Laboratoire national de métrologie et d’essais Metrology to support a validated effect based monitoring strategy within the Water Framework Directive (WFD-2013/60/EC). Proof of concept for high concern pollutants Sophie LARDY-FONTAN, Béatrice LALERE, Sophie VASLIN-REIMANN

1st December 20152EMPIR Workshop on Environment call CONTEXT

1st December 20153EMPIR Workshop on Environment call SCREENING TOOLS Pressures and impact assessment EARLY WARNING SYSTEM Priorization of areas that are not supposed to be at risk MIXTURES EFFECTS OF POLLUTANTS AND UNANALYZED/UNKNOWN CHEMICALS SUPPORT WATER AND SEDIMENT QUALITY ASSESSMENT EFFECT BASED MONITORING WHAT FOR ? CONTEXT

1st December 20154EMPIR Workshop on Environment call  Further standardization of effect-based tools such as in vitro bioassays for regulatory applications and use for surface water monitoring is needed (Kase et al., 2009).  ISO,CEN works are in progress  A common validation framework that can also cover tools that are less established is therefore valuable to increase comparability of such data from different regions.  NORMAN protocol is under negotiation at CEN level (CEN TC 230 Water Analysis) Need of METROLOGICAL INFRASTRUCTURE? CONTEXT

1st December 20155EMPIR Workshop on Environment call BEQ pg eq.TCDD/g sample pg congener/g sample pg eq.TCDD/g sample A SUCCESSFULL STORY: FOOD CONTROL OF DIOXINS STOP Comparison to ML CALUX > < REFERENCE METHOD BIOANALYTICAL SCREENING Comparison to ML

1st December 20156EMPIR Workshop on Environment call The European Commission recognized the importance of assessing the endocrine disrupting potential of individual chemicals as well as, where appropriate, the cumulative impact of identified combinations of substances on the endocrine system (European Commission 2011). Watch List (Directive 2013/39/EC)  17-alpha-ethinylestradiol (EE2): EQS = 0.035ng/l  17-beta-estradiol (E2): EQS =0.4 ng/l  Estrone as degradation product Capability of conventional method questionable if not possible Effect based Tools as an alternative And now, for Water monitoring Working Group Chemicals October 2015 : Bioanalytics strategies: priority for MS and the Commission

1st December 20157EMPIR Workshop on Environment call  Definition of measurand  Traceability  Calibration  Accuracy,  Uncertainty  Definition of reference  Quality control  Harmonization of methodology  ENDOCRINE DISRUPTORS  Estrogens: EE2,E2,E1  Bioessays  Speedup TRL to reach the market  Operational Effect based Monitoring  SMART Monitoring  Standardization bodies Initial proposal for discussion Other possibilities exist : biomarkers, other type of effects, etc.