Live food aquaculture training course www.aquatrain.org Rotifer Biology and Strains.

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Live food aquaculture training course Rotifer Biology and Strains

Live food aquaculture training course Systematics Phylum: Nemahelminthes eller Aschelminthes Class: Rotifera (lat. Hjulbærer) Order: Monogononta Familiy: Branchionidae 90% are freshwater species

Live food aquaculture training course Morphology Corona: movements and generation of water currencts for ingestion of feedparticles up to 30  m Mastax: crushes the ingested particles Stomach with glands Unpaired gonads Primitive excretion system and bladder Pseudocoelom: function as a hydrostatic skeleton and cardiovascular system Common opening for bladder and uterus; cloaca Foot: the pedal has glands tha secreets glue for fastening to the susbstrate

Live food aquaculture training course Life cycle I Optimal environmental conditions Amictic females produce genetic idendical offspring from diploid eggs Usually one egg/female is observed Suboptimal environmental conditions (mixis stimulus) Mictoc female produce mictic (haploid eggs) from which males hatch Resting eggs are produced after fertilization of the mictic egg Hatching is initiated after stimulus (e.g temperature/salinity changes) Resting egg with amictic females Resting egg

Live food aquaculture training course Temperature and reproduction Effect of temperature on the reproduction activity of Brachionus plicatilis. (After Ruttner-Kolisko, 1972).

Live food aquaculture training course Population dynamics Specific growth rate: u = (lnN2 – lnN1)/t Instant production : P =  N Relation between growthrate (  ) and eggfrequency (ER):  = ER – 0,2 Expected production the next day: P = N(ER – 0,2)

Live food aquaculture training course Strain recognition SS-type S-type: lorica shows pointed spines L-type lorica shows obtuse angled spines SS

Live food aquaculture training course Rotifer strain information StrainLorica length (  m) Temperature range Fits for L-type (Brachionus plicatilis) °CLarvae until Artemia nauplii is small enough S-type (Brachionus rotundiformis) °CInitial feed for larvae with small gapes (sea brea) SS-type (small strain of S-type) 28-35°CLarvae with gapes < 100  m