B:S4NM The rAmylase Project Kits

Slides:

Advertisements

Similar presentations

Restriction Analysis of Plasmids

Advertisements

5 Stages involved in GE Isolation Cutting Ligation and Insertion

Enzyme Adventures Ellyn Daugherty San Mateo Biotech Career Pathwaywww.SMBiotech.com EMC/Paradigm Publishingwww.emcp.com/biotech.

Dolly the sheep ( ) 1. Animal and human cloning 2. Gene cloning.

Verify recombination by electrophoresis. Digest of rfp gene. Transform bacteria with recombinant plasmid. Recombination (ligation) of plasmid and rfp gene.

Chapter 4: recombinant DNA

Faster, Better Biotech: Mini-Preps

Introduction to ELISA ELISA – short for enzyme- linked immunospecific assay A technique that measures the amount of a specific protein in a solution.

Faster, Better Biotech: The rAmylase Project Lab 8g Mini-Prep of pAmylase 2014 Kit Ellyn Daugherty Simon Holdaway.

B:S4NM The rAmylase Project Kits Ellyn Daugherty Simon Holdaway The Loomis Chaffee School

Genetic engineering Genetic engineering: manipulating DNA or organisms to perform practical tasks or provide useful products We’re going to look at the.

Synthetic Biology Crash Course DAY ONE. Pre-Assessment 1:00-1:10.

Plasmid Isolation RET Summer Overall Picture Plasmid Isolation Remove plasmid pBS 60.6 from DH  E. coli.

Enzyme Adventures – Amylase Ellyn Daugherty San Mateo Biotech Career Pathway  Complete the card in the tri-fold.

AP Biology Biotechnology today  Genetic Engineering  manipulation of DNA  if you are going to engineer DNA & genes & organisms, then you need.

Genetic Engineering. We can use a process called gel electrophoresis to separate the pieces.

Biotechnology Techniques How to make Recombinant DNA Gel Electrophoresis PCR Summarize: What is this technique? Draw and label a diagram to show this technique.

Genetic Engineering changing DNA within an organism.

DNA Technology- Cloning, Libraries, and PCR 17 November, 2003 Text Chapter 20.

Chapter 20~DNA Technology & Genomics. Who am I? Recombinant DNA n Def: DNA in which genes from 2 different sources are linked n Genetic engineering:

A 3-week Biotech Unit for Any Biology Class Ellyn Daugherty SMBCP, San Mateo CA Educator/Author

Silly Lab Tech Which plasmid is it??. Your mission… Your silly lab tech did not label tubes of purified plasmid DNA that you need for your experiments!!

The rAmylase Project Faster, Better Biotech Ellyn Daugherty SMBCP, San Mateo CA

Genetic Engineering. What is genetic engineering? Application of molecular genetics for practical purposes Used to – identify genes for specific traits.

Biotechnology Lab Bio 11 Week 1.

Genetic Technologies Manipulating & Cloning DNA.

Molecular Genetics Lab Review. Bacterial Transformation Genetic transformation—host organism takes in and expresses foreign DNA Genetic engineering—manipulation.

Faster, Better rAmylase Project Ellyn Daugherty SMBCP, San Mateo CA

Biotech Lab #1 -Extraction Extract DNA from an organism’s cell to get the GOI – gene of interest.

Overview Amgen Biotech Labs In this set of labs, students will:

Genetic Engineering. Some New Words Genetic engineering Recombinant DNA: DNA that has been made by COMBINING two or more different organisms. Stanley.

Flash Cash “DNA is the Flash but Proteins are the Cash of Biotech ” Ellyn Daugherty SMBCP, San Mateo CA

 What is it?  What are they?  What is it?  How does it work?  DNA is isolated  DNA is copied with PCR  Cut with restriction enzymes  Run through.

Chapter 20: Terms to Know Genetic engineering Biotechnology

GENETIC RECOMBINATION By Dr. Nessrin Ghazi AL-Abdallat Lecturer of Microbiology.

AP Biology Biotech Tools Review AP Biology Biotech Tools Review  Recombinant DNA / Cloning gene  restriction enzyme, plasmids,

B:S4NM The rAmylase Project Kits Colin Heath G-Biosciences Ellyn Daugherty Lab 6c Assaying.

8.1 - Manipulating & Cloning DNA

Ellyn Daugherty SM Biotech Career Pathway Direct ELISA of Alpha-Amylase.

Gene Technology. What Is Gene Technology? Gene Tech  the alteration of genes inside a living organism to produce a specific results Examples? Examples?

Recombinant DNA Techniques chapter 18 Part I techniques and their applications. 1. Restriction Digest (to be done in lab) 2.Southern Blot 3.Northern.

In the pGLO lab, we will: Use recombinant DNA Genetically engineer E. coli bacteria by inserting a plasmid Plate and grow bacteria Determine if the proteins.

Vocab review Unit 8 - biotechnology. 1. Organism that has acquired genetic material by artificial means.

1. isolated mRNA for protein A from rat liver 2. reverse transcribed mRNA to DNA 3. added “sticky ends” to create an insert 4. ligated the insert (rat.

Molecular Cloning. Definitions   Cloning :   Obtaining a piece of DNA from its original source (Genome) and introducing it in a DNA vector   Sub-cloning:

If = Hypothesis, Then = Prediction Prediction= What you think the outcome of a specific experiment will be Hypothesis: Why If /then statement is NOT A.

Biotechnology: Science for the New Millennium Lab 9c Using Ion Exchange Chromatography to Separate Proteins Ellyn Daugherty SMBCP, San Mateo CA

- making changes to the DNA code of a living organism.

Developing ARG1 and ODC Standards for a Microfluidic ELISA Device By Kevin Grauberger Under direction of Dr. Debashis Dutta Chemistry Funded by EPSCoR.

Ellyn Daugherty Biotechnology; Science for the New Millennium, 2017 Text with STEM focus Laboratory.

pGLO™ Transformation and Purification of

Aim: What are some applications of Genetic Engineering?

Proteins are the Cash of Biotech

Proteins are the Cash of Biotech

Recombinant DNA Techniques chapter 19

DNA Tools & Biotechnology

Biotech Tools Review

Chapter 9: Biotechnology and Recombinant DNA

the manipulation of living organisms for human use Chapter 13

Recombinant DNA Techniques chapter 19

Recombinant DNA Techniques chapter 18 begins page 510

DNA Tools & Biotechnology

PGLO Lab Purpose: To transform E. coli bacteria by adding plasmids that allow the bacteria to glow green under UV light in the presence of arabinose sugar.

Determine the Identity of Unknown Plasmids

GENETIC ENGINEERING Human Cell DNA 1 Isolation

Cloning a DNA segment from bacteriophage

Genetically Modified Organisms

Material for Quiz 5 from Chapter 8

Abridged Genetic Engineering Pathway (Original “A” Sequence)

DNA Spooling with a Twist

Presentation transcript:

B:S4NM The rAmylase Project Kits Lab 8b Restriction Digestion to Verify pAmylase Plasmid Kit # BTNM-8b While you wait: Fill in the card in the book promo tri-fold for a copy of free curriculum and for the drawing at the end of the workshop to win a kit. Colin Heath G-Biosciences cheath@gbiosciences.com Ellyn Daugherty ellyn@BiotechEd.com www.BiotechEd.com

commercially important biotech product Amylase is a commercially important biotech product Amylase is an enzyme that catalyzes starch digestion to sugar. Used to: Remove starch in products Produce sugar from starch Processing of cellulostic biofuel

The goal of The rAmylase Project is to Learn how to assay for amylase, a protein of commercial interest (amylase) Genetically engineer cells to produce amylase (using pAmylase2014) Grow transformed cells to volumes where amylase is purified and assayed

The rAmylase Project Curricular model of rDNA/protein Business Chapters 1-5 Basic SLOP Lab 5f Characterizing Proteins by PAGE Lab 6c Assaying for Amylase Activity Lab 6d Direct ELISA of Bacterial Amylase Lab 6e Western Blot to Identify Amylase Lab 7g Amylase Concentration Assay Lab 8b pAmy Plasmid Restriction Digest Lab 8c pAmylaseTransformation of E. coli Lab 8g Miniprep for pAmylase Isolation Lab 9c Amylase Ion-Ex Chromatography Lab 13h Amylase Gene PCR

How do the restriction digestions look? Do the bands on the gel show that the sample DNA has only one each of the HindIII and BamHI restriction sites? How does this confirm the presence of pAmylase2014? .

Biotech Curricular Support www.BiotechEd.com PPTS, Syllabi, Activities, Hints, Links, etc. gbiosciences.com B:S4NM materials and kits emcp.com/biotech B:S4NM curriculum