Supplementary Material 3 Gene ontology annotation of cellular component, molecular function and biological processes for both hypoxia and NAP supplemented.

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Supplementary Material 3 Gene ontology annotation of cellular component, molecular function and biological processes for both hypoxia and NAP supplemented hypoxia after 3, 6 and 12 h of hypobaric hypoxia exposure. All the identified pathways were sorted according to the significance value.

3h hypoxia Cellular Component IDNameP-value Hit Count in Query List Hit Count in GenomeHit in Query List GO: mitochondrion CRYM,AK1,GAPDH,SOD1,COX6A1,C OX5A,ATP5C1,NDUFA10,GPD1,COX5 B,PPP3CA,DPYSL2,MCL1,ALKBH3,MP ST,PSMA6 GO: mitochondrial membrane COX6A1,COX5A,ATP5C1,NDUFA10,C OX5B,MCL1,MPST GO: mitochondrial inner membrane COX6A1,COX5A,ATP5C1,NDUFA10,C OX5B,MPST GO: proteasome core complex, alpha-subunit complex PSMA6,PSMA1 GO: hemoglobin complex HBB,HBA1 3h hypoxia + NAP Cellular Component IDNameP-value Hit Count in Query List Hit Count in GenomeHit in Query List GO: mitochondrion CRYM,AK1,PSMC2,GAPDH,SOD1,PRD X2,COX5A,COX6A1,ATP5C1,NDUFA1 0,GPD1,COX5B,PPP3CA,DPYSL2,MPS T GO: soluble fraction ENO1,PAFAH1B1,GPD1,PPP3CA,DPYS L2,HBA1,TPI1,ACTB GO: neuron projection AK1,PTK2B,SYT1,CA2,SOD1,PAFAH1 B1,DPYSL2,ACTB GO: cell projection AK1,PTK2B,SYT1,CA2,SOD1,CDC42,P AFAH1B1,GNB1,DPYSL2,ACTB GO: mitochondrial inner membrane COX5A,COX6A1,ATP5C1,NDUFA10,C OX5B,MPST GO: cell fraction VCP,ENO1,GDI1,PAFAH1B1,GPD1,PPP 3CA,DPYSL2,GNAO1,HBA1,TPI1,ACT B GO: synaptosome ENO1,GDI1,DPYSL2,GNAO1 Table 1. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component.

Figure 1. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component (3H hypoxia and 3HT-NAP supplemented hypoxia).

3h hypoxia Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Oxidoreductase activity GAPDH,SOD1,COX6A1,COX5A,NDUF A10,GPD1,COX5B,AKR1B1,ALKBH3 GO: Cytochrome-c oxidase activity COX6A1,COX5A,COX5B GO: Oxidoreductase activity, acting on a heme group of donors, oxygen as acceptor COX6A1,COX5A,COX5B GO: Hydrogen ion transmembrane transporter activity COX6A1,COX5A,ATP5C1,COX5B 3h hypoxia +NAP Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Cytochrome-c oxidase activity COX5A,COX6A1,COX5B GO: Oxidoreductase activity, acting on a heme group of donors COX5A,COX6A1,COX5B GO: Identical protein binding VCP,CRYM,SYT1,ENO1,SOD1,PAFAH 1B1,GPD1,KITLG,DNM1 GO: Hydrogen ion transmembrane transporter activity COX5A,COX6A1,ATP5C1,COX5B Table 2. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function.

Figure 2. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function (3H hypoxia and 3HT-NAP supplemented hypoxia).

3h hypoxia Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Generation of precursor metabolites and energy GAPDH,COX6A1,COX5A,ATP5C1,ND UFA10,GNB1,COX5B GO: Oxidation-reduction process GAPDH,SOD1,COX6A1,COX5A,ATP5 C1,NDUFA10,GPD1,GNB1,COX5B,AK R1B1,ALKBH3,CAB39 GO: Respiratory electron transport chain COX6A1,COX5A,ATP5C1,NDUFA10, COX5B GO: Response to hydrogen peroxide HBB,PTK2B,SOD1,GNAO1 GO: Response to reactive oxygen species HBB,PTK2B,SOD1,GNAO1 GO: Energy derivation by oxidation of organic compounds COX6A1,COX5A,ATP5C1,NDUFA10, GNB1,COX5B GO: Response to calcium ion PTK2B,SYT1,GDI1,ACTB 3h hypoxia +NAP Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Generation of precursor metabolites and energy GAPDH,ENO1,COX5A,COX6A1,ATP5 C1,NDUFA10,GNB1,COX5B,TPI1 GO: Oxidation-reduction process GAPDH,SOD1,PRDX2,COX5A,COX6 A1,ATP5C1,NDUFA10,GPD1,GNB1,C OX5B,TPI1,CAB39 GO: Respiratory electron transport chain COX5A,COX6A1,ATP5C1,NDUFA10, COX5B GO: Response to hydrogen peroxide HBB,PTK2B,SOD1,PRDX2,GNAO1 GO: Response to reactive oxygen species HBB,PTK2B,SOD1,PRDX2,GNAO1 GO: Response to calcium ion PTK2B,SYT1,GDI1,ACTB GO: Nucleotide metabolic process VCP,AK1,PSMC2,PTK2B,GDI1,ATP5C 1,PAFAH1B1,GPD1,GNAO1,TPI1,CMP K1,DNM1 GO: Carboxylic acid metabolic process PSMC5,CRYM,PSMC2,GAPDH,ENO1, SOD1,GPD1,GSTP1,TPI1,CAB39 GO: Positive regulation of molecular function PSMC5,VCP,PSMC2,PTK2B,ENO1,SO D1,PRDX2,GNB1,GNAO1,KITLG Table 3. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process.

Figure 3. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process (3H hypoxia and 3HT-NAP supplemented hypoxia).

6h hypoxia Cellular Component IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Mitochondrion AK1,PSMC2,GAPDH,SOD1,COX5A,COX6A1,ATP 5C1,PRDX5,NDUFA10,DDAH1,GPD1,COX5B,DP YSL2,MCL1,UQCRFS1,PEBP1,ALKBH3,MPST,GL UL GO: Mitochondrial membrane COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,MCL 1,UQCRFS1,PEBP1,MPST GO: Axon PTK2B,CA2,DPYSL2,PEBP1,GLUL,ACTB GO: Soluble fraction GPD1,RAB1A,DPYSL2,PEBP1,HBA1,GLUL,ACTB GO: Synaptosome GDI1,DPYSL2,PEBP1,GLUL GO: Proteasome complex VCP,PSMC2,PSMA1 GO: Mitochondrial respiratory chain COX6A1,NDUFA10,UQCRFS1 GO: Neuron projection AK1,PTK2B,CA2,SOD1,DPYSL2,PEBP1,GLUL,AC TB GO: Mitochondrial part SOD1,COX5A,COX6A1,ATP5C1,NDUFA10,COX5 B,MCL1,UQCRFS1,PEBP1,MPST GO: Organelle envelope COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,MCL 1,UQCRFS1,PEBP1,MPST GO: Cell projection AK1,PTK2B,CA2,SOD1,CDC42,DPYSL2,PEBP1,G LUL,ACTB GO: Hemoglobin complex HBB,HBA1 6h hypoxia + NAP Cellular Component IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Mitochondrion PSMC2,GAPDH,RHOA,COX5A,ACADL,UQCRFS1,PEBP1,ALKBH3,MPST,CRYM,AK1,NME1,SOD1, ACOT7,PRDX2,COX6A1,ATP5C1,DDAH1,COX5B,PPP3CA,DPYSL2,MCL1,PSMA6,GLUL,MDH1 GO: Soluble fraction ENO1,RHOA,PAFAH1B1,PEBP1,ACTB,NME1,PPP 3CA,DPYSL2,HBA1,GLUL,MDH1 GO: Proteasome complex PSMC5,VCP,PSMC2,PSMA6,PSMA1 GO: Synaptosome ENO1,PEBP1,GDI1,DPYSL2,GNAO1,GLUL GO: Mitochondrial membrane COX5A,ACADL,UQCRFS1,PEBP1,MPST,COX6A1,ATP5C1,COX5B,MCL1 GO: Mitochondrial part COX5A,ACADL,UQCRFS1,PEBP1,MPST,SOD1,C OX6A1,ATP5C1,COX5B,MCL1 GO: Cell fraction VCP,ENO1,RHOA,PAFAH1B1,PEBP1,ACTB,NME 1,GDI1,PPP3CA,DPYSL2,GNAO1,HBA1,GLUL,M DH1 GO: Organelle envelope COX5A,ACADL,PAFAH1B1,UQCRFS1,PEBP1,MP ST,COX6A1,ATP5C1,COX5B,MCL1 GO: Axon RHOA,PAFAH1B1,PEBP1,ACTB,DPYSL2,GLUL GO: Membrane fraction VCP,ENO1,RHOA,PAFAH1B1,PEBP1,NME1,GDI1,PPP3CA,DPYSL2,GNAO1,GLUL GO: Neuron projection RHOA,PAFAH1B1,PEBP1,ACTB,AK1,SOD1,DPYS L2,GLUL Table 4. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component.

Figure 4. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component (6H hypoxia and 6HT-NAP supplemented hypoxia).

6h hypoxia Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Oxidoreductase activity GAPDH,SOD1,COX5A,COX6A1,PRD X5,NDUFA10,GPD1,COX5B,UQCRF S1,ALKBH3 GO: Hydrogen ion transmembrane transporter activity COX5A,COX6A1,ATP5C1,COX5B,U QCRFS1 GO: Cytochrome-c oxidase activity COX5A,COX6A1,COX5B GO: Oxidoreductase activity, acting on a heme group of donors, oxygen as acceptor COX5A,COX6A1,COX5B GO: Inorganic cation transmembrane transporter activity COX5A,COX6A1,ATP5C1,COX5B,U QCRFS1 6h hypoxia +NAP Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Oxidoreductase activity GAPDH,COX5A,ACADL,UQCRFS1, ALKBH3,SOD1,PRDX2,COX6A1,GS TO1,COX5B,AKR1B1,MDH1 GO: Hydrogen ion transmembrane transporter activity COX5A,UQCRFS1,COX6A1,ATP5C1, COX5B GO: Carboxylic acid binding ACADL,GSTP1,ALKBH3,ACOT7,DD AH1,GLUL GO: Glutathione transferase activity GSTP1,GSTM5,GSTO1 GO: Cytochrome-c oxidase activity COX5A,COX6A1,COX5B GO: Oxidoreductase activity, acting on a heme group of donors, oxygen as acceptor COX5A,COX6A1,COX5B Table 5. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function.

Figure 5. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function (6H hypoxia and 6HT-NAP supplemented hypoxia).

6h hypoxia Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Respiratory electron transport chain COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,UQCRFS1 GO: Oxidation-reduction process GAPDH,SOD1,COX5A,COX6A1,ATP5C1,PR DX5,NDUFA10,GPD1,COX5B,UQCRFS1,AL KBH3 GO: Generation of precursor metabolites and energy GAPDH,COX5A,COX6A1,ATP5C1,NDUFA1 0,COX5B,UQCRFS1 GO: Carboxylic acid metabolic process PSMC2,GAPDH,SOD1,DDAH1,GPD1,GSTP1, PCMT1,GLUL,PSMA1 GO: Cellular respiration COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,UQCRFS1 GO: Response to calcium ion PTK2B,GDI1,PEBP1,ACTB GO: Energy derivation by oxidation of organic compounds COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,UQCRFS1 GO: Protein nitrosylation GAPDH,DDAH1 6h hypoxia +NAP Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Carboxylic acid metabolic process PSMC5,PGAM1,PSMC2,GAPDH,ENO1,ACA DL,GSTP1,CAB39,CRYM,SOD1,ACOT7,GST O1,DDAH1,PSMA6,GLUL,MDH1,PSMA1 GO: Cellular respiration COX5A,UQCRFS1,COX6A1,ATP5C1,COX5B,MDH1 GO: Cellular response to stress PSMC5,VCP,RPS6KA1,PSMC2,RHOA,ALKB H3,HBB,UBE2N,SOD1,PRDX2,AKR1B1,PSM A6,PSMA1 GO: Generation of precursor metabolites and energy PGAM1,GAPDH,ENO1,COX5A,UQCRFS1,C OX6A1,ATP5C1,GNB1,COX5B,MDH1 GO: Negative regulation of protein modification process PSMC5,PSMC2,PEBP1,PSMA6,PSMA1,IMPA CT GO: Oxidation-reduction process PGAM1,GAPDH,COX5A,ACADL,UQCRFS1, ALKBH3,CAB39,SOD1,PRDX2,COX6A1,AT P5C1,GNB1,COX5B,AKR1B1,MDH1 GO: Positive regulation of molecular function PSMC5,VCP,PSMC2,ENO1,RHOA,UBE2N,N ME1,SOD1,PRDX2,GNB1,RGN,GNAO1,PSM A6,PSMA1 GO: Respiratory electron transport chain COX5A,UQCRFS1,COX6A1,ATP5C1,COX5B GO: Positive regulation of catalytic activity PSMC5,VCP,PSMC2,RHOA,UBE2N,SOD1,P RDX2,GNB1,RGN,GNAO1,PSMA6,PSMA1 GO: Positive regulation of cell cycle process PSMC5,PSMC2,PEBP1,PSMA6,PSMA1 GO: Positive regulation of protein ubiquitination PSMC5,PSMC2,UBE2N,PSMA6,PSMA1 GO: Gluconeogenesis PGAM1,GAPDH,ENO1,MDH1 GO: Nucleobase, nucleoside and nucleotide metabolic process VCP,PGAM1,PSMC2,PAFAH1B1,DNM1,AK 1,NME1,GDI1,ATP5C1,RGN,GNAO1,MDH1 Table 6. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process.

Figure 6. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process (6H hypoxia and 6HT-NAP supplemented hypoxia).

12h hypoxia Cellular Component IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Mitochondrion PSMC2,GAPDH,RHOA,COX5A,ACADL,GP D1,PARK7,PEBP1,CRYM,AK1,NME1,SOD1, PRDX2,COX6A1,ATP5C1,PRDX5,NDUFA10, DDAH1,COX5B,PPP3CA,MCL1,GLUL GO: Soluble fraction ENO1,RHOA,PAFAH1B1,HSPB1,GPD1,RAB 1A,PEBP1,ACTB,NME1,PPP3CA,HBA1,TPI1, GLUL GO: Neuron projection PTK2B,RHOA,PAFAH1B1,PARK7,PEBP1,A CTB,AK1,SYT1,CA2,SOD1,GLUL GO: Proteasome complex PSMC5,VCP,PSMC2,HSPB1,PSMA1 GO: Axon PTK2B,RHOA,PAFAH1B1,PARK7,PEBP1,A CTB,CA2,GLUL GO: Cell projection PTK2B,RHOA,PAFAH1B1,PARK7,PEBP1,A CTB,AK1,SYT1,CA2,SOD1,CDC42,GNB1,GL UL GO: Cell fraction VCP,ENO1,RHOA,PAFAH1B1,HSPB1,GPD1, RAB1A,PEBP1,ACTB,NME1,PPP3CA,GNAO 1,HBA1,TPI1,GLUL GO: Mitochondrial membrane COX5A,ACADL,PEBP1,COX6A1,ATP5C1,N DUFA10,COX5B,MCL1 12h hypoxia +NAP Cellular Component IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Mitochondrion AK1,NME1,RHOA,SOD1,PRDX2,COX6A1,C OX5A,ATP5C1,PRDX5,NDUFA10,DDAH1,G PD1,PPP3CA,PARK7,MCL1,PEBP1 GO: Soluble fraction ENO1,NME1,RHOA,GPD1,RAB1A,PPP3CA, PEBP1,HBA1,TPI1 GO: Neuron projection AK1,PTK2B,SYT1,CA2,RHOA,SOD1,PARK7,PEBP1 GO: Cell fraction VCP,ENO1,NME1,RHOA,GPD1,RAB1A,PPP 3CA,GNAO1,PEBP1,HBA1,TPI1 GO: Axon PTK2B,CA2,RHOA,PARK7,PEBP1 GO: Hemoglobin complex HBB,HBA1 GO: Mitochondrial membrane COX6A1,COX5A,ATP5C1,NDUFA10,MCL1, PEBP1 Table 7. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component.

Figure 7. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component (12H hypoxia and 12HT-NAP supplemented hypoxia).

12h hypoxia Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Identical protein binding VCP,ENO1,PAFAH1B1,HSPB1,GPD1,KITLG,DNM1,CRYM,SYT1,NME1,SOD1,GSTA4,P CMT1,GLUL GO: Peroxiredoxin activity PARK7,PRDX2,PRDX5 GO: Oxidoreductase activity GAPDH,COX5A,ACADL,GPD1,PARK7,SOD 1,PRDX2,COX6A1,PRDX5,NDUFA10,GSTO 1,COX5B GO: GTPase activity RHOA,RAB1A,DNM1,CDC42,ARF1,GNB1,G NAO1 GO: Nucleoside-triphosphatase activity PSMC5,VCP,PSMC2,RHOA,RAB1A,DNM1,C DC42,ATP5C1,ARF1,GNB1,GNAO1 GO: GTP binding RHOA,RAB19,RAB1A,DNM1,NME1,CDC42, ARF1,GNAO1 GO: Guanyl nucleotide binding RHOA,RAB19,RAB1A,DNM1,NME1,CDC42, ARF1,GNAO1 GO: Hydrolase activity, acting on acid anhydrides, in phosphorus- containing anhydrides PSMC5,VCP,PSMC2,RHOA,RAB1A,DNM1,C DC42,ATP5C1,ARF1,GNB1,GNAO1 GO: Glutathione transferase activity GSTM5,GSTA4,GSTO1 GO: Cytochrome-c oxidase activity COX5A,COX6A1,COX5B 12h hypoxia + NAP Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Peroxiredoxin activity PRDX2,PRDX5,PARK7 GO: Phosphotransferase activity, phosphate group as acceptor AK1,NME1,CMPK1 GO: GTPase activity RHOA,ARF1,RAB1A,GNAO1,DNM1 GO: Oxidoreductase activity SOD1,PRDX2,COX6A1,COX5A,PRDX5,NDU FA10,GPD1,PARK7 GO: GTP binding NME1,RHOA,ARF1,RAB1A,GNAO1,DNM1 GO: Guanyl nucleotide binding NME1,RHOA,ARF1,RAB1A,GNAO1,DNM1 GO: Antioxidant activity SOD1,PRDX2,PRDX5 Table 8. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function.

Figure 8. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function (12H hypoxia and 12HT-NAP supplemented hypoxia).

12h hypoxia Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Response to reactive oxygen species PTK2B,PARK7,HBB,SOD1,PRDX2,PRDX5,G NAO1 GO: Response to hydrogen peroxide PTK2B,PARK7,HBB,SOD1,PRDX2,GNAO1 GO: Response to oxidative stress PTK2B,PARK7,PEBP1,HBB,SOD1,PRDX2,PR DX5,GNAO1 GO: Nucleotide metabolic process VCP,PGAM1,PSMC2,PTK2B,PAFAH1B1,GP D1,CMPK1,DNM1,AK1,NME1,ATP5C1,GNA O1,TPI1 GO: Oxidation-reduction process PGAM1,GAPDH,COX5A,ACADL,GPD1,CAB 39,SOD1,PRDX2,COX6A1,ATP5C1,PRDX5,N DUFA10,GNB1,COX5B,TPI1 GO: Positive regulation of molecular function PSMC5,VCP,PSMC2,PTK2B,ENO1,RHOA,KI TLG,NME1,SOD1,PRDX2,GNB1,GNAO1,PS MA1 GO: Oxygen and reactive oxygen species metabolic process PTK2B,PARK7,HBB,SOD1,PRDX2 GO: Cellular response to reactive oxygen species HBB,SOD1,PRDX2,PRDX5 GO: Cellular homeostasis PTK2B,PARK7,CA2,SOD1,PRDX2,PRDX5,A RF1,GNB1,PPP3CA,MCL1 GO: Nucleotide phosphorylation CMPK1,AK1,NME1 GO: Carboxylic acid metabolic process PSMC5,PGAM1,PSMC2,GAPDH,ENO1,ACA DL,GPD1,CAB39,CRYM,SOD1,GSTA4,GSTO 1,DDAH1,PCMT1,TPI1,GLUL,PSMA1 GO: Gluconeogenesis PGAM1,GAPDH,ENO1,GPD1,TPI1 GO: Generation of precursor metabolites and energy PGAM1,GAPDH,ENO1,COX5A,COX6A1,AT P5C1,NDUFA10,GNB1,COX5B,TPI1 GO: Regulation of cell death PSMC5,VCP,PSMC2,PTK2B,RHOA,HSPB1,K ITLG,HBB,NME1,SOD1,PRDX2,PRDX5,MCL 1,PSMA1 GO: Hydrogen peroxide metabolic process PARK7,HBB,SOD1,PRDX2 GO: Protein complex assembly VCP,PTK2B,RHOA,ACADL,CAB39,HBB,SY T1,CDC42,HBA1,GLUL GO: Respiratory electron transport chain COX5A,COX6A1,ATP5C1,NDUFA10,COX5B GO: Positive regulation of catalytic activity PSMC5,VCP,PSMC2,PTK2B,RHOA,KITLG,S OD1,PRDX2,GNB1,GNAO1,PSMA1 GO: Protein oligomerization VCP,ACADL,CAB39,HBB,SYT1,HBA1,GLUL GO: Homeostatic process PTK2B,ACADL,PARK7,KITLG,CA2,SOD1,P RDX2,PRDX5,ARF1,GNB1,PPP3CA,MCL1,H BA1 Table 9a. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed hippocampus. The distribution of identified proteins according to their biological process.

12h hypoxia +NAP Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO: Response to hydrogen peroxide HBB,PTK2B,SOD1,PRDX2,PARK7,GNAO1 GO: Response to oxidative stress HBB,PTK2B,SOD1,PRDX2,PRDX5,PARK7, GNAO1,PEBP1 GO: Response to reactive oxygen species HBB,PTK2B,SOD1,PRDX2,PRDX5,PARK7, GNAO1 GO: Nucleotide metabolic process VCP,AK1,PTK2B,NME1,ATP5C1,GPD1,GN AO1,TPI1,CMPK1,DNM1 GO: Oxidation-reduction process SOD1,PRDX2,COX6A1,COX5A,ATP5C1,PR DX5,NDUFA10,GPD1,TPI1,CAB39 GO: Positive regulation of molecular function VCP,PTK2B,UBE2N,ENO1,NME1,RHOA,SO D1,PRDX2,GNAO1 GO: Cellular response to reactive oxygen species HBB,SOD1,PRDX2,PRDX5 GO: Oxygen and reactive oxygen species metabolic process HBB,PTK2B,SOD1,PRDX2,PARK7 GO: Cellular homeostasis PTK2B,CA2,SOD1,PRDX2,PRDX5,ARF1,PP P3CA,PARK7,MCL1 GO: Nucleotide phosphorylation AK1,NME1,CMPK1 GO: Cellular response to oxidative stress HBB,SOD1,PRDX2,PRDX5 GO: Circulatory system process HBB,RHOA,SOD1,GNAO1,PEBP1,HBA1 Table 9b. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia with NAP supplemented hippocampus. The distribution of identified proteins according to their biological process.

Figure 9. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process (12H hypoxia and 12HT-NAP supplemented hypoxia).