Cigarette Smoke Extract Suppresses Human Dendritic Cell Function Leading to Preferential Induction of Th-2 Priming 高丰光 Robert Vassallo The Journal of Immunology,

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Cigarette Smoke Extract Suppresses Human Dendritic Cell Function Leading to Preferential Induction of Th-2 Priming 高丰光 Robert Vassallo The Journal of Immunology, 2005, 175: 2684– 2691.

Background Dendritic cells (DC) are key regulators of immune responses. we hypothesized that cigarette smoke- induced aberrance in DC function is an important mechanism by which smokers develop cancer, infection, and allergy— diseases common in smokers.

Methods Preparation of CSE Dendritic cell preparation and effect of CSE on dendritic cell maturation Mixed lymphocyte reaction Measurement of cytokines Flow cytometry and assessment of cellular viability Prostaglandin E2 determination and intracellular flow cytometry

Results CSE inhibits DC-induced T cell proliferation CSE-modulated DCs selectively inhibit Th-1 and enhance Th-2 cytokine production from T cells CSE suppresses IL-12 and enhances IL-10 release by maturing DCs CSE does not alter immature DC phenotype, but suppresses maturation-associated costimulatory and chemokine receptor expression CSE-induced suppression of DC function is not a consequence of cell death CSE enhances PGE2 release by LPS-matured DCs through induction of COX-2

Conclusions cigarette smoke extract (CSE) inhibits DC-mediated priming of T cells, specifically inhibiting the secretion of IFN-gamma whereas enhancing the production of IL-4 in the MLR. Conditioning with CSE did not effect cytokine (IL- 10, IL-6, or IL-12) production from im-DCs, but inhibited IL-12p70 release by LPS-matured DCs. In contrast,IL-10 secretion by LPS-activated CSE- conditioned DCs was enhanced when compared with control DCs. CSE also induced cyclooxygenase-2 protein levels in maturing DCs and significantly augmented endogenous PGE2 release.

Conclusions Conditioning of DCs with CSE also suppressed LPS-mediated induction of CD40, CD80, and CD86, and suppressed maturation-associated CCR7 expression. CSE has been reported to induce apoptosis of fibroblasts and epithelial cells, the immunomodulatory effects observed with CSE were not due to diminished DC viability. The effects of CSE on DC function were not exclusively mediated by nicotine, because equivalent, or even higher concentrations of nicotine than those found in CSE, failed to suppress DC-induced T cell priming. These data provide evidence that soluble components extracted from cigarette smoke suppress key DC functions and favor the development of Th-2 immunity.