Dr. Ferenc Horváth Dr. Barnabás Wodala

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Presentation transcript:

Dr. Ferenc Horváth Dr. Barnabás Wodala Preliminary photosynthesis measurements of selected wheat cultivars prior to inoculation Dr. Ferenc Horváth Dr. Barnabás Wodala

The LI-6400 Portable Photosynthesis System

The LI-6400 Portable Photosynthesis System

The LI-6400 Portable Photosynthesis System

An open system In an open system, photosynthesis and transpiration are computed from the differences in CO2 and H2O between in-chamber conditions and pre-chamber conditions.

Schematic without a CO2 mixer

Schematic with a CO2 mixer

IRGA (InfraRed Gas Analizator) CO2 measurement: 4,26 μm H2O measurement: 2,59 μm Infrared light source (wide spectrum) Gas inlet Gas outlet Filter Measuring box Detector Signal processing Indicator

Matching

Incoming concentrations must be stable breath, for example, is typically 30 000 μmol CO2 (mol air)-1 Ambient air contains 360 μmol CO2 (mol air)-1

Chlorophyll a fluorescence Light Reflected light Mehler reaction ETR PSII PSI ΦPSII Photosynthesis Transmitted light Photorespiration Chlorophyll a fluorescence Heat dissipation

preliminary measurements Setting up the instrument (Licor 6400, an open path gas exchange system) 4 selected wheat genotypes (1 week before the infection) PARout: sunny, depends on angle, 600 – 1800 µmol photon m-2 s-1 PARin: fixed, 1000 µmol photon m-2 s-1 (10% blue, 90% red) Tair: 27-29 °C Tleaf: 25-27 °C

Calculated parameters

Thank you for your attention!