The Microscope
Spectroscopic Microscope Light – Compound (Bright field) Fluorescent Confocal Electron- Transmission and scanning
Fluorescence Microscopy Confocal Microscopy
Illuminating system Light source Condenser lens- immediately below specimen Iris diaphragm- controls amount of light
Imaging system Objectives-4X, 10X, 40X Ocular- lens to look through; monocular & binocular Oculars magnify 10 times Stage- place where specimen are mounted
Magnification Magnification total= Mag obj X Mag ocu Parfocal Parcentered Field of view
Stages of making a slide Sectioning-blade Mounting-(wet)- glycerin; water Dehydration-alcohol Fixing-formaldehyde; glutaraldehyde
Determining size of field Micrometers Ocular micrometer Stage micrometer FOV
Use of the micrometer Demonstration