Inquiry Lab- Investigating Cheese “Microbiomes”

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Presentation transcript:

Inquiry Lab- Investigating Cheese “Microbiomes” Essential Question: How do biologists study living things?

Cheesy History 5000 BC (?) - Pouches made from animal stomachs likely provided the Rennet enzymes separating milk casein into curds and whey The practice of salting foods for preservation included curdled milk Late 1890’s - Identifying and culturing bacteria led to standardization of cheese products

Cheese Production

Ancient Biotechnology Diversity of microbes in cheese making

Cheese as a model system Culturing cheese microbes is helping scientists learn about interactions within microbial communities Blue Cheese microbes on a Petri dish

Different cheeses have different microbial communities

Resources (listed from popular to technical sources) Bacteria in Cheese The Most Delicious Lab Microbe Managing - The Life Saving Potential of Cheese Abstract: Diversity of Bacteria… in Cheese Production DNA technology reveals microbes in cheese

Experimental Design Doing things in a scientific way…. Observe Wonder Question PLAY (build a model, perform an experiment)

Observe Culture a cheese microbiome Prepare nutrient agar in Petri dishes Determine number of dishes you will need Weigh,measure, and mix powder in a flask Stir and boil until solution turns translucent Cool, then pour thin layers into plates Bring in cheese samples (washed rind) Using sterile swabs innoculate agar from cheese rind samples After several days, LOOK at growth!

Wonder What kind of bacteria are growing? Is the community stable or dynamic? How could we change the community?

Question What condition causes a particular bacterial species to increase in number? Decrease in diversity? Die off completely?

Experimental Design Guidelines (IF) Independent Variable: condition or situation you will set up (THEN) Dependent Variable: Must be quantitative the NUMBER that you will record

IF/THEN hypothesis should reflect your question Hypothesis predicts how variables will change IF the temperature increases, THEN population of Bac A will increase.

Data collection should reflect your question Independent Variable (x-axis) = Dependent Variable (y-axis) =