HISTOLOGY REVIEW Bone Tissue Dr. Tim Ballard Department of Biology and Marine Biology

Human bone – ground – cross section -- India ink – 4x objective The arrowheads indicate osteons or Haversian systems. An osteon is that bony tissue surrounding and a part of a single Haversian canal. Compact bone This is a section of dried and long dead ground compact bone. The living cells are gone, leaving behind spaces that can be back-filled with India ink.

Human bone – ground – cross section -- India ink – 10x objective Interstitial lamellae are layers of old bone tissue from an osteon that is bring destroyed by remodeling. Compact bone osteon interstitial lamellae

Human bone – ground – cross section -- India ink – 20x objective Each osteon runs more-or-less parallel to the long axis of the bone. If you see a canal that is perpendicular, it is a Volkmann’s canal. Compact bone Haversian canal osteocytic lacuna lamellae interstitial lamellae

Human bone – ground – cross section -- India ink – 40x objective Canaliculi are tiny canals passing through the bone matrix, connecting one osteocyte to another, ultimately connecting them all to the Haversian canal. Compact bone Haversian canal osteocytic lacuna lamellae canaliculi end