Analytical methods Houdková Chocholáčová

Analytical methods צ Absolute analytical methods (direct evaluation of the analytical signals with regard to the analyte concentration) צ Relative analytical methods (which use calibration function, sample standards are obligatory) ﮏ Gravimetry ﮏ Volumetry (titration methods) ﮏ Instrumental methods – CZE – GC

Electromigration methods use motion of ionized particle in the electrical field velocity motion is directly proportional to their charge and size

Main variants of the electromigration methods Capillary Zone Electrophoresis (CZE) Capillary Gel Electrophoresis (CGE) Micellar Electrokinetic Capillary Chromatography (MECC) Capillary Electrochromatography (CE) Capillary Isoelectric Focusing (CIEF) Capillary Isotachophoresis (CITP)

CZE CZE separation and determination of cations, anions and components without charge INSTRUMENTATION אCapillary אSource: 20 – 30 kV אPlatinum electrodes אVials with buffer solutions אDetectors אTime (such 10 minutes) אElectroforetogram

Capillary ک size: 60 cm x 75 µm ک silica capillary covered by polyamide ک the whole capillary contains 10 µl, sampling volume is 10 nl ک various variant of sampling (hydrodynamic, electrokinetic)

Principle of CZE ﯼ method for separation and determination of cations, anions and so components without charge on the basis of diferent electrophoretic movement ﯼ sample at start → insert electrical field → components A,B are moving with diferent constant speed towards to electrode → origin zone ﯼ to origin electroosmotic flow (EOF) and his impact on separation and shape peaks

Application ﺏ determination of dye at foodstuff ﺏ monitoring composition of foodstuff during the production and storage ﺏ pollution ﺏ analyse of DNA (genetic engineering) ﺏ and so, and so, and so …

Reference Ж website Ж Analytická chemie, Lumír Sommer (textbook) Ж laboratory from ISA