Microscopy Large Features ( »  ) Real Space: x Single Object

Slides:

Advertisements

Similar presentations

X-ray Diffraction. X-ray Generation X-ray tube (sealed) Pure metal target (Cu) Electrons remover inner-shell electrons from target. Other electrons “fall”

Advertisements

Phys 102 – Lecture 23 Diffraction.

Determination of Protein Structure. Methods for Determining Structures X-ray crystallography – uses an X-ray diffraction pattern and electron density.

4. Investigations into the electrical properties of particular metals at different temperatures led to the identification of superconductivity and the.

End Show Slide 1 of 21 Copyright Pearson Prentice Hall 12-2 Chromosomes and DNA Replication 12–2 The Structure of DNA.

(0,0) RECIPROCAL LATTICE (0,1) (1,1) (2,1) (3,1) REAL LATTICE a b a* b*

Chapter 11 1 Ch 11 Page 467 Intermolecular forces according to Google Images:

Crystallography and Diffraction Techniques Myoglobin.

Diffraction See Chapter 10 of Hecht.

Solid State Physics 2. X-ray Diffraction 4/15/2017.

1 Experimental Determination of Crystal Structure Introduction to Solid State Physics

Nanostructured functional films from engineered repeat proteins by Tijana Z. Grove, Lynne Regan, and Aitziber L. Cortajarena Interface Volume 10(83):

Nuclear Instability.

Chem 125 Lecture 5 9/12/08 Projected material This material is for the exclusive use of Chem 125 students at Yale and may not be copied or distributed.

Imaging with Waves. Creating images with light camera obscuramodern imaging device.

Scattering Experiments

Chem 125 Lecture 7 9/20/06 Projected material This material is for the exclusive use of Chem 125 students at Yale and may not be copied or distributed.

PHY 102: Waves & Quanta Topic 8 Diffraction II John Cockburn Room E15)

Physics 1C Lecture 27C Quiz # on Monday: All of Chapters 26 and 27.

Happyphysics.com Physics Lecture Resources Prof. Mineesh Gulati Head-Physics Wing Happy Model Hr. Sec. School, Udhampur, J&K Website: happyphysics.com.

The Structure of DNA Pgs For General Biology (as well as building on your prior knowledge of organic molecules, pg 1 st semester.

Section 8.2: The Structure of DNA

CHE (Structural Inorganic Chemistry) X-ray Diffraction & Crystallography lecture 2 Dr Rob Jackson LJ1.16,

Hon. Biology Period 6. Nucleic Acids Nucleic acids are large complex organic molecules composed of carbon, oxygen, hydrogen, nitrogen, and phosphorus.

Chapter 7 X-Ray diffraction. Contents Basic concepts and definitions Basic concepts and definitions Waves and X-rays Waves and X-rays Crystal structure.

X. Low energy electron diffraction (LEED)

Rosalind Franklin and X-ray Diffraction. Rosalind Franklin Born in July 25, 1920 in London, England Died April 16,1958 in London, England (ovarian cancer)

A Brief History. Rosalind Franklin Born: July 1920 Notting Hill, London Fields X-ray crystallography Alma Mater: Newham College, Cambridge Died: April.

Chp 4.2- Structure of the Atom

Length Scales in Physics, Chemistry, Biology,… Length scales are useful to get a quick idea what will happen when making objects smaller and smaller. For.

Lesson Overview 12.2 The Structure of DNA. The Components of DNA What are the chemical components of DNA? DNA is a nucleic acid made up of nucleotides.

1 Review List the chemical components of DNA Relate Cause and Effect Why are hydrogen bonds so essential to the structure of DNA 2 Review Describe the.

Analysing Nanostructures

X-Ray Diffraction Dr. T. Ramlochan March 2010.

The units of Planck's constant h are joule-s, and the frequency of a light wave is f. The expression for the energy of a photon is (a) h / f(b) hf(c) f.

2   y k y  rows ky =ky = Diffraction kyky yy Reciprocal space k x, k y Real space x, y.

Why Diffraction, Why Neutrons? J. A. Dura Neutron Small Angle Scattering and Reflectometry NCNR Summer School on June 26, 2006.

1. Diffraction intensity 2. Patterson map Lecture

Chapter 01. A Singular Theme Basic structures and mechanisms that sustain life are common to all living creatures All forms of life are connected to one.

STRUCTURAL BIOLOGY Martina Mijušković ETH Zürich, Switzerland.

Light of wavelength passes through a single slit of width a. The diffraction pattern is observed on a screen a distance x from the slit. Q double.

 Rosalind Franklin was asked to set up a DNA laboratory using X-ray technology.  Rosalind Franklin was best well known for her X-ray diffraction images.

Center for Materials for Information Technology an NSF Materials Science and Engineering Center Scattering Techniques Lecture 17 G.J. Mankey

Chapter 7 Lecture Lecture Presentation Chapter 7 The Quantum- Mechanical Model of the Atom Sherril Soman Grand Valley State University © 2014 Pearson Education,

Announcements Grades (for Mid-term) in Web portal

X-ray diffraction X-rays discovered in 1895 – 1 week later first image of hand. X-rays have ~ 0.1 – few A No lenses yet developed for x-rays – so no possibility.

X-Ray Diffraction By Cade Grigsby.

Structure of proteins by X-ray crystallography

DNA (Deoxyribonucleic Acid)

The Structure and Dynamics of Solids

X-Ray Diffraction Spring 2011.

Information molecules

Lecture 10 CS566 Fall Structural Bioinformatics Motivation Concepts Structure Solving Structure Comparison Structure Prediction Modeling Structural.

Lecture_08: Outline Matter Waves  de Broglie hypothesis  Experimental verifications  Wave functions.

Crystallography : How do you do? From Diffraction to structure…. Normally one would use a microscope to view very small objects. If we use a light microscope.

Crystal Structure and Crystallography of Materials Chapter 13: Diffraction Lecture No. 1.

Lecture 53: X-ray crystallography. Electrons deflect x-rays We try to recreate electron density from the x-ray diffraction pattern Each point in space.

Cont. Proteomics Structural Genomics Describes the experimental and analytical techniques that are used to determine the structure of proteins and RNA.

Q1.1 Find the wavelength of light used in this 2- slits interference.

3.3 Other types of microscopy

Reciprocal Lattice the the.

Light waves White light is a mixture of different colors.

de Broglie Waves de Broglie argued

Introduction to Bioinformatics II

Diffraction Interference of waves creates a diffraction pattern.

Structure of the Atom (Section 4.2)

Chapter 16: Electron Diffraction

Presentation transcript:

Microscopy Large Features ( »  ) Real Space: x Single Object Diffraction Small Features (   ) Reciprocal Space: kx = 2/x Ordered Array versus

Real space versus reciprocal space kx = 2/x x = row spacing kx  rows ky y Real space: x,y,z Reciprocal space: kx, ky, kz

Test patterns for simulating diffraction from DNA Single helix Double helix

Rosalind Franklin’s x-ray diffraction pattern of DNA, which led to the double-helix model (Linus Pauling’s copy)

X-ray diffraction pattern of DNA Diffraction pattern The double helix of DNA  2 b 2 p p b  p = period of one turn b = base pair spacing  = slope of the helix

X-ray diffraction image of the protein myoglobin This image contains about 3000 diffraction spots. All that information is needed to determine the positions of thousands of atoms in myoglobin. Protein crystallography has become essential for biochemistry, because the structure of a protein determines its function .

Real space versus reciprocal space Diffraction patterns live in reciprocal space, which corresponds to the projection screen. A direction of a beam in real space becomes a point on the screen in reciprocal space. Everything is backwards in reciprocal space: A large distance x in real space becomes a small k-vector kx in reciprocal space and vice versa. Even physicists have a hard time thinking in reciprocal space. But it is used widely for characterizing waves, particularly electron waves in solids and nanostructures.

Low Energy Electron Diffraction (LEED) at surfaces K = 2/d k = 2/D D d 1D chain structure 2D planar structure

Neutron Diffraction: Small Angle Neutron Scattering (SANS) Good for light elements (hydrogen, deuterium, polymers) and for magnetic materials (magnetic moment of the neutron). Q1/2  1/Rg P Q Rg a Model of a polymer: Rg = radius of gyration a  persistence length (see Lecture 2 on length scales) Diffracted neutron intensity P plotted versus the k-vector Q