Size Exclusion Chromatography * Purification of nucleic acids and proteins Desalting Buffer exchange Dye terminator removal * = Gel Filtration

Size Exclusion Chromatography Principle: 1. larger molecules pass the matrix faster 2. small molecules, which can enter the pores of the matrix particles, migrate slowly

Size Exclusion Chromatography column preparation 2. column equilibration 3. sample application Volumes available: 1 ml, 200 µl, 15 µl [96 well plates] 4. elute fractions

Size Exclusion Chromatography Excellent performance of DextraSEC Columns Comparison of e.g. the removal of ammonia from oligonucleotides NAP 10 Column (GE Healthcare) DextraSEC NA10 AppliChem Higher purity within a smaller elution volume

Size Exclusion Chromatography AppliChem's products available: AppliXchange-G25 M DextraSEC Columns DextraSEC Plates

Size Exclusion Chromatography AppliChem's sales arguments: over 90 % recovery high chemical stability high resolution easy-to-use best price – best value for money

Size Exclusion Chromatography A Survey of the market: (1) Matrix substances Supplier Matrix Amount Price* AppliChem Dextran-based matrix: AppliXchange G25 M 100 g € 192,60 Sepharose-based matrix Illustra Sephadex G-25 GE Healthcare 100 g € 203,- BioRad Polyacrylamide-based matrix Bio-Gel P-6DG Gel 100 g € 269,- *: acc. to Euro price lists 2009/10

Size Exclusion Chromatography A Survey of the market: (2) ready-to-use columns Supplier Name Amount Price* AppliChem DextraSEC NA10 50 columns € 255,- GE Healthcare illustra NAP 10 50 columns € 299,- Econo-Pac 10DG 30 columns € 340,- BioRad *: acc. to Euro price lists 2009/10

Size Exclusion Chromatography Literature