Feb 18 & 19, 2009 ©2002-09 Thayumanasamy Somasundaram 1 X-Ray Facility Overview IMB X-Ray Facility (IMB XRF) 410-413 Kasha Laboratory www.sb.fsu.edu/~xray.

Slides:

Advertisements

Similar presentations

S.J. Coles a, P.N. Horton a, M.B. Hursthouse a, L. Male a, R.A. Stephenson a, W. Clegg b, R.W. Harrington b, L. Russo b. a School of Chemistry, University.

Advertisements

Customer Service through Technology Michael Jordan, Director of Operations ESP Computer Services …or How Programming and Technology Can Contribute to Better.

Overview of the Chemical Threat Laboratory of Texas William Edgemond, Ph.D. Texas Department of State Health Services October 2013.

Lecture 11. Microscopy. Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single or.

UWO Nanofabrication Facility and Science Studio. Facility to be hooked into Science Studio: Western Nanofabrication Facility, University of Western Ontario.

Synchrotron Diffraction. Synchrotron Applications What? Diffraction data are collected on diffractometer beam lines at the world’s synchrotron sources.

X-ray sources Sealed tubes - Coolidge type common - Cu, Mo, Fe, Cr, W, Ag intensity limited by cooling req'ments (2-2.5kW) Sealed tubes - Coolidge type.

Electron Microscopy Chelsea Aitken Peter Aspinall

Wayne Lewis Australian Synchrotron Beamline Controls Design and Implementation.

Structural Genomics – an example of transdisciplinary research at Stanford Goal of structural and functional genomics is to determine and analyze all possible.

Pathways Bioinformatics & Biomolecular Center at the City College of New York Marshak Science Building, Room 1102 Tel: 212/ Fax: 212/

Alchemist TM II: Capacity, reproducibility and efficiency of an high-throughput crystallization screen preparation system. Craig Sterling Director of Business.

(X-Ray Crystallography) X-RAY DIFFRACTION. I. X-Ray Diffraction  Uses X-Rays to identify the arrangement of atoms, molecules, or ions within a crystalline.

Hanging Drop Sitting Drop Microdialysis Crystallization Screening.

1 Licensing and Start-Ups at RIT Varda N. Main Director, Technology Licensing Office HTR Tech Entrepreneur Week October 21, 2004.

A U.S. Department of Energy Office of Science Laboratory Operated by The University of Chicago Argonne National Laboratory Office of Science U.S. Department.

Digital Radiography Chapter 22. History of Digital Radiography Slower process of conversion because no pressing need to convert to digital radiography.

Instrumentation - making & detecting x-rays Read Roe - Chap 2 through (ignore neutrons)

How to Go Digital (Advanced) Press Space Bar or Click Mouse to Advance.

Tools Used in Science. Beaker Used to hold liquid & pour Do not drop or strike *Do not sit on edge of table.

Working With Images, Sound, and Video Mark Grabe.

X-rays are electromagnetic waves (like light) and have a wavelength about the size of atoms. From

23 May June May 2002 From genes to drugs via crystallography 19 May 1996 Experimental and computational approaches to structure based.

Cryo Porter …for Secure and Ease of Transportation and Storage of your specimens Product Presentation.

Join the conference call by dialing the conference number in your Invitation or Reminder s. Please put your phone on mute. Please stand by! The webinar.

Corporate Overview. Established in 1999 as development partner of IBM California based with R&D facility located in Timisoara, Romania Grown to over 270.

IMB X-Ray Facility XRF User Fees For Aug 2009 – Jul 2010 Thayumanasamy Somasundaram Version 1.2; August 18, 2009 Contact Information:

V e RSI Victorian eResearch Strategic Initiative VBL Introduction Crystal 25 Rev 1.2.

animations/x-ray_diffraction.html.

Tools Scientists Use. Computer Description: Electronically find information and solve problems Function: Creates graphs, solves complex equations, analyze.

Digital Media Exam Review SM1001 Digital Media, Semester A, 2011 School of Creative Media © Week 13, 2011.

Lesson 16 CCD Detectors Visit the Lab. Charged Coupled Device The ccd is essentially a digital camera To keep noise down it is cooled It is almost realtime.

September 19, 2002CSG - Seattle1 Toward Sustainable Models for Funding IT in Higher Education Common Solutions Group September 19, 2002 Jack McCredie.

1 The Laboratory for MultiScale Imaging (LMSI) A Shared Resource for Interdisciplinary Research and Training Instrumentation  Scanning Electron Microscopy.

Postgraduate Computing Lectures Applications I: Overview 1 Applications: Overview Symbiosis: Theory v. Experiment Theory –Build models to explain existing.

Macromolecular Crystallography Summary of the July 18, 2007 Breakout Session.

Motor driven incident slits (define the incident x-ray beam size) Synchrotron x-ray upstream beam aperture CCD camera Video camera Long working distance.

Computer Parts And Components OUTPUT DEVICES. Output Devices A display device is an output device that visually conveys texts, graphics and video information.

X RAY CRYSTALLOGRAPHY. WHY X-RAY? IN ORDER TO BE OBSERVED THE DIMENTIONS OF AN OBJECT MUST BE HALF OF THE LIGHT WAVELENGHT USED TO OBSERVE IT.

Macromolecular Synchrotrons Protocols and Techniques Thayumana “Soma”sundaram Institute of Molecular Biophysics Florida State University.

“hands-off” Screening with TOPAZ Chips For the purposes of testing both crystallization behavior and capability of the “tray goniometer”, chips were built.

Sorting Technologies for CCA Treated Wood. Objective To design and implement an automated system to effectively sort CCA treated wood from other wood.

An Introduction to Mosflm (1) what Mosflm does (2) where it fits in the crystallography process (3) run through a typical job (4) introduction to the CCP4.

Phase Contrast sensitive Imaging

Understanding mass transport in fuel cells using neutron imaging

Diffraction Image Library F. Remacle & G. Winter.

Using Technology to Study Cellular and Molecular Biology.

Goals Structural Biology Collaboratory Allow a team of researchers distributed anywhere in the world to perform a complete crystallographic experiment.

BUILDING BUILDING A USER-FRIENDLY BEAMLINE Aina Cohen and Paul Ellis.

Training Workshop in use of Synchrotron Radiation and CCP13 Software for Non-Crystalline Diffraction / Fibre Diffraction 23 rd -24 th November 1999 at.

Purpose: Provide support to create, enhance and maintain laboratory support systems required to investigate, identify and mitigate actions in response.

Effectively Communicating in the World of Shared Resources T. “Soma” Somasundaram X-Ray Core Facility Florida State University Tallahassee, FL 32306

Improving Canadian Capacity for Predicting the Impacts of Natural and Human Disturbances on Boreal Water Resources Canadian Aquatic Laboratory for Interdiscplinary.

Bringing structural biology services through collaboration.

Digital Media Exam Review SM1001 Digital Media, Semester A, 2009 School of Creative Media © Week 13, 2010.

Soft Materials Research Center (SMRC) facilities Major Capabilities – Structural analysis X-ray diffraction facility Freeze-fracture transmission electron.

Kent State University, Kent, Ohio Liquid Crystal Institute

Biomolecular X-ray Crystallography Facility

X-Ray Facility Overview [BCH 4053]

Autoprocessing updates at the MX beamlines

X-Ray Facility Overview

Good Lab Etiquettes & Procedures

Cryo-EM Services Cryo-EM Services in Creative Biostructure.

Good Lab Etiquettes & Procedures

Welcome! X-Ray Facility, Kasha Laboratory

MM002plus/007HF comparison #33120: middle resolution range case:

eHTPX crystallization, shipping and future

Tools Used in Science.

Soma’s Synchrotron Notes

Presentation transcript:

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 1 X-Ray Facility Overview IMB X-Ray Facility (IMB XRF) Kasha Laboratory

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 2 IMB X-Ray Facility (XRF)  IMB: Inter-disciplinary Institute  Structural Biology & Computational Biophysics  XRF: Core Facility [Est. 1993]  Open and Free Access  Safety & On-site Training Required

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 3 What XRF Can Do For You? CrystalDiffraction e - Density Structure Images courtesy of Michael Chapman Lab XRF can help you in Steps

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 4 X-Ray Generator & Mirror  Rigaku RU-H2R  Cu Rotating Anode  Brilliant Beam  Osmic Confocal Mirror  Wavelength Purity  High Flux

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 5 X-Ray Detectors Imaging Plate & CCD  Rigaku R-Axis IIc  Two 19cm 2 IPs  8 minute Read-out  MarCCD165  Single CCD  3 s Read-out

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 6 X-Ray (Cryo) Data Collection  MarCCD/CrystalClear  GUI  Automated  Analog»Digital  Oxford CryoSystem  Collect at 100°K  Protects Crystals  Sharper eDensity

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 7 CryoCrystallography  Flash Cool in Stream For Immediate Collection Ease of Handling  Flash Cool in Liquid For Storage For Shipping & Transport Flash Cool in Stream Flash Cool in Liquid

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 8 Amenities & Accessories  Crystal Room  Cold Room  Variable T Incubator  Digital Imaging  Synchrotron Time  Microscopes  Cryo Tools  Computer Licenses  3D Graphics  Sample Tools

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 9 Crystal & Data Services  Screening & Crystallization  Data Collection Strategy  Crystal Storage & Transport  Synchrotron Time & Usage  Data Processing & Visualization  Data Interpretation  Data Storage & Extraction

Feb 18 & 19, 2009 © Thayumanasamy Somasundaram 10 XRF-Contact Thayumanasamy “Soma”sundaram 414 Institute of Molecular Biophysics Florida State University Tallahassee, FL Phone: | Fax: Web: