Microscope Biology 101 Ms. Miller.

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Presentation transcript:

Microscope Biology 101 Ms. Miller

Types of Microscopes Light microscope Stereoscope Compound lenses to magnify objects. Lenses refract light to make object appear closer. 40x, 100x, 400x Stereoscope Allows binocular viewing of larger specimens.

Types of Microscopes Scanning Electron Microscope Use electrons to magnify objects instead of light waves. Can magnify up to two million times. Transmission Electron Microscope Uses electrons as well. Electrons are passed through very thin specimens.

Lenses Three magnification lenses Scanning Low power High power 4x magnification Low power 10x magnification High power 40x magnification

Lenses Ocular lens In addition to the magnification lenses. 10x magnification

Magnification To find the total magnification multiple the magnification of the lens by the magnification of the ocular lens. Magnification Ocular Lens Total Magnification Scanning 4x 10x 40x Low Power 100x High Power 400x

Focusing Always start with the scanning objective. Coarse knob to focus. Once you’ve focused on scanning, switch to low power. Coarse knob to refocus. Switch to high power. Fine knob to refocus.

Focusing Can’t see the specimen? See a line? Is it too light or too dark? Adjust the diaphragm. See a line? It is a pointer Useful for pointing things out to partner or teacher. Try twisting the eyepiece

Drawing Specimens Use pencil. Drawing should be large enough to see detail. Label items in drawing. Write what magnification you are viewing the object at. Label the object.

Drawing Specimens Cell Membrane Ameba (400x) Nucleus

Clean Up & Storage Make sure the scanning lens is in place. Wrap cords around the base. Cover microscope. Wash and dry slides before placing them back in slide box. Cover slips are to be thrown away.