Week 1: Orientation Orientation Biological safety Chemical safety

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Presentation transcript:

Week 1: Orientation Orientation Biological safety Chemical safety Fire safety Lab glassware Pipetting

MEDT 251 Goals Schedule Faculty Policies NiceNet class Objectives: cognitive, psychomotor, affective Offices and faculty Resources

Pipets Transfer fluids Make dilutions Make primary and secondary standards Reconstitute reagents and controls

Why so many kinds?

Pipet Variation… Types of fluid Degree of accuracy needed Blood, plasma, serum Aqueous reagents Viscosity Degree of accuracy needed Accuracy vs. efficiency Amount of fluid

Markings on Pipet Total volume and increments Calibration temperature: 20oC TD: to deliver TC: to contain Tolerance limits

Etched band means “blow out” Color bands just indicate size

General Types of Pipet Volumetric: for accuracy Volumetric transfer Ostwald Graduated: for efficiency Serological Mohr

Pipetting Select the right pipet Check for broken tip Aspirate with bulb Wipe the tip Adjust meniscus Deliver along side of vessel Place in wash receptacle

Checking…

No!

Tip is completely in the fluid Wipe the tip after aspiration

Dispense fluid along side of vessel

Division Safety Rules Read the Division’s safety manual Sign the Assumption of Risk and Release form

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