Enzymes PP 35-37, 57

 Energy: the ability to do work  Requires energy to start the reaction (activation energy, E a ) Chem Rxns Review

 End in –ase (i.e. synthase, polymerase, helicase)  Reduces the activation energy (E a )  Protein catalyst

Each enzyme specific to a chemical rxn Helps assemble the product OR disassemble the reactants Changes shape when attached to the substrate (reactants) Not used up in the rxn, can be used over and over

 Every reaction has reactants that yield products Reactants  Products  Potatoes contain an enzyme called catalase which can break down hydrogen peroxide 2H 2 O 2  2H 2 O + O 2  How do you know it was working?

SUMMING UP YOUR OBSERVATIONS  Temp increase activity only to a certain point  Call this optimal temp  Temp changes the shape of the protein (enzyme)

SUMMING UP YOUR OBSERVATIONS  Enzymes have a specific pH they can work within  Too low or too high and activity slows or even stops

 Concentration  More enzyme  more work and more product  Point of saturation: enzymes can only work so fast even if you add more substrate  Max out on activity