Alex Makowski Michael Hwang Jenny Lu Dr. John Wikswo

Slides:

Advertisements

Similar presentations

Zohora Iqbal PhD student. My Education Path Undergraduate – Las Positas/Chabot College (2.5 years) – UC Berkeley (2.5 years) Working at start-up – Ion.

Advertisements

Microfluidic Glucose Sensor Senior Design Group 4 Kristen Jevsevar Jason McGill Sean Mercado Rebecca Tarrant Advisor: Dr. John Wikswo, Dr. David Cliffel.

Electrolysis project Electrode: Is an electrical conductor that is used to pass current through an electrolyte.

Biocompatibility Many implants employ biomaterials that aren’t found in high concentrations in the body and are in turn incompatible with the site These.

The Effect of Electrical Stimulation on in vitro Diabetic Ulcer Models Team ELECTRODE: Evaluating Linear-Radial Electrode Conformations for Tissue Repair.

Cell isolation: Procedure and Troubleshooting

Analysis of Simple Cases in Heat Transfer P M V Subbarao Professor Mechanical Engineering Department I I T Delhi Gaining Experience !!!

Dr. Mansour Al Hoshan TiO 2 Nanotubes Arrays fabricated by anodizing process.

Figure S1. ID#1 Lipid Metabolism, Small Molecule Biochemistry, Drug Metabolism.

Solar Hydrogen Project Group Update 29 th July 2009.

ES 202 Fluid and Thermal Systems Applications of Thermodynamics and Fluid Mechanics.

Three-dimensional microfabricated bioreactor and closed-loop control system Alex Makowski Michael Hwang Jenny Lu Dr. John Wikswo.

A Novel Device for Controlling D. discoideum Movement with an Electric Field The Janetopoulos Lab, Department of Biological Sciences Arunan Skandarajah.

A guide to modelling cardiac electrical activity in anatomically detailed ventricles By: faezeh heydari khabbaz.

Done by: Guan Ruofei 3P3 (6)

Read Sections 8.3, and 8.4 before viewing the slide show.

PREPARATION OF ZnO NANOWIRES BY ELECTROCHEMICAL DEPOSITION

Microfluidics Technology Fair, October 3, 2006 Parallel Integrated Bioreactor Arrays for Bioprocess Development Harry Lee, Paolo Boccazzi, Rajeev Ram,

Microfluidic System for Automatic Cell Culture Chun-Wei Huang, Song-Bin Huang, Gwo-Bin Lee Department of Engineering Science, National Cheng Kung University,

Evaluating cell matrix stiffness with a multiphoton confocal microscope-optical tweezer setup Berney Peng, Carlo Alonzo, Lawrence Xia, Lucia Speroni, Irene.

Development and Characterization of Device for Galvanotactic Response in D. discoideum The Janetopoulos Lab, Department of Biological Sciences Devin Henson.

Alex Makowski Michael Hwang Jenny Lu Dr. John Wikswo

指導老師:許藝菊學生:邱建龍介電電泳（DEP）基於微流體粒子分離器Dielectrophoresis (DEP) Based Microfluidic Particle Separator.

E.L.E.C.T.R.O.D.E. Evaluating Linear-Radial Electrode Conformations for Tissue Repair and Optimizing a Device for Experimentation Team Members: Sagah.

Electroplating. The electroplating of an object with metal is accomplished using an electrolytic cell. There are some basic rules for electroplating.

Minibioreactors -> Volumes below 100 ml Characterized by: -> area of application -> mass transfer -> mixing characteristics.

THE NEED FOR NANOMATERIAL EVALUATION IN A PHYSIOLOGICALLY RELEVANT MODEL: CONNECTING ENVIRONMENTAL VARIABLES AND NM BEHAVIOR TO TOXICOLOGICAL RESPONSES.

Instrumented NanoPhysiometer for High Throughput Drug Screening D. Michael Ackermann, Jon Payne, Hilary Samples, James Wells.

Group 3: Microfluidic System for Galvanotaxis Measurements Team Members: Arunan Skandarajah and Devin Henson Advisors: Dr. Janetopoulos, Dr. John Wikswo,

Design of an integrated microfluidic system for culturing patterned tissue Allyson Fry 1, Bryan Gorman 1, Jonathan Lin 2, William Wong 1 Advisors: John.

Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS) Team Members: Allyson Fry Bryan Gorman Jonathan Lin William Wong Advisor: Dr. John P.

Design Team 8: Fluorescent Detection using Optical Fibers with Cardiac Myocytes Team Members: Paul Clark Martin Garcia Chris Gorga John Ling III Giordano.

 Electric Current- net movement of electric charges in a single direction ◦ Example- powering electronics.

Three-dimensional microfabricated bioreactor and closed-loop control system Alex Makowski Michael Hwang Jenny Lu Sam Cassady Dr. John Wikswo.

Group 3: Microfluidic System for Galvanotaxis Measurements Team Members: Arunan Skandarajah and Devin Henson Advisors: Dr. Janetopoulos, Dr. John Wikswo,

P15080: Flow Culture System Multi-Disciplinary Senior Design Problem Definition Review September 11, 2014 Collin Burkhart – Lead Mechanical Engineer Katelyn.

BioMEMS: Microfluidics Optimization & Cell Culture Maintenance D. Michael Ackermann, John Payne, Hillary Samples, James Wells Group # 23 Advisor: Franz.

Fully-integrated microfluidic chips capable of performing DNA amplification from RNA virus, sample transportation, capillary electrophoresis separation,

Acellular microfluidic lung on a chip design for gas exchange Introduction Design Methods Conclusions Acknowledgements Mathematical Analysis Integration.

FLUORESCENT DETECTION USING OPTICAL FIBERS WITH CARDIAC MYOCYTES DESIGN GROUP EIGHT FLUORESCENT DETECTION USING OPTICAL FIBERS WITH CARDIAC MYOCYTES Paul.

Kristen Jevsevar Jason McGill Sean Mercado Rebecca Tarrant.

Instrumented NanoPhysiometer for High Throughput Drug Screening D. Michael Ackermann, Jon Payne, Hilary Samples, James Wells.

Microfluidic Glucose Sensor Senior Design Group 4

Three-Dimensional Closed-Loop Microfabricated Bioreactor Michael Hwang, Jenny Lu, Alex Makowski, Advisors: Lisa McCawley 1, Dmitry Markov 2, Phil Samson.

Maryakhina V.S., Gun’kov V.V. Orenburg state university, Russia Saratov fall meeting

Development of Microfluidic Glucose Sensors BME 273: Kristen Jevsevar, Jason McGill, Sean Mercado, Rebecca Tarrant Advisors: Jennifer Merritt, Dr. John.

Microfluidic Glucose Sensor Senior Design Group 4 Kristen Jevsevar Jason McGill Sean Mercado Rebecca Tarrant.

Microarray: An Introduction

AGROFARM WEATHER MONITOR USING LabVIEW. INTRODUCTION Innovation in agriculture field Implementing instrumentation tech. in agricultural field The quantity.

History of Computers and Performance David Monismith Jan. 14, 2015 Based on notes from Dr. Bill Siever and from the Patterson and Hennessy Text.

Chapter A2 A2.4 – Voltaic Cells. Voltaic cells  The focus of our studies on metals has so far been focused on what’s happening on the surface of the.

Companion PowerPoint slide set Obesity-associated breast cancer risk: a role for epigenetics? This teacher slide set was created by Dana Haine, MS, of.

Introduction to Animal Tissue culture

Name- Sonu kumar Class- 8th A Roll no - 833

Fig. 3. ATME inhibits VEGF-induced invasion and tube formation of endothelial cells. (A) Effect of ATME on HUVEC invasion by using the Transwell culture.

FP420 Detector Cooling Thermal Considerations

7-2: Electric Current.

A Tribute to Alan Hall: Remembrances from Hall Lab Members

3-Valve Pumping Sequence 4-Valve Pumping Sequence

Introducing Current Electricity

Volume 114, Issue 2, Pages (February 1998)

Steady-State Heat Transfer (Initial notes are designed by Dr

Advantages access the applications in any place, any time. They do not need a wired connection. use both telephone signal and WiFi (for high end models)

Charlotte H. E. Weimar, Emiel D

Integrating Science, Engineering, and Technology

Charlotte H. E. Weimar, Emiel D

Volume 103, Issue 11, Pages (December 2012)

Fig. 1. Iontophoretic devices used for the delivery of cytotoxic agents to solid tumors. Iontophoretic devices used for the delivery of cytotoxic agents.

Volume 103, Issue 11, Pages (December 2012)

Presentation transcript:

Alex Makowski Michael Hwang Jenny Lu Dr. John Wikswo Three-dimensional microfabricated bioreactor and closed-loop control system Alex Makowski Michael Hwang Jenny Lu Dr. John Wikswo

Ultimate Project Goals Computer modulated growth of tissue structures within microfluidic devices. Possible uses include drug testing and dose determination, bypassing several stages of FDA trials.

Problem Statement- Design progress is blocked on three distinct fronts: Sensors are required to provide computer with necessary information. Bioreactor design limits the quantity and quality of cell morphology within the device. Previously used cells (primary human fibroblasts) do not easily form tissue-like structures.

Subsequent Requirements: Choose or design an appropriate sensor for pH measurements (most needed to determine cell metabolism and health). Choose a cell line that will exhibit some kind of morphological change under successful conditions. Redesign the bioreactor to incorporate new cell line and maximize efficiency of pH sensors.

Previous cell lines used… Primary human fibroblast and endothelial cells High cost Lower passages Difficult to exhibit 3D morphology Need morphology as control

Why use MCF10A cell line? Human breast epithelial cell line (MCF10A) Have in stock Less expensive Forms acinar morphology Illustration courtesy of Weaver Lab

Cell Culture Model of Epithelial Acini Confocal Imaging of Acini from 3-D matrigel cultures Illustration courtesy of Cassio Lynm, JAMA

Simplified Experimental Setup

Electrical connection for tubular Ti electrode Copper wire Copper plate Titanium tube Slide Courtesy of Sayed A. M. Marzouk United Arab Emirates University

Electroplating the inner wall of Ti tube with IrO2 Tygon tube To potentiostat Titanium tube to be coated (Working electrode) Pt tube (counter electrode) Pt tube (counter electrode) pump Electroplating solution Slide Courtesy of Sayed A. M. Marzouk United Arab Emirates University

Improvements over pH Optical fiber-type sensor Improved response time (6 s vs. 2-3 min.) Important for frequent measurements Allows faster recalibration No stagnant volume T-junction for fiber creates a volume around sensor with little or no convective flow Lack of convection  diffusion-limited analyte transport  even longer response time