Lecture 07 Transcription Based on Chapter 5 Gene Expression: Transcription 1/29/2016 Copyright © 2010 Pearson Education Inc.
1. Gene Expression—The Central Dogma: An Overview
2a. The Transcription Process
2b. The Transcription Process
3a. Transcription in Bacteria - Initiation of Transcription at Promoters
3b. Transcription in Bacteria - Initiation of Transcription at Promoters
4. Elongation of an RNA Chain
5. Termination of an RNA Chain
6. Transcription in Eukaryotes
7. Eukaryotic RNA Polymerases RNA polymerase I, located in the nucleolus, transcribes the three major rRNAs (28S, 18S, and 5.8S). RNA polymerase II, located in the nucleoplasm, transcribes mRNAs and some snRNAs. RNA polymerase III, located in the nucleoplasm, transcribes tRNAs, 5S rRNA, and the remaining snRNAs.
8. Transcription of Protein-Coding Genes by RNA Polymerase II
9. Promoters and Enhancers
10. Transcription Initiation
11a. The Structure and Production of Eukaryotic mRNAs
11b. The Structure and Production of Eukaryotic mRNAs
12. Production of Mature mRNA in Eukaryotes
13. 5’ Modification
14. 3’ Modification
15. Introns
16. Processing of Pre-mRNA to Mature mRNA
17. Coupling of Pre-mRNA Processing to Transcription and to mRNA Export from the Nucleus