Dual regulation of Snail by GSK-3 -mediated phosphorylation in control of epithelial–mesenchymal transition Binhua P. Zhou1 et al., Nature Cell Biology,

Slides:

Advertisements

Similar presentations

[Vierstra, 2003 TIPS].

Advertisements

Tyrosine kinase signalling (review) TGF signalling Notch signalling.

Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm Lindsley R.C. et al, Development (2006)

Inhibitory Gli3 Activity Negatively Regulates Wnt/  -Catenin Signaling Ulloa F. et al, Current Biology (2007)

1. Epithelial Mesenchymal Transition ( EMT ) 2 3.

Wnt/β-catenin Signaling Pathway Prospective Target for Cancer Treatment Emelia E Conte 1, Jun Yin 2, Mei Zhang 2 Western Blot Introduction.

Relevant KEGG pathways Additional file 2 Te Pas et al. The file shows more KEGG pathways as found by the PERL script search of the database.

Purified Wnt 5a protein activates or inhibits β-catenin-TCF signaling depending on receptor context Mikels et al., 2006, PLOS Biology.

Date of download: 6/21/2016 Copyright © 2016 McGraw-Hill Education. All rights reserved. The hedgehog (Hh) signaling—a proliferative pathway especially.

Cell Physiol Biochem 2017;42:2582– DOI: /

Nithya Krishnamurthy, Razelle Kurzrock Cancer Treatment Reviews

A. B. C. D E. F. 100 GSK3β pGSK3β pAkt Akt p38/ SAPK

Strategies for differential inhibition of mTORC1 and mTORC2 with rapamycin. Strategies for differential inhibition of mTORC1 and mTORC2 with rapamycin.

David Largaespada, Nancy Ratner Cancer Cell

A Signal Transduction Pathway from TGF-β1 to SKP2 via Akt1 and c-Myc and its Correlation with Progression in Human Melanoma Xuan Qu, Liangliang Shen,

Connecting COX-2 and Wnt in cancer

MAPK pathway inhibitors.

IRS-1: Auditing the effectiveness of mTOR inhibitors

Wild-Type RAS: Keeping Mutant RAS in CHK

Small-molecule inhibitor QLT-0267 suppresses ILK activity and inhibits its downstream signaling. Small-molecule inhibitor QLT-0267 suppresses ILK activity.

Figure 1 A schematic representation of the HER2 signalling pathway

Nat. Rev. Urol. doi: /nrurol

Chadrick E. Denlinger, MD, John S. Ikonomidis, MD, PhD, Carolyn E

Nat. Rev. Urol. doi: /nrurol

Nat. Rev. Clin. Oncol. doi: /nrclinonc

Methed-Up FOXOs Can't In-Akt-ivate

Nat. Rev. Nephrol. doi: /nrneph

Caught in the Akt: Regulation of Wnt Signaling in the Intestine

Figure 1 WNT signalling pathways

PHLPPing It off: Phosphatases Get in the Akt

Volume 142, Issue 4, Pages (August 2010)

A Liver Full of JNK: Signaling in Regulation of Cell Function and Disease Pathogenesis, and Clinical Approaches Ekihiro Seki, David A. Brenner, Michael.

PKB Binding Proteins Cell

p27 as a target for cancer therapeutics

The Ying and Yang of Bacterial Signaling in Necrotizing Enterocolitis

IRS-1: Auditing the effectiveness of mTOR inhibitors

Wnt/β-catenin signaling and kidney fibrosis

Killing Lymphoma with Smac-Mimetics: As Easy as ABC?

Distribution of the phosphoproteins based on GO analysis, including biological process (Left) and cellular component (Right). Distribution of the phosphoproteins.

Control of Smad7 stability by competition between Acetylation and Ubiquitination Gronroos et al., 2002, Mol Cell.

Trop‐2 can bind to IGF‐1 and interfere with IGF‐1R signalling.

Advances in Hypoxia-Inducible Factor Biology

Erik G. Huntzicker, Anthony E. Oro

DUB-le Trouble for Cell Survival

p38 MAPK activation is required for phosphorylation of Akt at Ser473.

Wnt/β-Catenin Signaling in Development and Disease

Volume 17, Issue 2, Pages (February 2013)

Long Zhang, Fangfang Zhou, Peter ten Dijke

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities

Model of translational regulation by PDGFRα.

Some major pathways controlling protein breakdown in skeletal muscle.

A summary of CCM3-mediated signalling pathways implicated in CCM formation. A summary of CCM3-mediated signalling pathways implicated in CCM formation.

The rudimentary human β-cell mitogenic signaling road map.

The Akt-GSK3β and canonical NF-κB pathways are activated in Slc7a5-deficient osteoclasts. The Akt-GSK3β and canonical NF-κB pathways are activated in Slc7a5-deficient.

Cell Migration: GSK3β Steers the Cytoskeleton's Tip

Regulation of p53 by MDM2. p53 and MDM2 form an autoregulatory feedback loop. p53 stimulates the expression of MDM2; MDM2, in turn, inhibits p53 activity.

Phases of the Cell Cycle

Nat. Rev. Urol. doi: /nrurol

Phases of the Cell Cycle

Schematic view of intracellular signaling cascades triggered by receptor tyrosine kinase in Schwann cells. Schematic view of intracellular signaling cascades.

Western blot analysis of Wnt signaling genes.

MiR-200c is a PI3K–AKT signaling pathway regulator in CRC

A. A. Honokiol inhibits TNF-induced NF-κB activation, IκBα phosphorylation, and IκBα degradation. Honokiol inhibits TNF-induced activation of NF-κB. H1299.

Potential model of HMQ1611 inhibiting breast cancer cell proliferation

Expression data from genes involved in regulation of transit through the cell cycle in response to treatment with E2 and OHT. A. Expression data from genes.

Phosphorylation Addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes,

Inhibition of the GSK3 activity attenuates the morphology and sphere-forming ability of iCSCs. Inhibition of the GSK3 activity attenuates the morphology.

Oncogenic potential of the extraribosomal functions of RPs

Regulation of signaling by wild-type and oncogenic Ras.

Turning Reciprocal Feedback Regulation into Combination Therapy

Presentation transcript:

Dual regulation of Snail by GSK-3 -mediated phosphorylation in control of epithelial–mesenchymal transition Binhua P. Zhou1 et al., Nature Cell Biology, 2004

P Snail GSK3-β P proteasome ?

III Snail GSK3-β ?

Myc- IP:Flag- I -MycIP: Myc + r-P 32 ATP GST-snail Snail GSK3-β P P

Stability Half-lives Snail P P P P P P

P P P P P

MCF7 Snail (-) Snail

P P P

Biochim Biophys Acta ① ②

EGF,IGF Akt, MAPK GSK -3β Snail E-cad EGF,IGF Akt, MAPK GSK -3β Snail E-cad X X X P P

EGF,IGF Akt, MAPK GSK -3β Snail E-cad X X P EGF,IGF Akt, MAPK GSK -3β Snail E-cad X P

* Wnt-dependent regulation of the E-cadherin repressor snail (JBC,2005) : Snail displays ß-catenin-like canonical motifs that support its GSK3ß-dependent phosphorylation, ß-TrCP-directed ubiquitination and proteasomal degradation. : Wnt signaling inhibits Snail phosphorylation and consequently increases Snail protein levels and activity, while driving an in vivo epithelial-mesenchymal transition that is suppressed following Snail knockdown.