혈액검사 면역표현형 검사 골수검사 세포유전학 특수혈액 분자유전학 일반혈액 혈액응고.

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Presentation transcript:

혈액검사 면역표현형 검사 골수검사 세포유전학 특수혈액 분자유전학 일반혈액 혈액응고

일반혈액 검사항목 CBC : RBC Hemoglobin(Hb) Hematocrit(Hct) 일반혈액 검사항목 CBC : RBC Hemoglobin(Hb) Hematocrit(Hct) RBC index : MCV, MCH, MCHC WBC Platelet Reticulocyte Eosinophil counts ESR Blood film : WBC differentil PB morphology Malaria parasites

특수혈액검사 혈액응고검사 Bleeding time Osmotic fragility Prothrombin time 특수혈액검사 혈액응고검사 Bleeding time Prothrombin time aPTT Thrombin test Fibrinogen Factor assay FDP test Paracoagulation test Antithrombin III Lupus anticoagulant Clot retraction time Clot lysis time Platelet function test Osmotic fragility Autohemolysis test Ham's test Sucrose hemolysis test Fetal hemoglobin LE cell test LAP score NBT test BM aspiration & biopsy Chromosome study BM cell special stain Molecular study Immunophenotyping

혈액검사용 검체채취 Equipment : Lancet, glass capillary Plastic syringe with needle Vacuum system Skin puncture : Capillary blood 3rd/4th finger : Blood smear Ear lobe : Bleeding time Venipuncture : antecubital vein 23-gauge ..... 3- 5ml 21-gauge ..... 5-10ml 20-gauge ..... 10-15ml 18-gauge ..... 30-50ml

신생아와 소아의 혈액채취 Capillary blood : heal or first toe Cord blood External jugular vein Femoral vein

항응고제 및 관련 혈액검사 EDTA ................................. 일반혈액검사 백혈병표지검사 분자유전학검사 Sodium citrate ................... 혈액응고검사, ESR Heparin Sodium Heparin ....... 특수혈액검사 Preservative-free Heparin ....염색체검사 세포배양 Defibrillation

혈액검체의 보관 혈액검체 저장시 변화 2시간 이상 검사지연 : 냉장보관 2시간내 검사 : ESR, PT 즉시 검체분리 : Cold agglutinin (혈장 실온보관) 혈액검체 저장시 변화 물리적 변화 : autoagglutination, plasma viscosity 증가 적혈구 : swelling, crenation, sphering 중성구 : vacuolation, nuclear swelling, loss of granules 림프구,단구: "clover-leaf" appearance with vacuolation

혈장과 혈청의 차이점 전혈 + 혈장 (plasma) 항응고제 전혈 혈청 (serum) Blood clot : RBC +WBC+ Platelet +Factor I, II, V, VIII

Different Layers of Venous Blood Plasma Total volume blood Platelets WBCs Buffy coat Reticulocytes RBCs

혈액성분별 검사내용 Plasma 혈액응고검사 특수혈액검사 Buffy coat smear 말라리아검사 악성세포검출

혈액은행 수혈혈액 Red blood cells (RBC) 200-250ml Fresh frozen plasma (FFP) 160-200ml Whole blood (WB) 400-450ml Platelet concentrate (PC) 40ml Platelet-rich plasma (PRP) 200ml Washed RBC (WRBC) 250ml Cryoprecipitate (CRYO) 30ml Anticoagulant : CPDA-1