Safety in the Lab

Emergency Evacuation Instructions Please make sure that all Bunsen burners are off and gas valves are turned closed. Once a person has walked out of the room turn left down the hallway Then turn left to go down the stairs Once on the second floor, approach the exit to the stadium Go down the stairs to the football field

SCIENCE LAB THEATRE PRESENTS: DO’S AND DON’TS IN THE LABORATORY

Guess which students are following lab rules. Which rules are they breaking?

WRONG: Horseplay in the lab

WRONG: Pouring water into acid

WRONG: No Goggles No Apron

WRONG: Eating and drinking in the lab

WRONG: Carrying microscope with one hand Sandals in the lab

CORRECT: Holding microscope with both hands

WRONG: Handling broken glassware

WRONG: No goggles, Heating test tube toward you

CORRECT: Goggles, apron on, Heating test tube away from you

WRONG: Picking up beaker without using tongs; not wearing goggles

WRONG: Not assuming hot plate is hot!

CORRECT: Apron, goggles on Picking up beaker with tongs

WRONG: Long hair not tied back

WRONG: Smelling chemicals

RIGHT: Wafting chemicals

WRONG: WRONG: Horseplay in the lab

CORRECT: Apron, goggles on Holding beaker with tongs.

Locating safety equipment Go through the room with your partner and find each of these things listed. When you have found them in the classroom draw them accordingly on your classroom map  Lab shower  Eye wash  Fire Extinguisher  Fire blanket  Map of Emergency escape route

Types of Hazardous Chemicals Corrosive Flammable Toxic Reactive

Corrosive Corrosive to metals and injures body tissues Ex: Acids – Sulfuric Acetic, Phosphoric, Hydrochloric Bases – Sodium Hydroxide, Potassium hydroxide, Ammonia

Flammable Will burn Readily Ex: Acetic Acid, Solvents Acetone, Benzene, Ethanol Gases Propane, Acetylene

Toxic Poisonous Routes of entry Inhalation Ingestion Absorption Injection Chronic Effects Benzene = Leukemia

Toxic “Dose makes the poison” 1 aspirin = no problem, 100 aspirin = die Determined by Scientific research Increase dose until 50% of rats die = LD50 12 g of Sugar for 1lb rat = kill 50% of rats HCN –Hydrogen Cyanide LD50 = 4mg/kg LD 50s from MSDS

Carcinogen Causes cancer! Ex: Benzene Maybe Causes cancer: Formaldehyde Plastics – BPA Ethidium Bromide or EtBr (used in DNA Gel Electrophoresis)

Reactive Explodes, violently polymerizes, form explosive peroxides or react violently w/water or atmosphere Explosive Ammonium nitrate, Benzoyl Peroxide, Sodium Azide Oxidizer Chlorine Gas, 30% Hydrogen Peroxide, Zinc Nitrate, Potassiumchlorate/nitrate/permanganate Dry Ice + coke = explosion

Hazardous Material in the Home The U.S. Environmental Protection Agency estimates average household generates 20 pounds of hazardous waste per year Store hazardous materials in their original containers. Keep a bag of cat litter, sand, or sawdust near the storage area to soak up any spilled chemical from a broken or leaking container.

Hazardous Material in the Home Always store Flammable materials in the Garage never in the house. Keep Alcohols away from stoves and Grills Make sure to dispose of hazardous wastes properly to the City of El Paso Environmental Services Never Mix Bleach with Acid Toilet Bowl Cleaners, Vinegar, or Ammonia

Labels Always make sure that all chemicals, beakers, test tubes, and containers have labels.