beta-actin Reverse 5’-ATGCCGGAGCCGTTGTCG-3’ p53 Forward 5’-GTGGTGGTGCCCTATGAG-3’ p53 Forward 5’-GAGTCTTCCAGTGTGATGATG-3’ GAPDH Forward 5’-TGCACCACCAACTGCTTAGC-3’ GAPDH Reverse5’-GGCATGGACTGTGGTCATGAG-3’ RLuc Forward 5’-TGGTAACGCGGCCTCTTC-3’ RLuc Reverse 5’-ATTTGCCTGATTTGCCCATAC-3’ FLuc Forward 5’-GGATGGAACCGCTGGAGAG-3’ Fluc Reverse 5’-GCTTCTGCCAACCGAACG-3’ Sequences of primers used for RTqPCR experiments