Composite Silica:Polypeptide Nanoparticles

Slides:



Advertisements
Similar presentations
Module A-2: SYNTHESIS & ASSEMBLY
Advertisements

Strategies to build mixed DNA and PEG films on silica surfaces to achieve molecularly uniform biosensing conditions Acknowledgements Conclusion It is evident.
Polymer Synthesis CHEM 421 Polycarbonates: Interfacial Polymerizations Commercially Important Commercially Important Brunelle, D. J. Am. Chem. Soc., 1990,
The study of cysteine molecule coated magnetic Fe 3 O 4 nanoparticles via sonochemical method for bio-applications Kevin J. Schilling, Joo Seob Lee, and.
Nanoparticles Characterization: Measurement of the particles size by the PCS technique MSc. Priscyla D. Marcato Dr. Nelson Durán.
Nanoscience at UMCP. Department of Chemistry and Biochemistry Faculty: Jeff Davis, Bryan Eichhorn, Doug English, Lyle Isaacs, Jason Kahn, Janice Reutt-Robey,
Chemical Vapor Deposition ( CVD). Chemical vapour deposition (CVD) synthesis is achieved by putting a carbon source in the gas phase and using an energy.
Thin Film Deposition Prof. Dr. Ir. Djoko Hartanto MSc
7. Paint Industrial Products (Testing). basic function of a paint protecting a surface from the action of light, water, and air achieved by the application.
 Molar Mass And Molar Mass Distribution Molecular Weight Determination Laser Light Scattering Chromatography Size Exclusion (GPC) Mass Spectroscopy.
Unit 2, Part 3: Characterizing Nanostructure Size Dr. Brian Grady-Lecturer
Magnetic Core/Shell Nanocomposites Mohamed Darwish Institute of Nanomaterials, Advanced Technology and Innovation Technical University of Liberec 23/4/2013.
Schafer Corporation An Employee-Owned Small Business Schafer Laboratories Schafer Corporation at Sandia National Laboratory 1515 Eubank SE, M.S 1196 Albuquerque,
Separation of Mixtures
Side Chain Liquid Crystalline Polymers Polymers with mesogens attached as side chains can exhibit liquid crystalline properties. The extent to which.
Science and Technology of Nano Materials
Surface Modification for Biomaterials Applications
Triglyceride crystallization model systems for polymer crystallization? melt Poly(propylene-co-1-pentene) for a better impact/stiffness.
Non-covalent modification of luminescent Tb-TCAS-doped silica nanoparticles surface by surfactants. Bochkova O.D., Fedorenko S.V. Elistratova Yu.G., Mustafina.
Surface Engineering on Optically Transparent Materials: Extreme Surface Wetting, Anti-Fogging Behavior, and Enhanced Optical Transmittance Robert A. Fleming.
Figure 5: (a) Confocal section of m-PEG particle distribution (green) in a tumor spheroid (nuclei stained blue) (40x magnification) (b) Confocal section.
Synthesis Rutile titania nanofibers are synthesized using electrospinning and sol-gel coating techniques. A large sheet of nylon-6 nanofibers are synthesized.
Lomonosov Moscow State University Physics Department SPECIFIC INTERACTION OF ALBUMIN MOLECULES IN WATER SOLUTION, CONTAINING SILICON NANOPARTICLES AT DIFFERENT.
Matter - Properties and Changes Chapter 3. Substances Substance = Matter that has a uniform and unchanging composition Examples are salt and water Is.
Optics on a Nanoscale Using Polaritonic and Plasmonic Materials (NSF NIRT ) Andrey Chabanov 1, Federico Capasso 2, Vinothan Manoharan 2, Michael.
NIRT: Magnetically and Thermally Active Nanoparticles for Cancer Treatment (CBET ) Carlos Rinaldi, Madeline Torres-Lugo, Gustavo Gutierrez, J. Zach.
Chapter 2: How Atoms Are Combined J.F. Thompson, Ph.D.
FIG. 5.1 Multiple scattering is viewed as a random walk of the photon in diffusing wave spectroscopy (DWS)
High Anisotropy Magnetic Nanoparticles and Nanocomposites G.C Hadjipanayis, University of Delaware Tel: (302) NSF MET DMR
Unit 2. Unit 2 - Matter Classify a sample as homogeneous or heterogeneous Classify a sample of matter as a pure substance or mixture based on the number.
Complex Fluids with Extended, Rigid Components Paul S. Russo, Louisiana State University, DMR The dynamics of rigid polymers, which is important.
Synthesis of diamond-like carbon films with super-low friction and wear properties A. Erdemir, O.L. Eryilmaz, and G. Fenske J. Vac. Sci. Technol. A 18(4),
Ferroelectric Nanolithography Extended to Flexible Substrates Dawn A. Bonnell, University of Pennsylvania, DMR Recent advances in materials synthesis.
Center for Materials for Information Technology an NSF Materials Science and Engineering Center Substrate Preparation Techniques Lecture 7 G.J. Mankey.
Controlled Self-assembly of Colloidal Cobalt Nanocrystals Yuping Bao, Michael Beerman and Kannan M. Krishnan Cobalt Nanocrystals Synthesis BF TEM image.
Chapter 3 (conclusion) Silica-containing materials X-ray diffraction Applications of single crystals Polycrystalline materials W.R. Wilcox, Clarkson University,
0-D, 1-D, 2-D Structures (not a chapter in our book!)
Chemistry XXI Unit 3 How do we predict properties? M1. Analyzing Molecular Structure Predicting properties based on molecular structure. M4. Exploring.
Effect of Cu(II) on the Aggregation of PolyNIPAM-co-Bypiridine Modified-Silica Nanoparticles Jean Remy Mutumwa* and William R. Seitz Department of Chemistry,
Department of Chemistry, Clemson University, Clemson, SC 29634
Classification of Matter Composition of matter Properties of matter.
Colloidal Polymerization of Ferromagnetic Nanoparticles into Cobalt Oxide Nanowires Jeffrey Pyun, University of Arizona, DMR We have developed.
U g e l s t a d L a b o r a t o r y UGELSTAD LABORATORY - group for surface, colloid and polymer chemistry ~30 persons Experimental and modelling group.
Date of download: 5/31/2016 Copyright © 2016 SPIE. All rights reserved. Characterization of upconversion nanoparticles surface-capped with amphiphilic.
Mukhtar Hussain Department of Physics & Astronomy King Saud University, Riyadh
Characterization of mixed films
Synthesis and Characterization of Magnetic ` Chitosan Microspheres for Medical Applications ` Sang Gil Ko 1 *, Jun Hee Cho 1, Yang kyu Ahn 1, Ki Chang.
COLLOIDAL SILICA. AGENDA Colloidal silica chemistry -Physical characteristics -Types Colloidal silica manufacturing -Evaporator -Grow/UF -UltraXol Colloidal.
 Sun Ha Lee1, Yangkyu Ahn1*, In Jung2, Keunwoo Lee2
RAMAN EFFECT.
Jun Hee Cho 1*, Sang Gil Ko 1, Yangkyu Ahn 1, Ki-Chang Song 2, Eun Jung Choi 3 1 Department of Nanochemistry & Biochemistry, Konyang University Nonsan,
Krakow, NGCM2004 Change of Co nanoparticles magnetic properties with oxidation Perov Nikolai.
R. Lucena Analytical Chemistry Department,
Results and Discussion Results and Discussion
Chemical synthesis of Peptide
Preparation of magnetic β - glucan microspheres by microemulsion method for targeting drug delivery system Jun Hee Cho 1*, Sang Gil Ko1, Yangkyu Ahn1,
3.3 Other types of microscopy
Synthesis and Characterization of ZnO-CdS Core-Shell Nanohybrids by Thermal Decomposition Method and Studies on Their Charge Transfer Characteristics Rama.
excellent contrast agents for imaging and labels for bioassays.
Ellie M. Papoutsakis, Rachel S. Riley, and Emily S. Day
Biological nanocomposite materials
Lecture 10: Nanodrug Design and Methods of Activation
OPTICAL TWEEZERS A bright shining tool Floor van de Pavert.
Do Now 10/18 WOD: DETER (di TUR) v.
Chemistry Unit 2 Classification of Matter
Separation of Mixtures
Separation of Mixtures
Introduction to Biophysics Lecture 17 Self assembly
Structural biology with carbon nanotube AFM probes
Gold Nanoparticles Gold nanoparticles are one type of metallic nanoparticle; others are Ni, and TiO2 nanoparticles. It has advantages over other metal.
Presentation transcript:

Composite Silica:Polypeptide Nanoparticles * 07/16/96 Composite Silica:Polypeptide Nanoparticles Sibel Turksen, Brian Fong & Paul S. Russo Macromolecular Studies Group Louisiana State University NSF, ACS, LSU Coates Fund Kasetsart University Bangkok, Thailand Thursday, November 18, 2004 *

Homopolypeptide Shell * 07/16/96 Fuzzballs a silica interior and synthetic homopolypeptide exterior. Silica (SiO2) core typically 200 nm diameter Optional superparamagnetic inclusion Homopolypeptide Shell typically 100 nm thick *

Why? The usual reasons for polymer-coated particles * 07/16/96 Why? The usual reasons for polymer-coated particles Stability studies, probe diffusion, standards, etc. The better reasons for polypeptide-coated particles Should allow excellent shell thickness control. Shell is rigid spacer for assembling silica spheres. Astounding chemical versatility and functionality, including chirality. Responsiveness and perfection of structures through reproducible helix-coil transitions. Easily attach antibodies for recognition of cancer cells, easily attach cancer-killing lytic peptides, too. When magnetic, good way to self-assemble all this functionality *

* 07/16/96 Our Little Corner of the World: Silica-Homopolypeptide Composite Particles Co-Si-homopolypeptide composite systems Hierarchical structures Homopolypeptide shell – PBLG, PCBL (can be helix as shown, or coil?) Superparamagnetic – Fe3O4 or Co core Mostly… unstructured, random coil polymers *

Silica-Stöber Synthesis Hydrolysis of tetraethyl orthosilicate (TEOS) C 2 H 5 O N 4 Si TEOS hydrolysis Stöber condensation

SEM & TEM of Silica Particles

Synthesis of Magnetite – Fe3O4

TEM- Silica Coated Fe3O4 Dark:Magnetic inclusions (~ 10nm) Gray:Glassy SiO2 matrix Magnetic silica particles

Superparamagnetic cobalt cit – + NH2(CH2)3Si(OH)3 NH2(CH2)3Si(OH)2O – Cit– Co N O Stöber reaction TEOS, APS, EtOH SiO2 OH – + H2O

TEM- Silica Coated Cobalt

Superparamagnetic Particles

Surface Functionalization

Homopolypeptides PBLG PCBL best understood homopolypeptide semiflexible structure helix-coil transition PCBL helix-coil transition @ 27 C in m -cresol

Synthesis of homopolypeptides

Summary: Particle Preparation cit – + NH2RSi(OH)3 N - SiO2- Cobalt particles CBL-NCA, monomer Superparamagnetic domain

Is the shell covalently attached? * 07/16/96 Is the shell covalently attached? Almost certainly (By the way, the polypeptide conformation is mostly a-helix with some b-sheet) *

* 07/16/96 TGA/DTA --Particles with ~ 23% by mass PBLG --Again, no evidence for binding of loose PBLG *

Dynamic Light Scattering * 07/16/96 Dynamic Light Scattering Bigger ones may diffuse slower (solvent viscosity effects) Flat plots indicate excellent, latex-like uniformity *

Particle Characteristics * 07/16/96 Particle Characteristics Silica Core Properties Radius from DLS: 97 nm Molar Mass: 4.5 x 109 Surface area: 15.6 m2/g PBLG Shell Properties 78 nm. ~90% solvent / 10% polymer. Polymer density limited by crowding around initiator sites. *

Not all initiators are active: crowding * 07/16/96 Unfortunately, the shell thickness was not controlled by [M]/[I]. Why not? Not all initiators are active: crowding Challenges: Controlling initiator density Attachment of ready-made polymers *

Helix-coil Transition of PCBL Matsuoka, M., Norisuye, T., Teramoto, A., Fujita, H. Biopolymers, 1973, 12,1515-1532

Early attempts showed NO change in the size of the particles—as if the shells were not responding. We reasoned this might be due to overcrowding on the surface.

3-(2-furoyl) quinoline-2-carboxaldehyde (ATTO-TAG™ FQ) Avoiding crowding 3-(2-furoyl) quinoline-2-carboxaldehyde (ATTO-TAG™ FQ) APTMS AEAPTMS MTMS NH2 25% amino groups

Silica-homopolypeptide Composite Particles DLS of Si-PCBL particles in DMF

Helix-coil transition of Co-PCBL

It’s Alive! This plot shows polydispersity

Hysteresis curve M -M Magnetization in opposite direction

SQUID- hysteresis plot of cobalt particles

SQUID- hysteresis plot of Co-PCBL

Formation of colloidal crystals ~ 0.5 m Sufficiently dense suspensions assemble into colloidal crystals. With a size that matches that of visible light, diffraction results. Domains with different orientations result in different and quite pure colors.

Colloidal Crystals (PCBL Shell) * 07/16/96 Colloidal Crystals (PCBL Shell) Sufficiently dense suspensions assemble into colloidal crystals. With a size that matches that of visible light, diffraction results. Domains with different orientations result in different and quite pure colors. Helical homopolypeptide shell *

Fun supramolecular synthesize & Applies to optical devices, Why Study? Beautiful! Fun supramolecular synthesize & characterize from nm to mm. Applies to optical devices, better lasers, pigment-free paint, “smart colloids”, artificial muscle, separations technology

Spectroscopic analysis of the crystal / nm 400 500 600 700 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 593 nm 568 nm 615 nm Transmittance measured on monochromator-equipped microscope Intensity FWHM of line is ~ 16 nm, comparable to typical interference filters

* 07/16/96 Achieving population inversion gets progressively harder for shorter wavelengths; lgreen < lred. E2 A12 B12 E1 l l *

Conclusions Facile synthesis & excellent uniformity Responsive shell * 07/16/96 Conclusions Facile synthesis & excellent uniformity Responsive shell Hierarchical structures, conformal transitions Potential applications —optical devices, stationary phases for chiral separation, model particles, artificial muscles, medical treatments Infinite variation with polypeptide chemistry *

Future work Helix-coil transition effect on magnetization Crosslinking particles Asymmetric particles Application of different grafting techniques Vapor deposition Grafting onto Controlling cobalt chains-rods Investigation of colloidal crystals Particles as probe diffusers

Crosslinking

Silica coating N NCA-monomer crosslinking Surface Functionalization

HELIX COIL N N N N N N N N N N N N N N N N N N N N N N N N N N N N N N