K. Loens AZ KLINA 12/03/2015 NRC invasive β hemolytic streptococci not belonging to Group B: epidemiology 2009- 2014.

Slides:

Advertisements

Similar presentations

Antimicrobial resistance surveillance in Ireland Results of invasive Streptococcus pneumoniae infection (blood/CSF) surveillance (2009Q1-4) **** Data as.

Advertisements

Dangerous Dublin: The Virulent Salmonella Colette O’ Hare 1, Conor Burke 2, Suzanne Gray 3, Niall Delappe 1, Ger Doran 1, Grainne McHale 2, Dr. Gerard.

Cerebrospinal fluid Culture + Body Fluid Culture.

Susceptibility Testing Anaerobes

SURVEILLANCE FOR GLYCOPEPTIDE-RESISTANT ENTEROCOCCI Drs N Bosman, T Nana & C Sriruttan CMID NHLS Dr Charlotte Sriruttan SASCM 3/11.

Microbiological diagnosis of streptococcal pharyngitis: Lacunae and their implications  Presented by  Dr. Arifur Rahman  MPhil Student  Journal: IJMM,

Public Health Event Reporting: Lecture Template

Role of the laboratory in disease surveillance

Antimicrobial Susceptibility Testing – Part II

Phylogenetic analysis of E. coli causing bacteraemia in the UK and Ireland in 2001 and 2010 Williams, MJ.*, Doumith, M.*, Hope, R.*, Wain, J.*, Underwood,

Giebink – FDA – 01/2001 Otitis Media Epidemiology and Drug-Resistant Streptococcus pneumoniae G. Scott Giebink, M.D. Professor of Pediatrics and Otolaryngology.

Molecular Characterization of Invasive Group A Streptococci in Alaska Karen Rudolph, Ph.D. Arctic Investigations Program DEISS, NCPDCID, CCID,

National and International Spread of Antimicrobial Resistance: Clones and Genes Professor Alan Johnson, Health Protection Services - HPA.

Overview of Haemophilus influenzae and related bacteria Leonard Mayer, Ph. D. Chief, Meningitis Laboratory MVPDB/DBD/CDC 1.

NCCLS (1997) Approved standard M27-A. Rex JH et al. Clin Infect Dis (1997) 24: Pfaller M. A., et al. Diagn Microbiol Infect Dis (1999); 35:19-25.

The Connecticut Experience with non-O157 STEC “Seek and Ye Shall Find” Sharon Hurd, MPH October 17, 2007 Connecticut Emerging Infections Program FoodNet.

Panton-Valentine Leukocidin (PVL) is a toxin that destroys white blood cells and is a virulence factor in some strains of Staphylococcus aureus. PVL occurs.

Typhoid Fever in Africa: Emerging Flouroquinolone Resistance S KARIUKI 1,3, G REVATHI 2, J MUYODI 1, J MWITURIA 1, A MUNYALO 1, S MIRZA 3, CA HART 3 1.

Introduction for Basic Epidemiological Analysis for Surveillance Data National Center for Immunization & Respiratory Diseases Influenza Division.

In vitro antifungal activity of voriconazole and fluconazole against Candida spp. isolated from oral fluid Author: Tatarici Andreea Co-authors: Lecturer.

Genome-wide longitudinal analysis of emm1 invasive Group A Streptococcus isolated from Belgian patients during 1994 ˗ 2013 J. Coppens 1, B. B. Xavier,

Salmonella enterica Bredeney: Third commonest cause of human infection in Ireland in 1999 C. A. O’ Hare, M. Cormican. G. Corbett-Feeney, S. Fanning and.

Tracking Evolutionary Genetic Events in Group A Streptococcus Using Whole Genome Mapping J. Coppens 1, J. Sabirova 1, C. Lammens 1, K. Loens 2, S. Malhotra-Kumar.

Technology from seed Computer Assisted Analysis of Pulsed-field Gel Electrophoresis gels João André Carriço 40th ESCMID Postgraduate.

Chunyan Liu Beijing Children’s Hospital, Capital Medical University Adenovirus Infection in Children with Acute Lower Respiratory Tract Infections in Beijing,

Center for Communicable Diseases Center

Antimicrobial resistance and virulence genes among Streptococcus suis serotype 2 isolates from pig-derived products  Streptococcus suis is an emerging.

Correspondence between typing methods results: A web-based quantitative analysis tool Carriço, J.A.; F. R. Pinto; J. Melo-Cristino; H. de Lencastre ; J.

MALDI TOF analysis of Streptococcus pneumoniae from Cerebrospinal Fluid for the diagnosis of Acute Bacterial Meningitis Dr. R. Ravikumar, M.D., Professor.

Outbreak of USA300-like MRSA colonization in a postpartum maternal and neonatal care center in Korea Misuk Ji 1*, Heungsup Sung 1, Ai-rhan Kim 2, Mi-Na.

K. Loens, E. De Herdt, S. Verkroost, C. Lammens, M. Ieven and H. Goossens Surveillance of invasive Group A Streptococci in Belgium (01/01/ /12/2014)

How I deal with an outbreak? Prof Bertrand SOUWEINE Medical ICU Clermont-Ferrand France ISICEM March 2009.

Outcomes of Carbapenem-Resistant K. pneumoniae Infection and the Impact of Antimicrobial and Adjunctive Therapies Gopi Patel, MD; Shirish Huprikar, MD;

MRSA in People Who Inject Drugs: Estimating burden and risk factors Dr Maya Gobin – Consultant Epidemiologist.

TB – LABORATORY INVESTIGATIONS by Dr. Zubaidah Abdul Wahab Datin Dr. Ganeswrie Raj 1.

Molecular epidemiology of MRSA Epidemic and pandemic clones circulation in Romania Professor Lia Monica Junie Professor Lia Monica Junie, University of.

Microbiology Specimen Collection Guide For UCSF Operating Rooms

Research on Antimicrobial Resistance

Acknowledgments/Funding

Overview of Haemophilus influenzae and related bacteria

Number of S, I and R to six antibiotics

15th International Symposium on Staphylococci and Staphylococcal Infections 26th-30th August 2012 Lyon, France Prevalence and mechanism of resistance.

This poster will be made available for download after the meeting at :

Ontario Group A Streptococcal Study

C-672b First report of Group B Streptococcus Isolates with Cross-resistance to Lincosamides, Streptogramin A, and Pleuromutilins in the United States P.

Emergence of Klebsiella pneumoniae with an AmpC(DHA-1) and blaSHV-11 in a Belgian hospital. Timothy Vanwynsberghe1, Katia Verhamme2, Marijke Raymaekers3,

REDUCED RATES OF VANCOMYCIN RESISTANT ENTEROCOCCI (VRE) COLONIZATION

BARBARA KRUCSÓ 1, dr. MÁRIA GACS 2, dr

Prolonged postprocedural outbreak of Mycobacterium massiliense infections associated with ultrasound transmission gel A. Cheng, W.-H. Sheng, Y.-C. Huang,

Case Definitions: Reportable Streptococci (DRSP, GBS, GAS)

Carriço, J. A. ; F. R. Pinto; J. Melo-Cristino; H. de Lencastre ; J. S

Molecular characterisation of

Clinical and Microbiologic Characteristics of Group A Streptococcal Skin and Soft Tissue Infection in Children 謝旻玲1、沈靜芬1,3、蘇榆芳4、李冠賢1,3、何宗憲2、王世敏2、劉清泉1,3.

K. Loens1, N. Bossuyt2, H. Jansens1

Neonatal sepsis in Kilifi

NRC invasive β hemolytic streptococci not belonging to Group B: epidemiology K. Loens AZ KLINA 12/03/2015.

Carriage and antibiotic resistance of respiratory pathogens and molecular epidemiology of antibiotic-resistant Streptococcus pneumoniae colonizing children.

D.E. Low Clinical Microbiology and Infection

The role of fourth-generation cephalosporins in the treatment of infections caused by penicillin-resistant streptococci Keith Klugman, Fred Goldstein,

…monitoring trends in antimicrobial resistance in Canada

Comparison of a commercial and ‘in house’ assay for B19 DNA

C. Oprica, C.E. Nord Clinical Microbiology and Infection

C. Plainvert, A. Doloy, J. Loubinoux, A. Lepoutre, G. Collobert, G

Nationwide laboratory-based surveillance of invasive beta-haemolytic streptococci in Denmark from 2005 to 2011 L.M. Lambertsen, H. Ingels, H.C. Schønheyder,

…monitoring trends in antimicrobial resistance in Canada

Increasing prevalence of multidrug resistance among P

EARS-Net data on Antimicrobial Resistance in Ireland, Q

Diagnostic tools for intestinal pathogenic E. coli.

Multiplex PCR assay to detect common resistance determinants in B

The future TB clinic. The future TB clinic. Sputum, saliva, and fecal samples are collected from patients for genome sequencing, SNP typing, or metagenomic.

Presentation transcript:

K. Loens AZ KLINA 12/03/2015 NRC invasive β hemolytic streptococci not belonging to Group B: epidemiology

Role: NRC β-hemolytic streptococci not belonging to Group B Confirmation of identification S. pyogenes Since August 2014 also S. dysgalactiae and zoonotic Streptococci (eg. S. equi) Antibiotic susceptibility testing: MIC, Triple disc Erythromycine and tetracycline resistance genes Monitoring of circulating strains by typing Emm-typing Detection of virulence genes (SpeA, ssA and SpeC) Investigation of outbreaks PFGE Participation in international surveillance-activities Biobank

Yearnr of invasive strains received/total nr of strains received Nr of invasive strains received from labs submitting on a regular basis / / / / / / Invasive streptococcal strains received at the NRC ( )

Isolation site of invasive β-hemolytic streptococci not belonging to Group B Isolation site% blood74.0 Sterile wounds6.2 tissue4.2 Pleural fluid2.1 Other sterile sites (CSF, peritoneal fluid, synovial fluid, sterile not specified) 13.5

Antibiotic resistance in β-hemolytic streptococci not belonging to Group B ( ) YearPenicillins (%) Erythromycin R/I (%) Clindamycin resistance / inducible resistance (%) Tetracycline R/I (%) 2009 a 03.2/00/1.67.1/ b 02.1/01.4/0.77.0/ c 03.4/0.90.9/0.98.6/ /5.71.3/6.16.5/ * / * / a available for 187/192 strains b available for 143/195 strains c available for 116/197 strains * Norm EU-CAST

Emm-types invasive S. dysgalactiae ( ) YearEmm-types 2009*/ 2010*stG *stG652 (2x), stC74, stG643, stG *stC2sk 2013*/ 2014stC74, stG485, stG62647, stG643 *emm-typing only done for GAS associated with invasive infections common emm-types Kittang et al Eur. J. Clin. Microbiol. Infect. Dis. Loubinoux et al J. Clin. Microbiol. Oppegaard et al Eur. J. Clin. Microbiol. Infect. Dis.

Top 4 emm-types invasive GAS strains ( ) YearMost frequent (%) 2nd (%)3rd (%)4th (%) 2009emm1 (21.9)emm89 (13.5)emm3 (13.0)emm12 (7.8) 2010emm1 (16.4)emm28 (12.3)emm6 (9.2)emm3 (8.7) 2011emm1 (23.4)emm3 (12.5)emm28 (9.9)emm6 (7.8) 2012emm1 (20.9)emm3 (12.6)emm75 (10.2)emm89 (7.0) 2013emm1 (30.2)emm3 (13.5)emm89 (8.8)emm28 (6.5) 2014emm1 (19.5)emm89 (13.3)emm6 (6.6) emm75 (6.6) emm3 (6.2)

Invasive GAS in children <4 years Children (%)58/467 (12.4) 98/593 (16.5) 27/213 (12.6) 24/229 (10.5) 42/216 (19.4) 28/241 (11.6) Invasive strains (%) 28/58 (48.2) 27/98 (27.6) 27/27 (100) 24/24 (100) 37/42 (88.1) 25/28 (89.2) Emm-type most frequently detected (%) 9/28 (32.1) emm12 7/27 (25.9) emm1 6/27 (22.2) emm12 1/24 (4.2) emm1 19/37 (51.3) emm1 8/25 (32.0) emm1 Emm-type 2nd most frequently detected (%) 5/28 (17.9) emm1 5/27 (18.5) emm6, emm12 5/27 (18.5) emm1, emm4 5/24 (20.8) emm75 3/37 (8.1) emm3, emm12, emm89 5/25 (20.0%) emm4

Virulence genes emm 1, 2013 nr formulierregistratieSpeASpeBSpeCSpeFSsa mrt mrt mrt mrt mrt mrt apr apr apr apr apr apr apr apr apr apr apr apr apr apr mei mei mei mei mei mei

PFGE emm strains, same virulence genes PFGE type 3 already circulating in Belgium in 1993 (Descheemaeker et al J. Med. Microbiol.

most similar to M1 476, a Japanese sepsis isolate remarkable genomic stability over the last 20 years with minor genomic changes driven by prophages. Whole genome maps of emm1 invasive strains ( ): Longitudinal collection Coppens et al ICAAC. #B-2001

Fatal outcomes due to β-hemolytic streptococci not belonging to group B YearNumber (%)Emm-types involved (7.8)emm1 (n=6), emm3 (n=4), emm28 (n=2), emm4, emm5, emm77 (n=1) (5.6)emm3 (n=3), emm12 (n=3), emm87 (n=2), emm1, emm6, emm28 (n=1) (9.1)emm3 (n=5), emm89 (n=4), emm1 (n=3), emm6 (n=2), emm9 (n=1), emm12 (n=1), emm108, emm230 (n=1) (7.4)emm1 (n=8), emm3 (n=2), emm11, emm12, emm29, emm77, emm81, emm87 (n=1) (7.4)emm1 (n=6), emm3 (n=3), emm4, emm5, emm12, emm75, emm87, emm169, emm219 (n=1) (5.7)emm1 (n=6), emm6 (n=2), emm3, emm22, emm29, emm44, emm77, emm89 (n=1)

Remarks, conclusions and future analyses (1) So far no legal obligation for laboratories to submit invasive strains. Increasing number of GAS strains submitted to the NRC Registration forms not always completed. Helpfull if clinical data would be completed as well. Fatal outcomes based on info on registration forms <10% Emm1 clone was re-emerging in Belgium in 2013: Highly prevalent in children with invasive GAS (±50%) Limited number of S. dysgalactiae strains received so far Future analyses: GAS: Detection of larger number of virulence genes S. dysgalactiae: analysis of virulence genes

Remarks, conclusions and future analyses (2) For optimal surveillance the NRC needs YOU!