Detection Circuit ENGR 1182.03 Pre Lab.

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Presentation transcript:

Detection Circuit ENGR 1182.03 Pre Lab

TrimPot Trim Pot Pin 3 to +5V Pin 3 Pin 1 to Ground Is a potentiometer (variable resistor) By varying the resistor, it creates a variable voltage source Total resistance (Pin 1 to Pin 3) = 10 k ohms Resistance at Pin 2 varies by turning the small adjustment screw ( 0 to +5V on Pin 2) Is used to calibrate the Binary Voltmeter circuit Trim Pot Pin 3 to +5V Pin 3 Pin 1 to Ground Pin 1 Pin 2 – Variable resistance controlled by screw Pin 2

Schematic

Fluorescein Detection Circuit The detection circuit performs four functions: Fluorescein excitation (with a blue LED) Fluorescein emission detection (green light photodetector) Converts fluorescence signal from analog to digital Displays a binary coded value 0-255 that is proportional to the amount of fluorescein in the detection well

Fluorescein Detection detection well excitation emission Acrylic chip Side view where Acrylic chip is sitting on top of the DAD blue LED photodetector

Optical Components Light Emitting Diode (LED) Photodetector Emits light proportional to current through diode Two uses of LED: Blue LED – Fluorescein excitation Red and Green LEDS are used to display the “Binary Number” at the output of the Binary Voltmeter LEDs are polarity dependent, the short wire has negative terminal whereas the longer wire has positive. Negative terminal Photodetector Resistance that varies with incident light Sensitive to specific wavelength (Green) Fluorescein emission detection EXTREMELY SENSITIVE TO POLARITY!

Fluorescein Detection Circuit Photo- Transistor 3 wires DAD Diode 2 wires Integrated Circuit ADC0804

Analog vs. Digital

Learning Objectives of Lab Students will learn about – Electronic circuit components Building the binary voltmeter circuit from a schematic drawing Troubleshooting electronic circuits Binary numbers and binary-decimal conversion