ControlDrug treated 2D gel images of bacterial protein sample Set of gel images for analysis.

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ControlDrug treated 2D gel images of bacterial protein sample Set of gel images for analysis

Steps followed in gel analysis 1) Set up Project 2) Detect and Quantify Spots 3) Analyze Expression Profiles 4) Present Results

ANALYSIS, RESULT AND DATA INTERPRETATION

Project Name Bacterial sample 2D-Gels for Analysis. Project Creation Date …………………………. Author …………………………. Description Bacterial sample Control vs Drug treated Use internal Standard No Pool C\Users\XYZ Groups Control (1 image) 780spots transferred from fused image 96 spots cancelled Drug Treated (1 image) 780 spots transferred from fused image 96 spots cancelled Vector Matching : Project Summary:

Profile Spot Information Control Totarol treated Spot ID : 123 pI : 5.26 MW : 65.1k Da Spot ID : 2082 pI : 5.28 MW : 44.8k Da 2. Spot details Control Totarol Fused 3D view

pI index Molecular weight (kDal) 3.Figure showing Molecular weight to pI 85 pI : ~4.53 Mol. weight : 24.9 kDal pI : ~4.90 Mol. weight : 75.2 kDal

No. of spots showing up regulation Up regulation No. of folds (and above)No.of spots Differential expression : Up regulation Up regulation x Folds or above

4.2. expression profile: Up regulation(8-10 folds up) -10 spots Mean normalized value of group-Control Mean normalized value of group 2 (Drug Treated) Ratio = ABCDE FGHIJ

No. of spots showing up regulation Down regulation No. of folds (and above)No.of spots Differential expression : Down regulation Down regulation x Folds or above

4.4.Expression profile: Down regulation(5-6 folds down) -10 spots ABCDE FGHIJ Mean normalized value of group-Control Mean normalized value of group 2 (Drug Treated) Ratio =

Hierarchical Cluster Analysis HCL Tree 5.Cluster Analysis

6. Scatter plot