KES 2004 Plasmid-DNAgram: Anagram Solving by Molecular Computing Based on GFP-Expressing Plasmid DNA 김수동3 박형주2 이승희2 이은석3 강봉균2, 3 장병탁1, 3 1School of.

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Presentation transcript:

KES 2004 Plasmid-DNAgram: Anagram Solving by Molecular Computing Based on GFP-Expressing Plasmid DNA 김수동3 박형주2 이승희2 이은석3 강봉균2, 3 장병탁1, 3 1School of Computer Science & Engineering, SNU 2School of Biological Sciences, SNU 3Interdisciplinary Program in Cognitive Science, SNU

Introduction (1) Biosequence exegesis Charles Lutwidge Dodgson (a.k.a. Lewis Carroll) Mathematician His fascination: Linguistics, Symbolic Logic, Number Theory, Cryptography, Probability Theory, & Geometry The author of Alice books Sequence Structure Function Role Lexicon Syntax Semantics Fragmatics

Introduction (2) Plasmid-DNAgram FRET (Fluorescent Resonance Energy Transfer) BFP-GFP pair CFP-YFP pair GFP-RFP pair (?) Cameleon FRET Cameleon : chimeric protein (CFP-CaM-M13-YFP) [Ca2+] : CaM-M13

Biomolecular FRET for Plasmid-DNAgram

Anagram “Rearrange the letters of ‘nor do we’ to make one word.” ‘Herlock Sholmes’ vs. ‘Sherlock Holmes’

Plasmid-DNAgram

Fluorescent Resonance Energy Transfer Förster equation

Biomolecular FRET

Cameleon FRET

Cameleon FRET for Plasmid-DNAgram Sequence Structure Function Role Lexicon Syntax Semantics Fragmatics GFP CFP/YFP FRET [Ca2+]

Biomolecular FRET for Plasmid DNAgram HEK293T cell CFP FRET YFP