DNA Nanorobotics Reem Mokhtar. DNA Nanorobotics Book Chapter: Chandran, H., Gopalkrishnan, N., & Reif, J. (n.d.). DNA Nanorobotics. Aim: design and fabrication.

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DNA Nanorobotics Reem Mokhtar

DNA Nanorobotics Book Chapter: Chandran, H., Gopalkrishnan, N., & Reif, J. (n.d.). DNA Nanorobotics. Aim: design and fabrication of dynamic DNA nanostructures that perform specific tasks via a series of state changes (average system behavior)

Challenges 1.Domain to sequence mapping in design (spurious interactions) 2.Fuel (spontaneous entropic effects) 3.Assembly + purification 4.Experimental design for validation...each step is a challenge Book Chapter: Chandran, H., Gopalkrishnan, N., & Reif, J. (n.d.). DNA Nanorobotics.

Examples in Nature –> Kinesins ATPases (ATP  ADP  energy) Book Chapter: Chandran, H., Gopalkrishnan, N., & Reif, J. (n.d.). DNA Nanorobotics. Yildiz, A. (2004). Kinesin Walks Hand-Over-Hand. Science, 303(5658), 676–678. doi: /science

Examples in Nature –> Myosins Protein Toyoshima, Y. Y., Kron, S. J., McNally, E. M., Niebling, K. R., Toyoshima, C., & Spudich, J. A. (1987). Myosin subfragment-1 is sufficient to move actin filaments in vitro. Binds to actin  ATP hydrolysis  mechanical movement Book Chapter: Chandran, H., Gopalkrishnan, N., & Reif, J. (n.d.). DNA Nanorobotics.

Why DNA? Design 1.Predictable geometries can be achieved 2.Can predict dsDNA thermodynamics + geometry using software 3.Software-assisted design Book Chapter: Chandran, H., Gopalkrishnan, N., & Reif, J. (n.d.). DNA Nanorobotics.

Why DNA? Experiments 1.Custom ssDNA synthesis  routine + inexpensive 2.Assembly simple 3.Characterization techniques (AFM, TEM, …) Book Chapter: Chandran, H., Gopalkrishnan, N., & Reif, J. (n.d.). DNA Nanorobotics.

Chapter Classification Switch conformation based on environment Powered by enzymatic reactions Hybridization reactionsProgrammable Book Chapter: Chandran, H., Gopalkrishnan, N., & Reif, J. (n.d.). DNA Nanorobotics.