3H-thymidine Tritiated (3H) thymidine.

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Presentation transcript:

3H-thymidine Tritiated (3H) thymidine

3H-thymidine Original method for measuring cell proliferation Radioactive Not compatible for multiplexed analyses

BrdU Br Br Br BrdU (5-bromo-2'-deoxyuridine) Br

BrdU Br Br Br Br

BrdU Br Br Acid or DNase Br Br

BrdU Br Br Br Br

BrdU Br Br Non-radioactive Multiplex compatible but, strand separation requirement for anti-BrdU access, can affect: Ability for other antibodies to bind Morphology Ability for dyes that require dsDNA to bind efficiently, i.e., cell cycle dyes Br Br

Click-iT™ EdU EdU (5-ethynyl-2'-deoxyuridine)

Click-iT™ EdU

Click-iT™ Edu Non-radioactive No DNA denaturation required Simplified protocol Small molecule detection Multiplex compatible, including Other antibodies Dyes for cell cycle analysis

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