The Microscope.  Spectroscopic Microscope  Light – Compound (Bright field)  Fluorescent  Confocal  Electron- Transmission and scanning.

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Presentation transcript:

The Microscope

 Spectroscopic Microscope  Light – Compound (Bright field)  Fluorescent  Confocal  Electron- Transmission and scanning

Fluorescence Microscopy Confocal Microscopy

Illuminating system  Light source  Condenser lens- immediately below specimen  Iris diaphragm- controls amount of light

Imaging system  Objectives-4X, 10X, 40X  Ocular- lens to look through; monocular & binocular  Oculars magnify 10 times  Stage- place where specimen are mounted

Magnification  Magnification total= Mag obj X Mag ocu  Parfocal  Parcentered  Field of view

Stages of making a slide  Sectioning-blade  Mounting-(wet)- glycerin; water  Dehydration-alcohol  Fixing-formaldehyde; glutaraldehyde

Determining size of field  Micrometers  Ocular micrometer  Stage micrometer  FOV

Use of the micrometer Demonstration